FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\p53E8
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General Information
Symbol
Dmel\p53E8
Species
D. melanogaster
Name
FlyBase ID
FBal0151647
Feature type
allele
Associated gene
Dmel\p53
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Lee et al., 2003)
Mutagen
Delta2-3 transposase
(Lee et al., 2003)
Progenitor genotype
P{EP}p53EP-1
(Lee et al., 2003)
Cytology
Description

Excision of the P{EP} element, resulting in a deletion that removes most of the promoter region of p53.

(Lee et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  testicular cancer
      CEA
      (Napoletano et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      p53E8 homozygotes exhibit a severe increase in the proportion of hyperplastic testes, as compared to heterozygous or wild-type controls; these testes exhibit an accumulation of Bam-positive maturing spermatogonia and a significant decrease in necrosis (as shown by the number of spermatogonial cysts bearing TUNEL-positive cells) at their apical tip, but no changes in the size of differentiating spermatogonia and spermatocytes, as compared to controls; mutant spermatids undergo meiosis, but some onion-stage nebenkern spermatids showed micronuclei or had no apparent nuclei, which suggests a defect in chromosome segregation; cystic bulges are rarer and the migrating individualization complexes are often morphologically abnormal, as compared to controls.

      (Napoletano et al., 2017)

      Wing and eye imaginal discs of p53E8 animals do not show an increase in the number of apoptotic cells following irradiation (in contrast to wild-type discs), but following irradiation, the number of cells undergoing mitosis is significantly decreased in both p53E8 and wild-type discs.

      (Lee et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Statement
      Reference

      cathD1, p53[+]/p53E8 has testis | adult stage phenotype

      (Napoletano et al., 2017)

      EndoGMB07150, p53[+]/p53E8 has testis | adult stage phenotype

      (Napoletano et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      cathD1/cathD1, p53E8/+ adults or EndoGMB07150/Df(2R)BSC699, p53E8/+ adults, but not car1/car1, p53E8/+ adults or DNaseIIlo/+, p53E8/+ adults, exhibit a significant proportion of hyperplastic testes, as compared to cathD1/cathD1 adults, p53E8/+ adults, or wild-type controls.

      (Napoletano et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      p53E8 is partially rescued by Scer\GAL4nanos.PU/p53UAS.Dp53

      (Napoletano et al., 2017)

      p53E8 is partially rescued by p53UAS.DΔNp53/Scer\GAL4nanos.PU

      (Napoletano et al., 2017)
      Comments

      Both the significant proportion of hyperplastic adult testes in p53E8 homozygotes and the increased necrosis at the apical tip of those testes are rescued by the expression of either p53Scer\UAS.Dp53 or p53Scer\UAS.DΔNp53 under the control of Scer\GAL4nos.PU.

      (Napoletano et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dmp53E8
      (Lee et al., 2003)
      p53E8
      (Zhou et al., 2021, Napoletano et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)