elB^3.3.1 noc^d64 double mutant ey clones do not show any overt phenotype in the adult antenna or in the most doral or ventral side of the head capsule. When located close to the DV boundary, clones induced overgrowths of the head capsule. The overgrowths are associated with ectopic bristles and consist mainly of mutant cells. However, in some cases, a few wild-type cells are detected in the outgrowths. Clones located in the presumptive antenna appendage or differentiating eye tissue do not show any apparent phenotype in terms of growth effects. When located in the region anterior to the morphogentic furrow, where their expression levels are higher, they caused tissue outgrowth. Mutant cells have a growth advantage over wild-type tissue since clones generating the outgrowths are much larger than those located elsewhere in the eye-antenna disc. Cell size is not affected. All overgrown tissues show increased proliferation. These local elevations are restricted to mutant cells. There is no increase in mitotic activity. There is not apparent accumulation of dying cells compared to wild-type surroundings. When a growth advantage is introduced by means of kiling the twin clones resulting from the FRT-mediated recombination, the extend and frequency of the overgrowths are even greater. These overgrowths very often include ectopic antennae and eye structures. In some cases, multiple ectopic antennae or eyes are detected within the overgrowths. Endogenous antennae and eyes are not affected.

A reduction of N protein levels in elB^3.3.1 noc^d64 double mutant ey clones, through expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Act5C} reduces the frequency of overgrowths per adult fly from 51.5% to 2.2%, without affecting cell viability.

A reduction of mam protein levels in elB^3.3.1 noc^d64 double mutant ey clones, through expression of mam^N.Scer\UAS under the control of Scer\GAL4^{Scer\FRT.Act5C} reduces the frequency of overgrowths per adult fly from 51.5% to 4.8%, without affecting cell viability.

The frequency of overgrowths observed in elB^3.3.1 noc^d64 double mutant ey clones is not affected when the JAK-STAT pathway is blocked by expression of dominant-negative dome^{ΔCYT.Scer\UAS} (under the control of Scer\GAL4^{Scer\FRT.Act5C}).

When elB^3.3.1; noc^d64 double homozygous somatic clones are generated in early 2nd instar larvae, they are recovered at much lower frequencies located from distal regions of the late theird instar wing or leg discs than are wild-type twin spot clones. Those that are recovered are rounded-up and appear to extrude from the disc epithelium. In contrast, these clones are recovered at the same frequency as their wild-type twin-spots in the proximal regions of wing and leg discs. In adult wings, clones are sometimes recovered as vesicles of mutant tissue between the wing surfaces. Few larvae with many large elB^3.3.1; noc^d64 double mutant 'minute⁺' clones (induced during 2nd instar in a 'minute-/+' background) survive to adulthood. Those that do have legs lacking distal elements (coxa, trochanter, femur, tibia, and tarsal segments) and exhibit an almost complete loss adult wing tissue, with the remaining tissue being heterozygous. These clones cause no obvious adult phenotype when they fall in the notum. When induced in early 3rd instar, these clones lead to loss of wing hinge, non-autonomous reductions in wing size, failure of leg segmentation and loss of trochanter.