FlyBase Allele Report: Dmel\spas[T32]

General Information

Symbol

Dmel\spas^T32

Species

D. melanogaster

Name

FlyBase ID

FBal0177734

Feature type

allele

Associated gene

Dmel\spas

Associated Insertion(s)

P{EP}spas^T32

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sherwood et al., 2004)

Progenitor genotype

Associated Insertion(s)

P{EP}spas^T32

Cytology

Description

P{EP} insertion is upstream of the coding start of the spas gene, being 58 nucleotides after the transcriptional start site.

(Sherwood et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary spastic paraplegia 4

CEC

(Ozdowski et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of hereditary spastic paraplegia 4

is ameliorated by Pak3^Df

(Ozdowski et al., 2011)

is exacerbated by Pak3^LA00012

(Ozdowski et al., 2011)

is ameliorated by Pak3^d02472

(Ozdowski et al., 2011)

is ameliorated by Pak3^e00329

(Ozdowski et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone, with Scer\GAL4^ppk.PG

(Ye et al., 2011)

visible, with Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

Phenotype Manifest In

dendritic arborizing neuron | somatic clone, with Scer\GAL4^ppk.PG

(Ye et al., 2011)

eye, with Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

larval ventral nerve cord, with Scer\GAL4^sca-537.4

(Sherwood et al., 2004)

Detailed Description

Statement

Reference

Expression of spas^T32 under the control of Scer\GAL4^unspecified in either ddaC or ddaE neurons does not effect the multipolar morphological type of dendritic arborizing neurons.

(Wang et al., 2015)

Expression of spas^T32 under the control of Scer\GAL4^GMR.PU results in eye size reduction and smoother texture.

(Ozdowski et al., 2011)

spas^T32 mutant overexpression (through Scer\GAL4^ppk.PG) leads to a dramatic reduction in both total dendritic length and branch number. Similarly, the number of branch points are reduced. spas upregulation by expression of spas^T32 results in a mild yet significant reduction in axonal growth in class IV da neurons.

(Ye et al., 2011)

Overexpression of spas^T32 in the hindgut epithelium (under the control of Scer\GAL4^NP2432) does not affect the laterality of this organ.

(Taniguchi et al., 2007)

When spas^T32 is driven by Scer\GAL4^sca-537.4, at 23^oC, mutant embryos embryos display abnormal midline crossing of the inner 1D4 bundle, and the entire ventral nerve cord (VNC) is narrowed at these crossing sites. At 29^oC the VNC collapses onto the midline and discrete longitudinal bundles are no longer apparent.

(Sherwood et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, enhanceable by Pak3^LA00012, Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, suppressible | partially by Df(3R)Exel6269

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, suppressible | partially by Pak3^Df

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, suppressible | partially by Pak3^d02472

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, suppressible | partially by Pak3^e00329

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has visible phenotype, suppressible | partially by Pak3^GD8481, Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, enhanceable by Pak3^LA00012, Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, suppressible | partially by Df(3R)Exel6269

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, suppressible | partially by Pak3^Df

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, suppressible | partially by Pak3^d02472

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, suppressible | partially by Pak3^e00329

(Ozdowski et al., 2011)

Scer\GAL4^GMR.PU, spas^T32 has eye phenotype, suppressible | partially by Pak3^GD8481, Scer\GAL4^GMR.PU

(Ozdowski et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Df(3R)Exel6269 partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

Pak3^Df partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

Pak3^d02472 partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

Pak3^e00329 partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Pak3^GD8481 partially suppresses the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Pak3^LA00012 enhances the eye phenotype seen when spas^T32 is expressed under the control of Scer\GAL4^GMR.PU.

(Ozdowski et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

spas^T32

(Ozdowski et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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