FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ced-12RNAi.UAS
Open Close
General Information
Symbol
Dmel\Ced-12RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0176311
Feature type
allele
Associated gene
Dmel\Ced-12
Associated Insertion(s)
Carried in Construct
P{UAS-Ced-12.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Ishimaru et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ishimaru et al., 2004)
Mutagen
in vitro construct
(Ishimaru et al., 2004, Ishimaru et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-Ced-12.RNAi}
Cytology
Description

UAS sequences drive expression Ced-12 coding sequences (from GenBank:NM135704 nucleotides 123 to 827) repeated in dyad symmetry.

(Ishimaru et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ced-12
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4slbo.2.6-mediated expression of Ced-12dsRNA.Scer\UAS results in mild defects in border cell migration in which approximately 20% of the border cells fail to migrate completely. Penetrance increases to 40% if two copies of Ced-12dsRNA.Scer\UAS are used.

      Scer\GAL4Mef2.PR-mediated expression of Ced-12dsRNA.Scer\UAS does not result in gross defects in the final muscle pattern. However, an approximately 2-fold increase in unfused myoblasts are observed in stage 16 embryos expressing two copies of Ced-12dsRNA.Scer\UAS compared to controls at 29[o]C.

      (Geisbrecht et al., 2008)

      When mbcdsRNA.Scer\UAS is driven by Scer\GAL4pnr-MD237 mutant adults exhibit defects in thorax closure.

      (Ishimaru et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Ced-12dsRNA.Scer\UAS under the control of Scer\GAL4Act.PU in the cells surrounding scrib1 clones in the eye-antennal disc significantly suppresses the elimination of the mutant scrib1 clones compared to that seen when the cells surrounding the clone are wild type.

      (Ohsawa et al., 2011)

      Co-expression of mbcdsRNA.Scer\UAS and Ced-12dsRNA.Scer\UAS using Scer\GAL4slbo.2.6 increases the severity and penetrance of the border cell migration defects compared to when either transgene is expressed alone, such that 55% of the border cells fail to migrate completely.

      (Geisbrecht et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ced-12RNAi.UAS
      Ced-12dsRNA.Scer\UAS
      Ced-12dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)