FlyBase Allele Report: Dmel\spdo[UAS.cOa]

General Information

Symbol

Dmel\spdo^UAS.cOa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of O'Conner-Giles

FlyBase ID

FBal0175459

Feature type

allele

Associated gene

Dmel\spdo

Associated Insertion(s)

Carried in Construct

P{UAS-spdo.O}

Also Known As

UAS-spdo

Key Links

Transgenic product class

cDNA

(O'Connor-Giles and Skeath, 2003)

wild_type

(O'Connor-Giles and Skeath, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(O'Connor-Giles and Skeath, 2003)

wild_type

(O'Connor-Giles and Skeath, 2003)

Mutagen

in vitro construct

(Skeath, 2007.3.20, O'Connor-Giles and Skeath, 2003)

Progenitor genotype

Carried in construct

P{UAS-spdo.O}

Cytology

Description

UASt sequences drive expression of the spdo ORF from ESTs RE04681 and RE23355 (2 combined to give one complete ORF because RE23355 contains an apparent missense mutation at aa 289 and RE04681 is incompletely spliced).

(O'Connor-Giles and Skeath, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

spdo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size

(Babaoglan et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^sca-109-68 induces supernumerary thoracic sensory bristles, as compared to controls.

(Shukla et al., 2017)

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^ptc-559.1 promotes ectopic scutellar bristle formation.

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^ptc-559.1 promotes the formation of ectopic sensory organ precursors in late third instar wing imaginal discs.

Expression of spdo^Scer\UAS.cOa in the wing, under the control of Scer\GAL4^nub-AC-62, results in wing notching, vein thickening and reduced wing size.

Expression of spdo^Scer\UAS.cOa in the embryonic mesoderm, under the control of Scer\GAL4^twi.PG, promotes the formation of extra svp-positive heart precursors by stage 12, and extra progenitor svp-positive pericardial cells and cardioblasts by stage 16. Extra svp-negative cardioblasts also develop.

(Babaoglan et al., 2009)

Within somatic clones of Scer\GAL4^Act5C.PI cells in a spdo^Scer\UAS.cOa background, development of notal sensory clusters appears to occur normally in pupae and no defects are seen in notal bristles in adults.

(Jafar-Nejad et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has abnormal size phenotype, enhanceable by Df(1)N-81k1/+

(Babaoglan et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has abnormal size phenotype, suppressible by Df(1)N-81k1/numb[+]/numb²/+

(Babaoglan et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sca-109-68, spdo^UAS.cOa has mechanosensory chaeta | adult stage | increased number phenotype, enhanceable by Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68

(Shukla et al., 2017)

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has scutellar bristle | ectopic phenotype, enhanceable by Df(1)N-81k1/+

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing phenotype, enhanceable by Df(1)N-81k1/+

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing vein phenotype, enhanceable by Df(1)N-81k1/+

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing margin phenotype, enhanceable by Df(1)N-81k1/+

(Babaoglan et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has scutellar bristle | ectopic phenotype, suppressible | partially by numb^RNAi.UAS.cTa/Scer\GAL4^ptc-559.1

(Babaoglan et al., 2009)

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has scutellar bristle | ectopic phenotype, suppressible | partially by numb[+]/numb¹

(Babaoglan et al., 2009)

Scer\GAL4^ptc-559.1, spdo^UAS.cOa has scutellar bristle | ectopic phenotype, suppressible | partially by numb[+]/numb²

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing phenotype, suppressible | partially by Df(1)N-81k1/numb[+]/numb²/+

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing vein phenotype, suppressible | partially by Df(1)N-81k1/numb[+]/numb²/+

(Babaoglan et al., 2009)

Scer\GAL4^nub-AC-62, spdo^UAS.cOa has wing margin phenotype, suppressible | partially by Df(1)N-81k1/numb[+]/numb²/+

(Babaoglan et al., 2009)

Scer\GAL4^twi.PG, spdo^UAS.cOa has embryonic heart cardioblast | increased number | embryonic stage 16 phenotype, suppressible by numb[+]/numb²

(Babaoglan et al., 2009)

Scer\GAL4^twi.PG, spdo^UAS.cOa has embryonic pericardial cell | increased number | embryonic stage 16 phenotype, suppressible by numb[+]/numb²

