FlyBase Allele Report: Dmel\Slik[kd.UAS]

General Information

Symbol

Dmel\Slik^kd.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0152296

Feature type

allele

Associated gene

Dmel\Slik

Associated Insertion(s)

Carried in Construct

P{UAS-Slik.kd}

Also Known As

UAS-slik^kd

Key Links

Transgenic product class

missense_variant

(Hipfner and Cohen, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Hipfner and Cohen, 2003)

Mutagen

in vitro construct

(Hipfner and Cohen, 2003)

Progenitor genotype

Slik^UAS.cHa

(Hipfner and Cohen, 2003)

Carried in construct

P{UAS-Slik.kd}

Cytology

Description

D176N mutation within the DFG triplet of kinase subdomain VII, creating a kinase inactive version of Slik.

(Hipfner and Cohen, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Slik

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | cell non-autonomous | third instar larval stage, with Scer\GAL4^ptc.PU

(Panneton et al., 2015)

Phenotype Manifest In

peripodial epithelium | cell non-autonomous | third instar larval stage, with Scer\GAL4^ptc.PU

(Panneton et al., 2015)

Detailed Description

Statement

Reference

Expression of Slik^kd.Scer\UAS under the control of Scer\GAL4^ptc.PU (using UAS-Dicer2 to enhance RNAi efficiency) in cells of the wing disc proper induces non-autonomous overproliferation of the overlying peripodial membrane cells in third instar larvae.

(Panneton et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Slik^kd.UAS has increased cell number phenotype, enhanceable by Scer\GAL4^ptc-559.1/BacA\p35^UAS.cHa

(Hipfner and Cohen, 2003)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1, Slik^kd.UAS has increased cell number phenotype

(Hipfner and Cohen, 2003)

Phenotype Manifest In

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1, Slik^kd.UAS has peripodial epithelium phenotype

(Hipfner and Cohen, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Slik^{dsRNA.Scer\UAS.5'UTR} to deplete endogenous Slik does not interfere with the ability of Slik^kd.Scer\UAS expression driven by Scer\GAL4^ptc.PU to induce non-autonomous overproliferation of the overlying peripodial membrane cells in third instar larvae.

(Panneton et al., 2015)

Xenogenetic Interactions

Statement

Reference

Genetic interaction (class, effect): increased cell number, UI { BacA\p35^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 } Genetic interaction (anatomy, effect): peripodial epithelium, UI { BacA\p35^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 } When slik^kd.Scer\UAS and BacA\p35^Scer\UAS.cHa are co-expressed under the control of Scer\GAL4^ptc-559.1 an increase in cell proliferation is seen in the wing disc epithelium and also in the peripodial layer that overlies the disc.

(Hipfner and Cohen, 2003)

When slik^kd.Scer\UAS and BacA\p35^Scer\UAS.cHa are co-expressed under the control of Scer\GAL4^ptc-559.1 an enhancement of the slik^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing.

(Hipfner and Cohen, 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Plkk1^kd.Scer\UAS

Slik^kd.Scer\UAS

Slik^kd.UAS

slik^kd.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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