FlyBase Allele Report: Dmel\ssh[1-63]

General Information

Symbol

Dmel\ssh^1-63

Species

D. melanogaster

Name

FlyBase ID

FBal0177648

Feature type

allele

Associated gene

Dmel\ssh

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Ng, 2005.7)

Mutagen

P-element activity

(Ng, 2005.7)

Progenitor genotype

P{PZ}ssh⁰¹²⁰⁷

(Ng, 2005.7)

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Abe et al., 2014)

abnormal neuroanatomy | somatic clone

(Ng and Luo, 2004)

Phenotype Manifest In

adult mushroom body & axon | somatic clone

(Ng and Luo, 2004)

adult mushroom body alpha-lobe

(Abe et al., 2014)

adult mushroom body beta-lobe

(Abe et al., 2014)

antennal lobe & axon | somatic clone

(Ng and Luo, 2004)

mushroom body medial lobe | somatic clone

(Ng and Luo, 2004)

optic lobe neuron & axon | somatic clone

(Ng and Luo, 2004)

vertical lobe of adult mushroom body | somatic clone

(Ng and Luo, 2004)

Detailed Description

Statement

Reference

The mushroom bodies of ssh^1-63 mutant flies show axonal growth defects at the lobe region but not the peduncle.

ssh^1-63 mutant clones of the α/β lobe of the mushroom body show slightly increased F-actin levels at and around the core region compared with wild type controls.

(Abe et al., 2014)

Homozygous mushroom body neuroblast clones in the adult that are generated in newly hatched larvae do not have any obvious reduction in cell numbers compared to control clones. However, these clones show axon growth or axon growth plus guidance defects. In "severe" cases, the axons fail to extend beyond the peduncle and guidance defects are also detected at the peduncle or from the dendrite region, in "strong" cases, axon growth defects are seen in the dorsal and medial branches, and in "weak" cases axon growth defects are seen in the dorsal branch. Homozygous neuroblast clones in the optic lobe or antennal lobe contralateral projection neurons fail to cross the midline, in contrast to wild type.

(Ng and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ssh^1-63 is an enhancer of abnormal neuroanatomy | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Suppressor of

Statement

Reference

ssh^1-63/ssh[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ssh^1-63/ssh[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Other

Statement

Reference

ssh^1-63/ssh[+], tsr¹/tsr^ntf has lethal phenotype

(Kim et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

ssh^1-63 is an enhancer of adult mushroom body alpha-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^1-63 is an enhancer of adult mushroom body beta-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Suppressor of

Statement

Reference

ssh^1-63/ssh[+] is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ssh^1-63/ssh[+] is a suppressor of eye phenotype of mbt^P3

(Menzel et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The axon growth and increased F-actin phenotypes seen in α/β lobe mushroom body clones expressing LIMK1^{Scer\UAS.T:Ivir\HA1} are suppressed by sick^{Scer\UAS.T:Disc\RFP-mCherry}. ssh^1-63 reverses this suppression, with LIMK1^{Scer\UAS.T:Ivir\HA1} sick^{Scer\UAS.T:Disc\RFP-mCherry} ssh^1-63 triple mutant clones showing the more severe highly elevated F-actin and 'posterior arrest' (failure of axons to extend to form peduncles and lobe structures) phenotypes seen in LIMK1^{Scer\UAS.T:Ivir\HA1}, ssh^1-63 mutants. The phenotype seen in LIMK1^{Scer\UAS.T:Ivir\HA1}, ssh^1-63 double mutant clones is more severe than in either single mutant.

(Abe et al., 2014)

Expression of psidin^Scer\UAS.cKa under the control of Scer\GAL4^slbo.2.6 in a ssh^1-63 heterozygous background has little or no effect on border cell migration.

(Kim et al., 2011)

The rough eye phenotype of mbt^P3 hemizygous males is suppressed by ssh^1-63/+.

(Menzel et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9110

y¹ w^*; P{neoFRT}82B ssh^1-63/TM3, y⁺ Ser¹

Notes on Origin

Discoverer

Comments

Null allele. The screen generating this allele was described in FBrf0144752.

(Ng, 2005.7)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ssh^1-63

(Hildebrand et al., 2021, Abe et al., 2014, Kim et al., 2011, Menzel et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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