(Babaoglan et al., 2009)

Scer\GAL4^twi.PG, spdo^UAS.cOa has embryonic heart cardioblast | increased number | embryonic stage 16 phenotype, suppressible by numb²

(Babaoglan et al., 2009)

Scer\GAL4^twi.PG, spdo^UAS.cOa has embryonic pericardial cell | increased number | embryonic stage 16 phenotype, suppressible by numb²

(Babaoglan et al., 2009)

Enhancer of

Statement

Reference

spdo^UAS.cOa, Scer\GAL4^sca-109-68 is an enhancer of mechanosensory chaeta | adult stage | increased number phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68

(Shukla et al., 2017)

Scer\GAL4^twi.PG/spdo^UAS.cOa is an enhancer of embryonic heart cardioblast | embryonic stage 16 phenotype of numb²

(Babaoglan et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^twi.PG/spdo^UAS.cOa is a suppressor of embryonic pericardial cell | ectopic | embryonic stage 16 phenotype of numb²

(Babaoglan et al., 2009)

Other

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^sca-109-68, spdo^UAS.cOa has external sensory organ | adult stage phenotype

(Shukla et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of both spdo^Scer\UAS.cOa and Rbfox1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-109-68 strongly enhances the supernumerary thoracic sensory bristle phenotype observed upon the expression of either spdo^Scer\UAS.cOa alone or Rbfox1^{dsRNA.Scer\UAS} alone, and even leads to an increase in the number of other (i.e. non-bristle) sensory organs in the thorax, as compared to controls.

(Shukla et al., 2017)

Ectopic scutellar bristle formation in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^ptc-559.1 is dramatically enhanced in a Df(1)N-81k1/+ background.

Ectopic scutellar bristle formation in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^ptc-559.1 is suppressed by co-expression of numb^{dsRNA.Scer\UAS.T:Hsap\MYC}.

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 results in dramatically enhanced wing notching, vein thickening, and reduced wing size in a Df(1)N-81k1/+ background.

Simultaneously expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a Df(1)N-81k1/+ and numb²/+ background partially suppresses wing notching, vein thickening, and reduced wing size observed in the absence of numb².

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a numb¹/+ background.

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a numb²/+ background.

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG in the presence of numb²/+ suppresses the formation of extra svp-positive heart precursors by stage 12, and extra progenitor svp-positive pericardial cells, to almost wildtype levels.

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG in a numb² mutant embryo blocks the formation of svp-positive precursor cells, resulting in dramatically fewer svp-positive heart cells and less cardioblasts. Fewer svp-negative cardioblasts develop.

eve-positive equivalence groups form but less eve-positive pericardial cells are seen compared to wildtype when spdo^Scer\UAS.cOa is expressed under the control of Scer\GAL4^twi.PG in a numb² background.

eve-positive equivalence groups form normally in numb² mutant embryos that express spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG. However, eve-positive precursors fail to segregate from approximately half of the equivalence groups, resulting in the formation of far fewer eve-positive pericardial cells than in numb² mutant embryos.

(Babaoglan et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spdo^UAS.cOa is rescued by Scer\GAL4^twi.PG/spdo^G104

(Babaoglan et al., 2009)

Partially rescues

Scer\GAL4^sca-109-68/spdo^UAS.cOa partially rescues spdo^G104

(O'Connor-Giles and Skeath, 2003)

Comments

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG in a spdo^G104 mutant background rescues the loss of eve-positive pericardial cell numbers observed in spdo^G104 mutant embryos.

(Babaoglan et al., 2009)

spdo^Scer\UAS.cOa; Scer\GAL4^sca-109-68 completely or almost completely rescues formation of U2 neurons and suppresses RP2 neuron duplication in spdo^G104 homozygous embryos.

(O'Connor-Giles and Skeath, 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9934

w^*; P{UAS-spdo.O}G14

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

spdo^G104

(Li et al., 2016)

spdo^Scer\UAS.cOa

spdo^UAS.cOa

Name Synonyms

Saccharomyces cerevisiae UAS construct of O'Conner-Giles

(O'Connor-Giles and Skeath, 2003)

Secondary FlyBase IDs

References (9)

Report Sections

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