FlyBase Allele Report: Dmel\Src42A[YF.UAS]

General Information

Symbol

Dmel\Src42A^YF.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175797

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

P{UAS-Src42A.YF}

Key Links

Transgenic product class

missense_variant

(Pedraza et al., 2004)

polypeptide_post_translational_processing_variant

(Pedraza et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Pedraza et al., 2004)

polypeptide_post_translational_processing_variant

(Pedraza et al., 2004)

Mutagen

in vitro construct

(Pedraza et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Src42A.YF}

Cytology

Description

UASt sequences drive expression of a constitutively active form of Src42A.

(Pedraza et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Src42A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

increased cell death, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

lethal, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Phenotype Manifest In

embryonic leading edge cell, with Scer\GAL4^pnr-MD237

(Takahashi et al., 2005)

eye, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

eye disc | larval stage, with Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Detailed Description

Statement

Reference

Expression of Src42A^YF.Scer\UAS, driven by Scer\GAL4^pnr-MD237, prevents dorsal epithelial cells from elongating normally. Occasionally, Src42A^YF.Scer\UAS-overexpressing cells become separated from the amnioserosa or dorsal plane.

(Takahashi et al., 2005)

When Src42A^YF.Scer\UAS is driven by Scer\GAL4^GMR.PF, a range of phenotypes is seen in the eye in an insertion, dose dependent and temperature sensitive manner. These range through lethality, a complete loss of the eye, to small rough eyes. When the developing eye disc is examined, ectopic proliferation of uncommitted cells (which usually arrest in G₁/G₀) in the disc is seen. Mutant eye discs also exhibit increased cell death, displaying a stronger effect than Src42A^Scer\UAS.cPa.

(Pedraza et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^YF.UAS has increased cell death phenotype, non-suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Scer\GAL4^GMR.PF, Src42A^YF.UAS has increased occurrence of cell division | larval stage phenotype, non-suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^Mhc.PW, Src42A^YF.UAS has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^YF.UAS has eye phenotype, non-suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^Mhc.PW, Src42A^YF.UAS has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae with expression of Src42A^YF.Scer\UAS driven in muscles by Scer\GAL4^Mhc.PW (at 18[o]C) show significant decreases in mEPSP (decreased quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; when expression is driven by Scer\GAL4^VGlut-OK371 the significant increase in quantal content is still seen.

(Spring et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Src42A^YF.Scer\UAS

Src42A^YF.UAS

Src42A^YF

(Takahashi et al., 2005)

src42

(Pedraza et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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