FlyBase Allele Report: Dmel\mtrm[126]

General Information

Symbol

Dmel\mtrm¹²⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0175579

Feature type

allele

Associated gene

Dmel\mtrm

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Xiang et al., 2005)

Mutagen

P-element activity

(Xiang et al., 2007, Xiang et al., 2005)

Progenitor genotype

P{SUPor-P}Exo70^KG08051

(Xiang et al., 2007)

Cytology

Description

Imprecise excision of the progenitor insertion, removing 80bp of 5'UTR and 123bp of coding sequence (deleting the first 41 amino acids).

(Xiang et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:8,412,995..8,413,198 [+]

Comment:

203 bp deletion resulting from imprecise of excision of P{SUPor-P}Exo70^KG08051 that takes out 80bp upstream of the translation start codon and extends 123bp downstream (deleting the first 41 amino acids).

(Xiang et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | dominant

(Xiang et al., 2007, Xiang et al., 2005)

female sterile (with Df(3L)66C-T2-10)

(Bonner et al., 2013)

female sterile | recessive

(Xiang et al., 2005)

male fertile

(Xiang et al., 2007)

Phenotype Manifest In

cystoblast

(Xiang et al., 2005)

karyosome

(Xiang et al., 2007)

meiotic spindle (with Df(3L)66C-T2-10)

(Bonner et al., 2013)

oocyte & nuclear chromosome

(Xiang et al., 2005)

oocyte nucleus (with Df(3L)66C-T2-10)

(Bonner et al., 2013)

oocyte nucleus | oogenesis stage S11

(Xiang et al., 2007)

oocyte nucleus | oogenesis stage S12

(Xiang et al., 2007)

Detailed Description

Statement

Reference

mtrm¹²⁶/Df(3L)66C-T2-10 oocyte nuclei are severely abnormal in both prometaphase I and metaphase I. Intact chromosomes can be seen in some oocytes, but they are frequently not associated into a single chromosome mass or a single spindle and in many oocytes the nucleus appears severely fragmented. Spindles are either absent or abnormal looking. In 65% of oocytes with fully developed dorsal appendages, a discernible oocyte nucleus can not be identified.

(Bonner et al., 2013)

Heterozygosity for mtrm¹²⁶ results in elevated achiasmate X (42%) and 4th (37%) chromosome nondisjunction in In(1)FM7/X females compared to controls (the In(1)FM7 chromosome is present to fully suppress X chromosomal exchange).

42% of stage 12 oocytes show nuclear envelope breakdown (NEB) in mtrm¹²⁶/+ females (NEB does not occurs until stage 13 in wild-type egg oocytes). 60% of stage 11 oocytes and 97% of stage 12 oocytes show NEB in homozygous females. 26% of heterozygous oocytes observed within 20 minutes before NEB have a disordered karyosome.

The karyosome usually dissolves within 10-20 minutes after NEB in In(1)FM7/X ; mtrm¹²⁶/+ oocytes, and the individual bivalents become clearly visible (in 88% of oocytes examined, the chromosomes are individualised during spindle assembly). This contrasts with control oocytes, where the karyosome remains associated after NEB and the chiasmate chromosomes are still condensed in a single mass at metaphase I. However, most of the mutant oocytes that undergo bivalent individualisation eventually form bipolar spindles with chiasmate chromosomes properly balanced on the metaphase plate.

43% of oocyte nuclei show an aberrant co-orientation of 4th chromosome centromeres to the same pole, while 6% of these oocytes have aberrant X centromere co-orientations in mtrm¹²⁶/+ females with two normal sequence X chromosomes. In(1)FM7/X mtrm¹²⁶/+ females show 43% abnormal centromere co-orientation for the X chromosome and 37% for the 4th chromosome.

(Xiang et al., 2007)

mtrm¹²⁶ homozygotes undergo an extra round of cystoblast divisions prior to the onset of meiosis. Stage 13-14 oocytes from these females have improperly condensed chromosomes.

(Xiang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mtrm¹²⁶ has abnormal meiotic cell cycle | dominant phenotype, suppressible by polo[+]/polo^KG03033

(Xiang et al., 2007)

mtrm¹²⁶ has abnormal meiotic cell cycle | dominant phenotype, suppressible by polo^16-1/polo[+]

(Xiang et al., 2007)

mtrm¹²⁶ has abnormal meiotic cell cycle | dominant phenotype, suppressible by Df(3L)rdgC-co2/+

(Xiang et al., 2007)

Suppressor of

Statement

Reference

mtrm[+]/mtrm¹²⁶ is a suppressor | maternal effect | partially of abnormal meiotic cell cycle | maternal effect phenotype of grau^RM61/grau^QQ36

(Whitfield et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

mtrm¹²⁶ has oocyte nucleus | oogenesis stage S12 phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/polo^UASp.cXa

(Xiang et al., 2007)

Suppressed by

Statement

Reference

mtrm¹²⁶ has oocyte nucleus | oogenesis stage S12 phenotype, suppressible | partially by polo^16-1/polo[+]

(Xiang et al., 2007)

mtrm¹²⁶ has karyosome phenotype, suppressible by polo^16-1/polo[+]

(Xiang et al., 2007)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, mtrm¹²⁶, polo^UASp.cXa has oocyte nucleus | oogenesis stage S11 phenotype

(Xiang et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The meiotic arrest phenotype seen in eggs laid by grau^QQ36/grau^RM61 females is partially suppressed if the females also carry mtrm¹²⁶/+.

(Whitfield et al., 2013)

polo^KG03033/+, polo^16-1/+ and Df(3L)rdgC-co2/+ each suppress the elevated levels of achiasmate X and 4th chromosome nondisjunction seen in In(1)FM7/X mtrm¹²⁶/+ females.

The precocious nuclear envelope breakdown (NEB) seen in stage 12 oocytes of mtrm¹²⁶/+ females is strongly suppressed by polo^16-1/+. The defects in karyosome morphology seen in mtrm¹²⁶/+ oocytes observed within 20 minutes of NEB are also largely suppressed by polo^16-1/+.

The precocious NEB seen in oocytes of mtrm¹²⁶/+ females is enhanced by expression of polo^{Scer\UAS.P\T.cXa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}; the frequency of NEB in stage 12 oocytes is increased to 77%, and NEB is seen in 19% of stage 11 oocytes.

polo^16-1/+ largely suppresses the karyosome maintenance defects seen in mtrm¹²⁶/+ oocytes. polo^16-1/+ fully suppresses the defect in X and 4th chromosome centromere co-orientation that is seen in mtrm¹²⁶/+ females.

(Xiang et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mtrm¹²⁶ is rescued by Scer\GAL4^{VP16.nanos.UTR}/mtrm^S124A.UASp

(Xiang et al., 2007)

Not rescued by

mtrm¹²⁶ is not rescued by mtrm^T40A.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Xiang et al., 2007)

mtrm¹²⁶ is not rescued by Scer\GAL4^{VP16.nanos.UTR}/mtrm^{T40A.S124A.UASp}

(Xiang et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mtrm¹²⁶

(Bonner and Hawley, 2019, Hanlon et al., 2018, Bauerly et al., 2014, Bonner et al., 2013, Whitfield et al., 2013, Xiang et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close