FlyBase Allele Report: Dmel\r2d2[1]

General Information

Symbol

Dmel\r2d2¹

Species

D. melanogaster

Name

FlyBase ID

FBal0151615

Feature type

allele

Associated gene

Dmel\r2d2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Kalidas et al., 2008)

Mutagen

P-element activity

(Kalidas et al., 2008, Liu et al., 2003, Smith, 2003.12.15)

Progenitor genotype

P{EP}Herp^EP2450

(Kalidas et al., 2008, Smith, 2003.12.15)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 5kb deletion that removes the entire open reading frame.

(Kalidas et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | conditional

(Mueller et al., 2010)

abnormal immune response | recessive

(Kemp et al., 2013)

lethal | embryonic stage | maternal effect | recessive

(Kalidas et al., 2008)

lethal | embryonic stage | recessive

(Kalidas et al., 2008)

suppressor of variegation | dominant

(Fagegaltier et al., 2009)

Phenotype Manifest In

egg chamber

(Kalidas et al., 2008)

follicle cell

(Kalidas et al., 2008)

stalk follicle cell

(Kalidas et al., 2008)

stalk follicle cell | somatic clone

(Kalidas et al., 2008)

Detailed Description

Statement

Reference

Compared with wild-type, r2d2¹ mutant flies show increased sensitivity to Invertebrate Iridescent Virus type 6 (IIV-6) infection.

(Kemp et al., 2013)

r2d2¹ mutants display a reduction in dsRNA processing and a subsequent ~40% reduction in siRNA levels.

(Marques et al., 2010)

r2d2¹ mutant flies show severely compromised survival when infected with vesicular stomatitis virus, and die within 12 days of infection. Viral titres are significantly higher compared to controls.

(Mueller et al., 2010)

r2d2¹/+ significantly suppresses the position effect variegation of the Sb¹ allele that is seen in the T(2;3)Sb^V chromosome.

r2d2¹/r2d2¹ suppresses the position effect variegation of the w gene that is seen in the In(1)w^m4 chromosome.

(Fagegaltier et al., 2009)

Only 14 +/- 1.5% of homozygous animals hatch.

Only 76% of eggs are fertilised in the r2d2¹ strain (as assayed by incorporation of the sperm tail). Of the fertilised embryos, only 19% survive through the gastrulation stage, with many showing no signs of nuclear divisions.

The average number of progeny from mutant males mated to wild-type females is not different from that of wild-type controls. The average number of progeny from wild-type males mated to mutant females is reduced compared to wild type. The average number of progeny from mutant males mated to mutant females is reduced compared to both wild-type controls and compared to the number of progeny from wild-type males mated to mutant females.

Homozygous females have a number of defects in the ovaries; 35% of cysts lack the stalks that normally separate adjacent follicles, while in 15% of cases, compound follicles are seen where two follicles are fused together. Large gaps in the follicle epithelium are also seen (disruptions in the follicle cell epithelium are seen in more than 50% of stage 9 follicles), which may be large enough for germ cells to extrude out of the egg chamber. In some places there is a double layer of follicle cells in the same region of the epithelium. 29.3% of egg chambers degenerate.

Homozygous clones in the follicle cells result in defects in the formation of follicle stalks. Homozygous clones in the female germline do not cause defects in the follicle stalks.

(Kalidas et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

r2d2[+]/r2d2¹ is a non-enhancer of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

NOT Suppressor of

Statement

Reference

r2d2[+]/r2d2¹ is a non-suppressor of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

Other

Statement

Reference

Dcr-1^Q1147X/Dcr-1[+], r2d2¹ has female sterile phenotype

(Kalidas et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

r2d2¹ has egg chamber phenotype, enhanceable by Dcr-1^Q1147X/Dcr-1[+]

(Kalidas et al., 2008)

Suppressor of

Statement

Reference

loqs^f00791/r2d2¹ is a suppressor of eye phenotype of w^RNAi.GMR

(Marques et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The eyes of w^dsRNA.GMR transgenic female flies homozygous for r2d2¹ are similar to that of wild-type.

(Fukunaga et al., 2012)

The survival of roX1^ex33A Df(1)roX2Δ adult males is not significantly altered if they are also heterozygous for r2d2¹.

(Menon and Meller, 2012)

r2d2¹ mutant flies homozygous for AGO2^{T:Zzzz\FLAG,T:Ivir\HA1} are fertile.

(Okamura et al., 2011)

r2d2¹ mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^f00791 r2d2¹ double mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^f00791 r2d2¹ double mutants display a reduction in dsRNA processing and a subsequent ~80% reduction in siRNA levels.

(Marques et al., 2010)

The female fertility defect seen in r2d2¹ females is dramatically enhanced by Dcr-1^Q1147X/+; the females are completely sterile and the ovaries are severely disorganised with completely fused follicles.

(Kalidas et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

r2d2¹ is rescued by r2d2^+t5.6

(Kalidas et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

54575

y¹ w^*; r2d2¹ P{neoFRT}40A/CyO, amos^Roi-1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

R2D2¹

(Kemp et al., 2013, Han et al., 2011, Marques et al., 2010, Mueller et al., 2010)

r2d2¹

(Eyk et al., 2019, Chak et al., 2015, Liang et al., 2015, Fukunaga et al., 2012, Menon and Meller, 2012, Dufourt et al., 2011, Okamura et al., 2011, Fagegaltier et al., 2009, Deddouche et al., 2008, Kalidas et al., 2008, Förstemann et al., 2007, Horwich et al., 2007, Josse et al., 2007)

Name Synonyms

r2d2 mutant

(Liu et al., 2003)

Secondary FlyBase IDs

References (21)

Report Sections

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