Open Close
General Information
Symbol
Dmel\nerfin-1159
Species
D. melanogaster
Name
FlyBase ID
FBal0175568
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Df(3L)nerfin-1159
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Two deletions remove a 1314bp and a 593bp segment that includes the minimal promoter and 5' transcribed leader sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

nerfin-1159/Df(3L)Exel6086 embryos show strongly disorganized Fas2-immunopositive fascicles and disorganized or absent ap-expressing axons, despite having a similar number of ap-expressing neurons as controls.

Larval optic lobe nerfin-1159/nerfin-1159 mutant clones include ectopic neuroblasts, located where post-mitotic neurons should be. These neuroblasts exhibit proliferation potential as shown by an increase in the number of mitotic cells in these clones, and form tumors at the adult stage. Medulla neurons are generated normally, but soon begin to de-differentiate, suggesting that the origin of these ectopic neuroblasts is de-differentiation of neurons. The number of GMCs does not significantly change in nerfin-1159/nerfin-1159 mutant clones.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Clonal expression of NKK102890 or DlGL00520 (but not MycKK103869 or TorTED.Scer\UAS) under the control of Scer\GAL4tub.PU, largely suppresses the formation of ectopic neuroblasts in larval optic lobe nerfin-1159/nerfin-1159 mutant clones; and expression of NKK102890 suppresses tumor formation at the adult stage in these clones.

The number of ectopic CO[[2]] neurons on the maxillary palp in animals containing prosIG2227 clones is significantly suppressed if they are also carrying nerfin-1159/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Created as a consequence of the "ends in" homologous recombination gene knockout technique. The deletion was most likely caused by exonuclease digestion of the targeting vector after the SceI exonuclease induced double strand break but before its integration into the nerfin-1 chromosomal locus. The deletion was detected after the allelic substitution step and are most likely the result of illegitimate recombination between micro-homologies present in the minimal promoter of one copy of the nerfin-1 duplication and the transcribed region of the tandem copy.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)