FlyBase Allele Report: Dmel\tsr[ntf]

General Information

Symbol

Dmel\tsr^ntf

Species

D. melanogaster

Name

FlyBase ID

FBal0140903

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

gypsy{}tsr^ntf

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

gypsy{}tsr^ntf

Cytology

Description

Insertion of a 7.4kb gypsy element 250bp downstream of the 5' end of the tsr first intron. The gypsy LTRs are in the opposite direction to that of the tsr transcript. No mutations are found in the tsr coding region, but two C-to-A mutations are found in the second intron, neither of which are in the splicing consensus sites of the intron.

(Chen et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with tsr¹)

(Chen et al., 2001)

female sterile (with tsr²)

(Chen et al., 2001)

male semi-fertile (with tsr²)

(Chen et al., 2001)

male sterile | heat sensitive (with tsr¹)

(Chen et al., 2001)

partially lethal - majority die

(Chen et al., 2001)

partially lethal - majority live (with tsr¹)

(Chen et al., 2001)

some die during pupal stage

(Chen et al., 2001)

sterile

(Chen et al., 2001)

viable (with tsr²)

(Chen et al., 2001)

Phenotype Manifest In

actin filament | heat sensitive

(Chen et al., 2001)

border follicle cell | heat sensitive (with tsr¹)

(Chen et al., 2001)

chaeta

(Chen et al., 2001)

nurse cell & nucleus (with tsr¹)

(Chen et al., 2001)

ovary | heat sensitive

(Chen et al., 2001)

terminal filament | heat sensitive

(Chen et al., 2001)

Detailed Description

Statement

Reference

tsr¹/tsr^ntf animals are viable at 18[o]C.

(Kim et al., 2011)

Terminal filaments do not form in the larval ovary of homozygotes at 25^oC, but are formed at 18^oC. At 21^oC, terminal filaments form but are not as organised and do not contain as many cells as those in wild-type ovaries. At 25^oC, the terminal filaments and apical cells of the ovary are larger and rounder than normal and the anterior region of the ovary appears elongated. There is a large increase in the amount of filamentous actin in apical and terminal filament cells of the ovary of homozygous females raised at 25^oC (and the filamentous actin is abnormally distributed throughout the cytoplasm) and a slight increase in the level of filamentous actin in the germline in these females compared to wild type. A few homozygous adult escapers are seen, they are male sterile and have a gnarled bristle phenotype. tsr¹/tsr^ntf animals are semi-viable at 25^oC; only males survive and are sterile. At 18^oC, these animals are viable and some males are fertile, while females are sterile and show defects in border cell migration. The border cell migration defects are more severe at higher temperatures. Some egg chambers show "dumping" of nurse cell nuclei into the oocyte at 25^oC. Terminal filaments are present in the larval ovary of tsr¹/tsr^ntf animals. Terminal filaments are present in the larval ovary of tsr²/tsr^ntf animals.

(Chen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

psidin^55D4/psidin[+], tsr¹/tsr^ntf has lethal phenotype

(Kim et al., 2011)

psidin^85D1/psidin[+], tsr¹/tsr^ntf has lethal phenotype

(Kim et al., 2011)

psidin[+]/psidin^e02846, tsr¹/tsr^ntf has lethal phenotype

(Kim et al., 2011)

ssh^1-63/ssh[+], tsr¹/tsr^ntf has lethal phenotype

(Kim et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

tsr^ntf/tsr² is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Sese et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

tsr²/tsr^ntf weakly suppresses the extra R7 photoreceptor cell phenotype of sev^{S11.T:Hsap\MYC} flies.

(Sese et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tsr^ntf is rescued by tsr^+t6.4

(Chen et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The tsr^ntf mutation was found in the l(2)k08106 line which contains five independent P-element insertions, although none of the insertions causes the tsr^ntf mutation.

(Chen et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ntf

(Hudson and Cooley, 2002, Chen et al., 2001)

tsr^ntf

(Wu et al., 2016, Kim et al., 2011, Sese et al., 2006)

Name Synonyms

no terminal filament

(Hudson and Cooley, 2002)

no terminal filaments

(Chen et al., 2001)

Secondary FlyBase IDs

References (5)

Report Sections

Open Close