FlyBase Allele Report: Dmel\Trxr1[+t10]

General Information

Symbol

Dmel\Trxr1^+t10

Species

D. melanogaster

Name

FlyBase ID

FBal0152149

Feature type

allele

Associated gene

Dmel\Trxr1

Associated Insertion(s)

Carried in Construct

P{Trxr1^+t10}

Key Links

Transgenic product class

genomic_DNA

(Mockett et al., 1999)

wild_type

(Mockett et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Mockett et al., 1999)

wild_type

(Mockett et al., 1999)

Mutagen

in vitro construct

(Orr et al., 2003, Mockett et al., 1999)

Progenitor genotype

Carried in construct

P{Trxr1^+t10}

Cytology

Description

10kb SacII genomic fragment encoding Trxr-1, its 2.5kb intron and approximately 4kb upstream and 1.5kb downstream from the coding region.

(Mockett et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

Trxr1

Encoded product / tool

Trxr1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response

(Mockett et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies carrying Trxr-1^+t10 show no change in life span compared to controls under normoxic conditions.

Flies carrying Trxr-1^+t10 show an increase in mean life span compared to controls under continuous exposure to 100% oxygen. Flies carrying Trxr-1^+t10 show increased survival compared to control flies after exposure to 100% oxygen for 3.1 days, followed by maintenance under normoxic conditions.

Flies carrying Trxr-1^+t10 show the same mean, median and maximum life span as control flies during paraquat exposure.

Flies carrying Trxr-1^+t10 show an 18% decrease in mean life span and up to 25% decrease in maximum survival time under starvation conditions, relative to controls.

(Mockett et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Trxr1^+t10 is a suppressor of short lived phenotype of Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32

(Radyuk et al., 2010)

Trxr1^+t10 is a suppressor of abnormal stress response phenotype of Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32

(Radyuk et al., 2010)

Sod1^+t2.4, Cat^tOa, Trxr1^+t10 is a suppressor of chemical sensitive phenotype of Ppcs¹

(Bosveld et al., 2008)

Sod1^+t2.4, Cat^tOa, Trxr1^+t10 is a suppressor of chemical sensitive phenotype of fbl¹

(Bosveld et al., 2008)

NOT Suppressor of

Statement

Reference

Sod1^+t2.4, Cat^tOa, Trxr1^+t10 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Ppcs¹

(Bosveld et al., 2008)

Sod1^+t2.4, Cat^tOa, Trxr1^+t10 is a non-suppressor of radiation sensitive phenotype of Ppcs¹

(Bosveld et al., 2008)

Sod1^+t2.4, Cat^tOa, Trxr1^+t10 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Ppat-Dpck⁴³

(Bosveld et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Trxr-1^+t10 substantially prolongs survivorship of flies expressing Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background, increasing survivorship by almost 60%. Trxr-1^+t10 also restores sulfhydryl levels and the GSH:GSSG ratio in these flies.

(Radyuk et al., 2010)

Simultaneous co-expression of Sod^+t2.4, Cat^tOa and Trxr-1^+t10 suppresses the sensitivity of Ppcs¹/Ppcs¹ flies to 5% H[[2]]O[[2]] and 100mM dithiothreitol, and results in increased resistance to 20mM paraquat.

Simultaneous co-expression of Sod^+t2.4, Cat^tOa and Trxr-1^+t10 restores survival of Ppcs¹/Ppcs¹ and fbl¹/fbl¹ larvae on medium containing 100mM cysteine to wild-type levels.

Simultaneous co-expression of Sod^+t2.4, Cat^tOa and Trxr-1^+t10 does not suppress the impaired ability of Ppat-Dpck⁴³ or Ppcs¹ homozygous adults to climb in a negative geotaxis assay.

The reduced survival rate of adults after exposure of larvae to 20Gy irradiation that is seen in Ppcs¹ homozygotes is not rescued by simultaneous co-expression of Sod^+t2.4, Cat^tOa and Trxr-1^+t10.

(Bosveld et al., 2008)

Expression of both Trxr-1^+t10 and Sod^+t2.4 does not result in an increase in life span. Expression of both Trxr-1^+t10 and Cat^tOa does not result in an increase in life span.

(Orr et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trxr-1^+t10

Trxr1^+t10

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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