FlyBase Allele Report: Dmel\ds[D36]

General Information

Symbol

Dmel\ds^D36

Species

D. melanogaster

Name

FlyBase ID

FBal0175705

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

Also Known As

ds^36D

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rodriguez, 2018.3.27, Rodriguez, 2004)

Progenitor genotype

P{PZ}ds⁰⁵¹⁴²

(Rodriguez, 2018.3.27, Rodriguez, 2004)

Cytology

Description

Deletion of 400bp in the second intron. Generated by imprecise excision of P{PZ}ds⁰⁵¹⁴².

(Rodriguez, 2018.3.27)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | somatic clone (with ds^UAO71)

(Baena-Lopez et al., 2005, Baena-Lopez et al., 2005)

abnormal cell shape | somatic clone

(Baena-Lopez et al., 2005)

abnormal cell shape | somatic clone (with ds^UAO71)

(Baena-Lopez et al., 2005)

abnormal planar polarity | adult stage (with ds^UAO71)

(Tripathi and Irvine, 2024)

abnormal size | adult stage (with ds^38k)

(Revilla-Yates et al., 2015)

abnormal size | adult stage (with ds^UAO71)

(Baena-Lopez et al., 2005)

abnormal size | third instar larval stage

(Revilla-Yates et al., 2015)

decreased size | adult stage (with ds^UAO71)

(Tripathi and Irvine, 2024)

hyperplasia

(Ziosi et al., 2010)

majority die during P-stage (with ds^38k), with Scer\GAL4^hh.PU, ds^UAS.cTa

(Revilla-Yates et al., 2015)

visible (with ds^38k)

(Revilla-Yates et al., 2015)

visible | adult stage (with ds^UAO71)

(Tripathi and Irvine, 2024)

Phenotype Manifest In

eye (with ds^UAO71)

(Baena-Lopez et al., 2005)

eye disc | somatic clone (with ds^UAO71)

(Baena-Lopez et al., 2005)

fat body | third instar larval stage

(Revilla-Yates et al., 2015)

leg (with ds^UAO71)

(Tripathi and Irvine, 2024, Baena-Lopez et al., 2005)

mitochondrion | third instar larval stage

(Revilla-Yates et al., 2015)

segment of leg | decreased number (with ds^UAO71)

(Tripathi and Irvine, 2024)

wing (with ds^38k)

(Revilla-Yates et al., 2015)

wing (with ds^38k), with Scer\GAL4^hh.PU, ds^UAS.cTa

(Revilla-Yates et al., 2015)

wing (with ds^UAO71)

(Tripathi and Irvine, 2024, Baena-Lopez et al., 2005)

wing disc | somatic clone (with ds^UAO71)

(Baena-Lopez et al., 2005, Baena-Lopez et al., 2005)

wing hair (with ds^38k)

(Revilla-Yates et al., 2015)

wing vein (with ds^38k)

(Revilla-Yates et al., 2015)

Detailed Description

Statement

Reference

The wings of ds^38k/ds^36D transheterozygotes are smaller and rounder than in controls, and have vein and hair patterning defects.

(Revilla-Yates et al., 2015)

ds^36D clones show a rounded shape than wild-type clones when generated in the wing disc. Postmitotic orientation is randomized in ds^36D/ds^UAO71 clones in the wing disc.

(Baena-Lopez et al., 2005)

The orientation of cell division is randomized in ds^36D/ds^UAO71 mitotic clones in wing discs. Therefore, in contrast to wild-type postmitotic cells, ds^36D/ds^UAO71 postmitotic cells do not show any preferential orientation. Mitotic recombination clones in ds^36D/ds^UAO71 mutant wings show a rounded shape, instead of the elongated shape seen in wild-type clones. At the adult stage, ds^36D/ds^UAO71 wings are shorter, rounder and wider than wild type. Likewise, ds^36D/ds^UAO71 mitotic clones in eye discs show a defective shape and preferential orientations of cell divisions are lost. Resulting adult eyes are rounder than wild type. ds^36D/ds^UAO71 flies also have shorter, wider legs than wild-type flies.

(Baena-Lopez et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ds^D36 has hyperplasia phenotype, enhanceable by Myc^UAS.cZa/Scer\GAL4^hh.PU

(Ziosi et al., 2010)

Other

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^hh.PU, ds^D36 has increased cell size phenotype

(Ziosi et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

dm^Scer\UAS.cZa overexpression clones (under the control of Scer\GAL4^hh.PU) found in the posterior of the wing disc strongly enhance the proliferative activity of ds^36D mutant cells. In addition these cells are larger in dm^Scer\UAS.cZa clones than in a wild-type background.

(Ziosi et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Not rescued by

ds^38k/ds^D36 is not rescued by ds^{intra.UAS.Tag:V5}

(Revilla-Yates et al., 2015)

ds^38k/ds^D36 is not rescued by ds^UAS.cTa

(Revilla-Yates et al., 2015)

ds^38k/ds^D36 is not rescued by ds^ex.UAS.GFP

(Revilla-Yates et al., 2015)

Comments

Instead of rescuing the wing defects of ds^38k/ds^36D transheterozygotes, the Scer\GAL4^hh.PU-driven expression of either ds^Scer\UAS.cTa or ds^{intra.Scer\UAS.T:SV5\V5} leads to high lethality during early pupal stages and even enhances the wing defects in surviving adults.

The expression of ds^{ex.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^hh.PU does not rescue the wing defects observed in ds^38k/ds^36D transheterozygotes.

(Revilla-Yates et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ds^36D

(Tripathi and Irvine, 2024, Revilla-Yates et al., 2015, Ambegaonkar et al., 2012, Ambegaonkar et al., 2012, Feng and Irvine, 2009, Mao et al., 2006)

ds^D36

(Ziosi et al., 2010, Baena-Lopez et al., 2005, Baena-Lopez et al., 2005, Rodriguez, 2004)

Name Synonyms

Secondary FlyBase IDs

References (11)

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