Insertion site is 345 bp upstream of the first exon of the ru cDNA LP02893. Insertion originated in the collection of insertion lines described in FBrf0099762.
In stage 16 ruinga mutant and ruinga/Df(3L)Ar14-8 embryos, a significant number of ganglionic branch trachea fail to turn posteriorly and dorsally at the ventral midline and instead cross the midline or remain lingering on it.