FlyBase Allele Report: Dmel\mbc[RNAi.UAS]

General Information

Symbol

Dmel\mbc^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175602

Feature type

allele

Associated gene

Dmel\mbc

Associated Insertion(s)

Carried in Construct

P{UAS-mbc.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Ishimaru et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ishimaru et al., 2004)

Mutagen

in vitro construct

(Ishimaru et al., 2004, Ishimaru et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-mbc.RNAi}

Cytology

Description

UAS sequences drive expression mbc coding sequences (from GenBank:AF007805 nucleotides 609 to 1311) repeated in dyad symmetry.

(Ishimaru et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mbc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Geisbrecht et al., 2008)

thorax, with Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

Detailed Description

Statement

Reference

Scer\GAL4^slbo.2.6-mediated expression of mbc^{dsRNA.Scer\UAS} results in severe defects in border cell migration in which approximately 40% of the stage 10 egg chambers contain cell clusters that fail to reach the nurse cell/oocyte border.

(Geisbrecht et al., 2008)

When mbc^{dsRNA.Scer\UAS} is driven by Scer\GAL4^pnr-MD237 mutant adults exhibit defects in thorax closure.

(Ishimaru et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^slbo.2.6, mbc^RNAi.UAS has border follicle cell phenotype, enhanceable by Ced-12^RNAi.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht et al., 2008)

Scer\GAL4^slbo.2.6, mbc^RNAi.UAS has border follicle cell phenotype, enhanceable by Crk^RNAi.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht et al., 2008)

Enhancer of

Statement

Reference

mbc^RNAi.UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Ced-12^RNAi.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht et al., 2008)

mbc^RNAi.UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Crk^RNAi.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mbc^{dsRNA.Scer\UAS} and Ced-12^{dsRNA.Scer\UAS} using Scer\GAL4^slbo.2.6 increases the severity and penetrance of the border cell migration defects compared to when either transgene is expressed alone, such that 55% of the border cells fail to migrate completely.

Co-expression of mbc^{dsRNA.Scer\UAS} and Crk^{dsRNA.Scer\UAS} using Scer\GAL4^slbo.2.6 increases the severity and penetrance of the border cell migration defects compared to when either transgene is expressed alone, but only slightly.

(Geisbrecht et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mbc^RNAi.UAS

mbc^{dsRNA.Scer\UAS}

mbc^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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