FlyBase Allele Report: Dmel\Prx3[RNAi.UAS]

General Information

Symbol

Dmel\Prx3^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0249414

Feature type

allele

Associated gene

Dmel\Prx3

Associated Insertion(s)

Carried in Construct

P{UAS-Prx3.RNAi}

Also Known As

RNAi-dPrx3, UAS-RNAi-dprx3

Key Links

Transgenic product class

RNAi_reagent

(Radyuk et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Radyuk et al., 2010)

Mutagen

in vitro construct

(Radyuk et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Prx3.RNAi}

Cytology

Description

UAS drives expression of two copies a 649 bp Prx3 domain placed tail-to-tail with an intervening sequence to generate an RNAi Prx3 hairpin construct.

(Radyuk et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Prx3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response, with Scer\GAL4^da.G32

(Radyuk et al., 2010)

increased cell death, with Scer\GAL4^da.G32

(Radyuk et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 produced a phenotype that was indistinguishable from controls in terms of developmental timing, fertility, physical activity levels, or life span. However, exposure of young (10-15 day old) or middle-aged (30-40 day old) flies to paraquat or hydrogen peroxide reveals small but significant decreases in resistance to oxidants.

There is a slight increase in apoptosis in Prx3^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 flies relative to controls.

(Radyuk et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Prx3^RNAi.UAS has increased cell death phenotype, enhanceable by Prx5^EY02106

(Radyuk et al., 2010)

Suppressed by

Statement

Reference

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has short lived phenotype, suppressible by Trxr1^+t10

(Radyuk et al., 2010)

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has abnormal stress response phenotype, suppressible by Trxr1^+t10

(Radyuk et al., 2010)

NOT suppressed by

Statement

Reference

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^arm.PS has short lived phenotype, non-suppressible by Cat^{UAS.Tag:Mito(Oat)}, Scer\GAL4^arm.PS

(Radyuk et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^da.G32/Prx3^RNAi.UAS is an enhancer of short lived phenotype of Prx5^EY02106

(Radyuk et al., 2010)

Scer\GAL4^da.G32/Prx3^RNAi.UAS is an enhancer of increased cell death phenotype of Prx5^EY02106

(Radyuk et al., 2010)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.G32/Prx3^RNAi.UAS is a non-enhancer of abnormal stress response phenotype of Prx5^EY02106

(Radyuk et al., 2010)

Other

Statement

Reference

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has abnormal stress response phenotype

(Radyuk et al., 2010)

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^arm.PS has short lived phenotype

(Radyuk et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Under normal conditions, expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background shortens mean lifespan from 55-70 days to 12.8 days. The 'double mutant' flies appear normal for the first 9-11 days after eclosion, but then undergo a very rapid increase in mortality rate. In response to either hydrogen peroxide or paraquat treatment, the 'double mutant' exhibits diminished survivability similar to the Prx5^EY02106 mutant.

Expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background results in an accelerated pro-oxidizing shift in the redox state shortly before an early death: the GSH:GSSG ratio and protein sulfhydryl levels (measures of thiol homeostasis) decrease significantly. Other markers of oxidative stress or mitochondrial function are not affected notably.

Expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^arm.PS in a Prx5^EY02106 background shortens median lifespan. This phenotype is not rescued by co-expression of Cat^{Scer\UAS.T:mito1}.

Trxr-1^+t10 substantially prolongs survivorship of flies expressing Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background, increasing survivorship by almost 60%. Trxr-1^+t10 also restores sulfhydryl levels and the GSH:GSSG ratio in these flies.

Prx3^{dsRNA.Scer\UAS}, Scer\GAL4^arm.PS, Prx5^EY02106 flies show a dramatic increase in apoptotic cells in thoracic muscles, digestive tract and oenocytes compared to single mutants and controls.

(Radyuk et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Prx3^RNAi.UAS

Prx3^{dsRNA.Scer\UAS}

Prx3^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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