FlyBase Allele Report: Dmel\Prx5[EY02106]

General Information

Symbol

Dmel\Prx5^EY02106

Species

D. melanogaster

Name

FlyBase ID

FBal0156897

Feature type

allele

Associated gene

Dmel\Prx5

Associated Insertion(s)

P{EPgy2}Prx5^EY02106

Carried in Construct

Also Known As

dprx5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Prx5^EY02106

Cytology

Description

Insertion in the first exon.

(Michalak et al., 2008)

The P{EPgy2}CG7217^EY02106 insertion maps within a dicistronic processed transcript that encodes two non-overlapping open reading frames (CG7215 and CG7217).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:18,166,393..18,166,393 [-]

(Odnokoz et al., 2021, Radyuk et al., 2010, Michalak et al., 2008, Gene Disruption Project members, 2001-, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Prx5^EY02106/Prx5^EY02106 flies do not show significant differences compared to controls under exposure to oxidative stress (10mM paraquat).

(Khoshnood et al., 2016)

Compared with wild-type, Prx5^EY02106 mutants show increased resistance to septic infection with Bacillus subtilis. Prx5^EY02106 mutant flies are cleared from septic Escherichia coli infection significantly faster than controls.

(Radyuk et al., 2010)

There is a slight increase in apoptosis in Prx5^EY02106 flies relative to controls, localized to muscle, digestive tract and oenocytes.

(Radyuk et al., 2010)

Homozygous flies produce progeny that are largely arrested during early embryogenesis, at stage 8. This phenotype can be rescued if either of the parents is heterozygous.

Mutant flies show a modest reduction in mean life span compared to controls, largely due to an accelerated death rate during 5-10 days after eclosion. Subsequent to this period the mortality rate stabilises and the surviving flies reach a maximum life span similar to that of controls.

Homozygous flies show dramatically reduced viability following treatment with either hydrogen peroxide or paraquat compared to control flies.

Homozygous 10 day old flies show apoptotic activity in the oenocytes, digestive system (mostly midgut) and cardia when reared under normal conditions, whereas control flies show almost no discernible apoptosis under these conditions. If the flies are reared in the presence of paraquat, 10 day old mutant flies show higher levels of apoptosis in the oenocytes, midgut and flight muscles compared to control flies.

(Radyuk et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Prx5^EY02106 has short lived phenotype, enhanceable by Scer\GAL4^da.G32/Prx3^RNAi.UAS

(Radyuk et al., 2010)

Prx5^EY02106 has increased cell death phenotype, enhanceable by Scer\GAL4^da.G32/Prx3^RNAi.UAS

(Radyuk et al., 2010)

NOT Enhanced by

Statement

Reference

Prx5^EY02106 has abnormal stress response phenotype, non-enhanceable by Scer\GAL4^da.G32/Prx3^RNAi.UAS

(Radyuk et al., 2010)

Suppressed by

Statement

Reference

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has short lived phenotype, suppressible by Trxr1^+t10

(Radyuk et al., 2010)

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has abnormal stress response phenotype, suppressible by Trxr1^+t10

(Radyuk et al., 2010)

NOT suppressed by

Statement

Reference

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^arm.PS has short lived phenotype, non-suppressible by Cat^{UAS.Tag:Mito(Oat)}, Scer\GAL4^arm.PS

(Radyuk et al., 2010)

Enhancer of

Statement

Reference

Prx5^EY02106 is an enhancer of increased cell death phenotype of Prx3^RNAi.UAS

(Radyuk et al., 2010)

Other

Statement

Reference

Prx5^EY02106, Urm1ⁿ¹²³ has abnormal oxidative stress response phenotype

(Khoshnood et al., 2016)

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^da.G32 has abnormal stress response phenotype

(Radyuk et al., 2010)

Prx3^RNAi.UAS, Prx5^EY02106, Scer\GAL4^arm.PS has short lived phenotype

(Radyuk et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Prx5^EY02106/Prx5^EY02106 does not significantly alter survival rates of Urm1ⁿ¹²³/Urm1ⁿ¹²³ flies under oxidative stress, with double mutants surviving significantly longer than wild type or Prx5^EY02106/Prx5^EY02106 flies.

(Khoshnood et al., 2016)

Under normal conditions, expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background shortens mean lifespan from 55-70 days to 12.8 days. The 'double mutant' flies appear normal for the first 9-11 days after eclosion, but then undergo a very rapid increase in mortality rate. In response to either hydrogen peroxide or paraquat treatment, the 'double mutant' exhibits diminished survivability similar to the Prx5^EY02106 mutant.

Expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background results in an accelerated pro-oxidizing shift in the redox state shortly before an early death: the GSH:GSSG ratio and protein sulfhydryl levels (measures of thiol homeostasis) decrease significantly. Other markers of oxidative stress or mitochondrial function are not affected notably.

Expression of Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^arm.PS in a Prx5^EY02106 background shortens median lifespan. This phenotype is not rescued by co-expression of Cat^{Scer\UAS.T:mito1}.

Trxr-1^+t10 substantially prolongs survivorship of flies expressing Prx3^{dsRNA.Scer\UAS} using Scer\GAL4^da.G32 in a Prx5^EY02106 background, increasing survivorship by almost 60%. Trxr-1^+t10 also restores sulfhydryl levels and the GSH:GSSG ratio in these flies.

Prx3^{dsRNA.Scer\UAS}, Scer\GAL4^arm.PS, Prx5^EY02106 flies show a dramatic increase in apoptotic cells in thoracic muscles, digestive tract and oenocytes compared to single mutants and controls.

(Radyuk et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

15852

y¹ w^67c23; P{EPgy2}Prx5^EY02106 CG7215^EY02106

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG7217^EY02106

CG7217^EY021106

(Michalak et al., 2008)

Prx5^EY02106

(Khoshnood et al., 2016)

dprx5

(Radyuk et al., 2010, Radyuk et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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