FlyBase Allele Report: Dmel\nerfin-1[159]

General Information

Symbol

Dmel\nerfin-1¹⁵⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0175568

Feature type

allele

Associated gene

Dmel\nerfin-1

Associated Insertion(s)

Carried in Construct

Also Known As

Df(3L)nerfin-1¹⁵⁹

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Kuzin et al., 2005)

FLPase

(Kuzin et al., 2005)

I-SceI

(Kuzin et al., 2005)

ends-in gene targeting

(Kuzin et al., 2005)

Progenitor genotype

Cytology

Description

Two deletions remove a 1314bp and a 593bp segment that includes the minimal promoter and 5' transcribed leader sequence.

(Kuzin et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Narbonne-Reveau et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | somatic clone

(Xu et al., 2017)

abnormal neuroanatomy | late embryonic stage (with Df(3L)Exel6086)

(Stratmann et al., 2019)

increased occurrence of cell division | larval stage | somatic clone

(Xu et al., 2017)

neoplasia | adult stage | somatic clone

(Xu et al., 2017)

Phenotype Manifest In

adult optic lobe | larval stage | somatic clone

(Xu et al., 2017)

apterous-expressing neuron of the lateral cluster of the thorax | late embryonic stage (with Df(3L)Exel6086)

(Stratmann et al., 2019)

neuroblast | ectopic | larval stage | somatic clone

(Xu et al., 2017)

neuron projection bundle of larval ventral nerve cord | late embryonic stage (with Df(3L)Exel6086)

(Stratmann et al., 2019)

Detailed Description

Statement

Reference

nerfin-1¹⁵⁹/Df(3L)Exel6086 embryos show strongly disorganized Fas2-immunopositive fascicles and disorganized or absent ap-expressing axons, despite having a similar number of ap-expressing neurons as controls.

(Stratmann et al., 2019)

Larval optic lobe nerfin-1¹⁵⁹/nerfin-1¹⁵⁹ mutant clones include ectopic neuroblasts, located where post-mitotic neurons should be. These neuroblasts exhibit proliferation potential as shown by an increase in the number of mitotic cells in these clones, and form tumors at the adult stage. Medulla neurons are generated normally, but soon begin to de-differentiate, suggesting that the origin of these ectopic neuroblasts is de-differentiation of neurons. The number of GMCs does not significantly change in nerfin-1¹⁵⁹/nerfin-1¹⁵⁹ mutant clones.

(Xu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nerfin-1¹⁵⁹ has neoplasia | somatic clone | adult stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/N^KK102890

(Xu et al., 2017)

nerfin-1¹⁵⁹ has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/N^KK102890

(Xu et al., 2017)

nerfin-1¹⁵⁹ has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/Delta^GL00520

(Xu et al., 2017)

NOT suppressed by

Statement

Reference

nerfin-1¹⁵⁹ has abnormal neuroanatomy | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Myc^KK103869/Scer\GAL4^Tub.PU

(Xu et al., 2017)

nerfin-1¹⁵⁹ has abnormal neuroanatomy | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Tub.PU/mTor^TED.UAS

(Xu et al., 2017)

Suppressor of

Statement

Reference

nerfin-1¹⁵⁹/nerfin-1[+] is a suppressor | partially | somatic clone of abnormal neuroanatomy | somatic clone | adult stage phenotype of pros^IG2227

(Hartl et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

nerfin-1¹⁵⁹ has neuroblast | ectopic | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/N^KK102890

(Xu et al., 2017)

nerfin-1¹⁵⁹ has adult optic lobe | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/N^KK102890

(Xu et al., 2017)

nerfin-1¹⁵⁹ has neuroblast | ectopic | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/Delta^GL00520

(Xu et al., 2017)

nerfin-1¹⁵⁹ has adult optic lobe | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Tub.PU/Delta^GL00520

(Xu et al., 2017)

NOT suppressed by

Statement

Reference

nerfin-1¹⁵⁹ has neuroblast | ectopic | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Myc^KK103869/Scer\GAL4^Tub.PU

(Xu et al., 2017)

nerfin-1¹⁵⁹ has adult optic lobe | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Myc^KK103869/Scer\GAL4^Tub.PU

(Xu et al., 2017)

nerfin-1¹⁵⁹ has neuroblast | ectopic | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Tub.PU/mTor^TED.UAS

(Xu et al., 2017)

nerfin-1¹⁵⁹ has adult optic lobe | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Tub.PU/mTor^TED.UAS

(Xu et al., 2017)

Suppressor of

Statement

Reference

nerfin-1¹⁵⁹/nerfin-1[+] is a suppressor | partially | somatic clone of carbon dioxide sensitive neuron | ectopic | somatic clone phenotype of pros^IG2227

(Hartl et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Clonal expression of N^KK102890 or Dl^GL00520 (but not Myc^KK103869 or Tor^TED.Scer\UAS) under the control of Scer\GAL4^tub.PU, largely suppresses the formation of ectopic neuroblasts in larval optic lobe nerfin-1¹⁵⁹/nerfin-1¹⁵⁹ mutant clones; and expression of N^KK102890 suppresses tumor formation at the adult stage in these clones.

(Xu et al., 2017)

The number of ectopic CO[[2]] neurons on the maxillary palp in animals containing pros^IG2227 clones is significantly suppressed if they are also carrying nerfin-1¹⁵⁹/+.

(Hartl et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nerfin-1¹⁵⁹ is rescued by Scer\GAL4^Tub.PU/nerfin-1^UAS.cXa

(Xu et al., 2017)

Partially rescued by

nerfin-1¹⁵⁹ is partially rescued by Scer\GAL4^Tub.PU/nerfin-1^dZF3.UAS

(Xu et al., 2017)

Not rescued by

nerfin-1¹⁵⁹ is not rescued by nerfin-1^dZF1.UAS/Scer\GAL4^Tub.PU

(Xu et al., 2017)

nerfin-1¹⁵⁹ is not rescued by Scer\GAL4^Tub.PU/nerfin-1^dZF2.UAS

(Xu et al., 2017)

Comments

Expression of nerfin-1^{dZF3.Scer\UAS} or nerfin-1^Scer\UAS.cXa (but not nerfin-1^{dZF1.Scer\UAS} or nerfin-1^{dZF2.Scer\UAS}) under the control of Scer\GAL4^tub.PU substantially decreases the number of ectopic neuroblasts in nerfin-1¹⁵⁹/nerfin-1¹⁵⁹ mutant clones.

(Xu et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Created as a consequence of the "ends in" homologous recombination gene knockout technique. The deletion was most likely caused by exonuclease digestion of the targeting vector after the SceI exonuclease induced double strand break but before its integration into the nerfin-1 chromosomal locus. The deletion was detected after the allelic substitution step and are most likely the result of illegitimate recombination between micro-homologies present in the minimal promoter of one copy of the nerfin-1 duplication and the transcribed region of the tandem copy.

(Kuzin et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Df(3L)nerfin-1¹⁵⁹

(Narbonne-Reveau et al., 2025, Narbonne-Reveau et al., 2016, Kuzin et al., 2005)

nerfin-1¹⁵⁹

(Vissers et al., 2018, Xu et al., 2017)

nerfin-1^D159

(Stratmann et al., 2019)

nerfin-1^null

(Kuzin et al., 2005)

nerfin¹⁵⁹

(Hartl et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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