Open Close
General Information
Symbol
Dmel\p53E8
Species
D. melanogaster
Name
FlyBase ID
FBal0151647
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Excision of the P{EP} element, resulting in a deletion that removes most of the promoter region of p53.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

p53E8 homozygotes exhibit a severe increase in the proportion of hyperplastic testes, as compared to heterozygous or wild-type controls; these testes exhibit an accumulation of Bam-positive maturing spermatogonia and a significant decrease in necrosis (as shown by the number of spermatogonial cysts bearing TUNEL-positive cells) at their apical tip, but no changes in the size of differentiating spermatogonia and spermatocytes, as compared to controls; mutant spermatids undergo meiosis, but some onion-stage nebenkern spermatids showed micronuclei or had no apparent nuclei, which suggests a defect in chromosome segregation; cystic bulges are rarer and the migrating individualization complexes are often morphologically abnormal, as compared to controls.

Wing and eye imaginal discs of p53E8 animals do not show an increase in the number of apoptotic cells following irradiation (in contrast to wild-type discs), but following irradiation, the number of cells undergoing mitosis is significantly decreased in both p53E8 and wild-type discs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

cathD1/cathD1, p53E8/+ adults or EndoGMB07150/Df(2R)BSC699, p53E8/+ adults, but not car1/car1, p53E8/+ adults or DNaseIIlo/+, p53E8/+ adults, exhibit a significant proportion of hyperplastic testes, as compared to cathD1/cathD1 adults, p53E8/+ adults, or wild-type controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Both the significant proportion of hyperplastic adult testes in p53E8 homozygotes and the increased necrosis at the apical tip of those testes are rescued by the expression of either p53Scer\UAS.Dp53 or p53Scer\UAS.DΔNp53 under the control of Scer\GAL4nos.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)