FlyBase Allele Report: Dmel\for[P1.UAS]

General Information

Symbol

Dmel\for^P1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0159222

Feature type

allele

Associated gene

Dmel\for

Associated Insertion(s)

Carried in Construct

P{UAS-for.P1}

Key Links

Transgenic product class

wild_type

(MacPherson et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(MacPherson et al., 2004)

Mutagen

in vitro construct

(MacPherson et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-for.P1}

Cytology

Description

UASt regulatory sequences drive expression of the for P1 isoform.

(MacPherson et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

for

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Kanao et al., 2012)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Kanao et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 have a reduction in lifespan compared to controls and show mild defects in climbing ability.

(Kanao et al., 2012)

The basal rate of fluid transport in Malpighian tubules expressing for^P1.Scer\UAS under the control of Scer\GAL4^c42 is not significantly different from that of control Malpighian tubules. However, upon stimulation by cGMP, Malpighian tubules expressing for^P1.Scer\UAS under the control of Scer\GAL4^c42 show significantly elevated fluid transport rates compared to control Malpighian tubules. Upon stimulation by capa protein, Malpighian tubules expressing for^P1.Scer\UAS under the control of Scer\GAL4^c42 do not show a significantly different fluid transport rate than control Malpighian tubules.

(MacPherson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has visible phenotype, enhanceable by Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has abnormal neuroanatomy | adult stage | RU486 conditional phenotype, enhanceable | RU486 conditional by Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has abnormal locomotor behavior | adult stage | RU486 conditional phenotype, enhanceable | RU486 conditional by Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}

(Kanao et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS has short lived | RU486 conditional phenotype, suppressible | partially by foxo²¹/foxo[+]

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS has abnormal locomotor behavior | adult stage | RU486 conditional phenotype, suppressible | partially by foxo²¹/foxo[+]

(Kanao et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has visible phenotype, non-suppressible by Lrrk^3KD.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2012)

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has visible phenotype, non-suppressible by Lrrk^e03680/Lrrk^e03680

(Kanao et al., 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has visible phenotype

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has short lived | RU486 conditional phenotype

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has abnormal neuroanatomy | adult stage | RU486 conditional phenotype

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has abnormal locomotor behavior | adult stage | RU486 conditional phenotype

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^S259A.UAS has short lived | RU486 conditional phenotype

(Kanao et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has eye phenotype, enhanceable by Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has eye | RU486 conditional phenotype, enhanceable | RU486 conditional by Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}

(Kanao et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has eye phenotype, non-suppressible by Lrrk^3KD.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2012)

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has eye phenotype, non-suppressible by Lrrk^e03680/Lrrk^e03680

(Kanao et al., 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa has eye phenotype

(Kanao et al., 2012)

Scer\GAL4^{elav.Switch.PO}, for^P1.UAS, foxo^UAS.cPa has dopaminergic neuron | RU486 conditional phenotype

(Kanao et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^GMR.PF results in degeneration of the eye. The severity of this phenotype is enhanced by co-expression of Lrrk^{I1915T.Scer\UAS} but is not suppressed by either co-expression of Lrrk^3KD.Scer\UAS or by Lrrk^e03680/Lrrk^e03680.

Co-expression of for^P1.Scer\UAS and Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in flies with normal eyes.

Co-expression of foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 results in loss of dopaminergic neurons. This phenotype is enhanced by co-expression of Lrrk^{I1915T.Scer\UAS}.

Co-expression of foxo^{S259A.Scer\UAS} and for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 does not affect the number of dopaminergic neurons.

Co-expression of foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 results in flies with reduced climbing ability. This phenotype is worsened by co-expression of Lrrk^{I1915T.Scer\UAS}.

Flies co-expressing foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 have a reduced lifespan compared to controls.

Flies co-expressing foxo^{S259A.Scer\UAS} and for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 have a reduced lifespan compared to controls.

The defect in climbing ability and reduction in lifespan seen in flies expressing for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 is partially suppressed by foxo²¹/+.

(Kanao et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

for^P1.Scer\UAS

for^P1.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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