FlyBase Allele Report: Dmel\park[P23]

General Information

Symbol

Dmel\park^P23

Species

D. melanogaster

Name

FlyBase ID

FBal0158832

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

P{EP}park^P23

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}park^P23

Cytology

Description

P{EP} is inserted in the coding region of park. As a result, the predicted protein product is truncated at amino acid 127.

(Pesah et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:21,196,263..21,196,265 [-]

(Pesah et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size

(Ottone et al., 2011)

female sterile

(Ottone et al., 2011)

partially lethal - majority die

(Ottone et al., 2011)

some die during pupal stage

(Ottone et al., 2011)

Phenotype Manifest In

egg chamber

(Ottone et al., 2011)

oocyte

(Ottone et al., 2011)

ovary

(Ottone et al., 2011)

Detailed Description

Statement

Reference

park^P23/park^P23 mutant females display large, abnormal egg chambers which contain more than 16 germ-line cells. Large follicles contain multiple 16 germ-cell cysts, enveloped by a single follicle cell epithelium. In the large follicles, one oocyte is usually observed located at the posterior, while the other oocytes are medially or anteriorly located.

The number of mature oocytes oviposited from park^P23 mutant females mated to wild-type males is 1.2/mother, and the rate of larval hatching is 0.

park^P23 mutants eclose later than heterozygous controls. Adults display a body mass reduction of approximately 30% in both sexes.

(Ottone et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

park^P23 has partially lethal - majority die | pupal stage phenotype, suppressible by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Ottone et al., 2011)

park^P23 has abnormal body size phenotype, suppressible by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Ottone et al., 2011)

park^P23 has female sterile phenotype, suppressible | partially by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Ottone et al., 2011)

eIF4E1⁰⁷²³⁸/eIF-4E[+], park^P23 has viable | pupal stage phenotype, suppressible by Thor^Δ1034-2617

(Ottone et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

park^P23 has egg chamber phenotype, suppressible by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Ottone et al., 2011)

park^P23 has ovary phenotype, suppressible by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Ottone et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The pupal lethality observed in park^P23/park^P23 mutant animals is suppressed in a eIF-4E⁰⁷²³⁸/+ background. Pupal lethality is restored in Thor^Δ1034-2617/Thor^Δ1034-2617; park^P23, eIF-4E⁰⁷²³⁸/park^P23 animals.

The number of mature oocytes oviposited from park^P23, eIF-4E⁰⁷²³⁸/park^P23 mutant females mated to wild-type males is 42.8 oocytes/mother, and the rate of larval hatching is 40.5%, much improved compared to park^P23 homozygous mutant females.

eIF-4E⁰⁷²³⁸/+ significantly abrogates the oogenesis defects in park^P23/park^P23 mutant females. Most egg chambers appear normal in these animals, and the reduced size of park^P23/park^P23 ovaries is rescued in a eIF-4E⁰⁷²³⁸/+ background. Oogenesis defects are not restored in Thor^Δ1034-2617/Thor^Δ1034-2617; park^P23, eIF-4E⁰⁷²³⁸/park^P23 mutant animals.

(Ottone et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

park^P23 is rescued by park^+tR

(Ottone et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

park^P23

Name Synonyms

parkinP23

(Ottone et al., 2011)

Secondary FlyBase IDs

References (2)

Report Sections

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