FB2026_02 , released June 18, 2026
Allele: Dmel\nerfin-154
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General Information
Symbol
Dmel\nerfin-154
Species
D. melanogaster
Name
FlyBase ID
FBal0175567
Feature type
allele
Associated gene
Dmel\nerfin-1
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Kuzin et al., 2005)
FLPase
(Kuzin et al., 2005)
I-SceI
(Kuzin et al., 2005)
ends-in gene targeting
(Kuzin et al., 2005)
Progenitor genotype
Cytology
Description

A deletion removes a 2347bp segment that includes the minimal promoter and 5' transcribed leader sequence.

(Kuzin et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In mutant embryos, most embryos within longitudinal connectives fail to project across segmental boundaries and frequently terminate in tangles at or near the boundaries. In addition, both the anterior and posterior commissures of each segment fail to develop properly. Also the overall fascicle organisation and apparent axon density of the ventral commissures is affected. The motor neuron nerve tracts and the axon patterning of the peripheral nervous system appears normal. In mutant embryos the pCC interneurons fail to send their axons anteriorly and instead project their axons either in a lateral or posterior direction, and any of the posterior projecting pCC axons cross the ventral midline in adjacent posterior segments. By stage 14, all pCC interneurons have misguided axons and many of these axons have extensive side branches. The overall development and patterning of the aCC and RP2 motorneuron axon tracts do not appear to be adversely affected.

      (Kuzin et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      nerfin-154 has larval longitudinal connective phenotype, enhanceable by prosIl3

      (Kuzin et al., 2005)
      Enhancer of
      Statement
      Reference

      nerfin-154 is an enhancer of larval longitudinal connective phenotype of prosIl3

      (Kuzin et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      nerfin-154 is rescued by nerfin-1+t11.154

      (Kuzin et al., 2005)
      Partially rescued by

      nerfin-154 is partially rescued by Scer\GAL4elav-C155/nerfin-1UAS.cKa

      (Kuzin et al., 2005)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Created as a consequence of the "ends in" homologous recombination gene knockout technique. The deletion was most likely caused by exonuclease digestion of the targeting vector after the SceI exonuclease induced double strand break but before its integration into the nerfin-1 chromosomal locus. The deletion was detected after the allelic substitution step and are most likely the result of illegitimate recombination between micro-homologies present in the minimal promoter of one copy of the nerfin-1 duplication and the transcribed region of the tandem copy.

      (Kuzin et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Df(3L)nerfin-154
      (Kuzin et al., 2005)
      nerfin-154
      (Guo et al., 2019, Duan et al., 2018)
      nerfin-1null
      (Kuzin et al., 2005)
      nerfin-1Δ54
      (Kavaler et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)