FlyBase Allele Report: Dmel\mir-7[UAS.DsRed2]

General Information

Symbol

Dmel\mir-7^UAS.DsRed2

Species

D. melanogaster

Name

FlyBase ID

FBal0178864

Feature type

allele

Associated gene

Dmel\mir-7

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed2-mir-7}

Also Known As

UAS-DsRed-miR-7, UAS-miR-7

Key Links

Transgenic product class

wild_type

(Stark et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Stark et al., 2003)

Mutagen

in vitro construct

(Brennecke et al., 2005, Stark et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-DsRed2-mir-7}

Cytology

Description

UASt regulatory sequences drive expression of DsRed2. A genomic fragment containing the mir-7 hairpin has been cloned into the 3' UTR sequences.

(Stark et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mir-7

Tagged with

DsRed2

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^da.PU

(Bejarano et al., 2012)

some die during larval stage, with Scer\GAL4^da.PU

(Bejarano et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012, Bejarano et al., 2012)

visible, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Bejarano et al., 2012, Bejarano et al., 2012, Stark et al., 2003)

visible, with Scer\GAL4^sca.PU

(Szuplewski et al., 2012)

visible, with Scer\GAL4^sd.PU

(Aparicio et al., 2015)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012, Bejarano et al., 2012)

Phenotype Manifest In

chaeta | ectopic, with Scer\GAL4^sca.PU

(Szuplewski et al., 2012)

first posterior wing cell, with Scer\GAL4^ptc-559.1

(Bejarano et al., 2012, Stark et al., 2003)

scutellar bristle | ectopic, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

sternopleural bristle | ectopic, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

wing, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

wing, with Scer\GAL4^ptc-559.1

(Bejarano et al., 2012, Bejarano et al., 2012, Stark et al., 2003)

wing, with Scer\GAL4^sd.PU

(Aparicio et al., 2015)

wing, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012, Bejarano et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012, Bejarano et al., 2012)

wing vein, with Scer\GAL4^sd.PU

(Aparicio et al., 2015)

wing vein, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} driven by Scer\GAL4^sd.PU leads to wing phenotypes: notches, thickened veins and a decrease in the L3-L4 intervein domain phenotype.

(Aparicio et al., 2015)

Expression of mir-10^Scer\UAS.cGa under the control of Scer\GAL4^Mhc.PW has no significant effect on adult lifespan relative to controls.

(Fernandez-Costa et al., 2013)

Animals expressing mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^da.G32 die as first or second instar larvae.

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^Bx-MS1096 results in curved and often folded wings. Wing veins are thicker than normal, especially veins L3 and L5.

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^sd-SG29.1 results in strong wing notching and thicker veins.

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^ptc-559.1 results in wing notching.

(Bejarano et al., 2012)

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^Bx-MS1096 results in massive thickening of the wing veins, but the margin is continuous.

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^sd-SG29.1 results in massive notching of the wing, with mild thickening of the wing veins.

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^ptc-559.1 results in distal wing notching and reduction in the L3-L4 intervein domain.

(Bejarano et al., 2012)

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^sca.PU results in extra bristles.

(Szuplewski et al., 2012)

Flies expressing mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^dpp.blk1 often have ectopic sternopleural and scutellar bristles and show wing notching.

(Li et al., 2009)

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^ptc-559.1 results in notching of the wing and a reduction of the region between wing veins 3 and 4, leading to partial fusion proximally.

(Stark et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/mir-7^UAS.DsRed2 is a non-suppressor of visible phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/mir-7^UAS.DsRed2 is a suppressor of eye phenotype of Df(2R)exu1/Df(2R)mir-7-Δ1, aop^ACT.GMR

(Li and Carthew, 2005)

Scer\GAL4^GMR.PF/mir-7^UAS.DsRed2 is a suppressor of photoreceptor neuron phenotype of Df(2R)exu1/Df(2R)mir-7-Δ1, aop^ACT.GMR

(Li and Carthew, 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/mir-7^UAS.DsRed2 is a non-suppressor of macrochaeta phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced eye phenotype seen in aop^ACT.GMR flies is enhanced in aop^ACT.GMR Df(2R)mir-7-Δ1/Df(2R)exu1 flies, such that these double mutants have, on average, just 3.7 photoreceptors per ommatidium. Coexpression of the mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} transgene, under the control of Scer\GAL4^GMR.PF, prevents the enhancement caused by Df(2R)mir-7-Δ1/Df(2R)exu1.

(Li and Carthew, 2005)

Xenogenetic Interactions

Statement

Reference

The reduced median lifespan of adult flies expressing Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^Mhc.PW is significantly worsened by co-expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2}, though the effect is mild and attributable to the toxicity of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} expression even on its own.

(Fernandez-Costa et al., 2013)

Complementation and Rescue Data

Partially rescues

Scer\GAL4^sd.PU/mir-7^UAS.DsRed2 partially rescues Df(2R)exu1/Df(2R)mir-7-Δ1

(Aparicio et al., 2015)

Comments

Expression of mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} driven by Scer\GAL4^sd.PU partially suppresses wing phenotypes seen in Df(2R)mir-7-Δ1/Df(2R)exu1 flies.

(Aparicio et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (3)