FlyBase Allele Report: Dmel\p53[E4]

General Information

Symbol

Dmel\p53^E4

Species

D. melanogaster

Name

FlyBase ID

FBal0151648

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lee et al., 2003)

Mutagen

Delta2-3 transposase

(Lee et al., 2003)

Progenitor genotype

P{EP}p53^EP-1

(Lee et al., 2003)

Cytology

Description

Excision of the P{EP} element, resulting in a deletion that removes the first four exon of p53.

(Lee et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | oogenesis

(Park et al., 2019)

radiation resistant | oogenesis

(Park et al., 2019)

(Lee et al., 2003)

(Lee et al., 2003)

(Lee et al., 2003)

(Lee et al., 2003)

Phenotype Manifest In

germarium | oogenesis

(Park et al., 2019)

Detailed Description

Statement

Reference

Under normal conditions p53^E4 adults exhibit an increased cell death in germarium compared to controls. 6 hours after 3 Gy irradiation they do not exhibit an increased cell death rate in germarium, different than controls.

(Park et al., 2019)

Homozygous flies survive as well as controls up to 50 days, but after this the survival of p53^E4 flies is reduced compared to controls. The fertility of p53^E4 flies is half that of controls. p53^E4 flies are more sensitive to ionising radiation than control flies (as assayed by survival of animals exposed to radiation). Irradiated p53^E4 flies show a greater loss of macrochaetae than control flies (indicating greater genomic instability). Wing and eye imaginal discs of p53^E4 animals do not show an increase in the number of apoptotic cells following irradiation (in contrast to wild-type discs), but following irradiation, the number of cells undergoing mitosis is significantly decreased in both p53^E4 and wild-type discs.

(Lee et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

p53^E4 is a suppressor of increased cell death | conditional phenotype of Hr3^RA.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

p53^E4 is a suppressor of increased cell death | conditional phenotype of Hr3^RB.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

p53^E4 prevents the IR irradiation-induced increase in apoptosis seen in eye discs expressing Hr46^RA.Scer\UAS or Hr46^RB.Scer\UAS under the control of Scer\GAL4^GMR.PU.

(Kim et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dmp53 E4

(Kim et al., 2011)

Dmp53^E4

(Lee et al., 2003)

p53^E4

(Park et al., 2019, Hwang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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