FlyBase Allele Report: Mmus\Foxo1[AA.UAS]

General Information

Symbol

Mmus\Foxo1^AA.UAS

Species

M. musculus

Name

FlyBase ID

FBal0152412

Feature type

allele

Associated gene

Mmus\Foxo1

Associated Insertion(s)

Carried in Construct

P{UAS-mFoxo1-AA}

Transgenic product class

missense_variant

(Kramer et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kramer et al., 2003)

Mutagen

in vitro construct

(Kramer et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-mFoxo1-AA}

Cytology

Description

UASt regulatory sequences drive a constitutively active, mutated form of Mmus\Foxo1 (carries the amino acid replacements T24A and S253A).

(Kramer et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Foxo1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | larval stage, with Scer\GAL4^Act5C.PI

(Kramer et al., 2003)

abnormal locomotor behavior | larval stage, with Scer\GAL4^Act5C.PI

(Kramer et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

ommatidium, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Detailed Description

Statement

Reference

Expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^Act5C.PI early in larval development results in complete developmental arrest of the larvae, which remain in the first instar for up to 7 days. The feeding behaviour of these larvae is different from controls; the larvae expressing Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^Act5C.PI show a 3-4 fold increase in wondering at 48 and 72 hours after egg laying compared to control larvae. Expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^hs.PB early in larval development using heat shock treatment for 10 minutes every 24 hours inhibits larval growth. Upon removal from heat shock treatment the larvae remain in a state of developmental arrest until death. Expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a mutant eye phenotype characterised by a lack of ommatidia and mechanosensory bristles. Expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a significant reduction in the area of the ommatidia compared to control flies.

(Kramer et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Suppressed by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

NOT suppressed by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Ras64B^Val14.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Suppressed by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, suppressible | partially by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

NOT suppressed by

Statement

Reference

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, non-suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, non-suppressible by Ras64B^Val14.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Ras64B^Val14.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Mmus\Foxo1^AA.Scer\UAS and Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF almost completely obliterates the eyes. Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} partially rescues the eye phenotype caused by expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF; a lack of ommatidia and mechanosensory bristles is still seen. Co-expression of Akt1^Scer\UAS.cSa does not rescue the eye phenotype caused by expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF. Co-expression of Akt1^Scer\UAS.cSa and Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF results in ommatidia that are approximately the same size as in control flies.

(Kramer et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Foxo1^AA.Scer\UAS

Mmus\Foxo1^AA.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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