FlyBase Allele Report: Dmel\ctp[DIIA82]

General Information

Symbol

Dmel\ctp^DIIA82

Species

D. melanogaster

Name

FlyBase ID

FBal0180294

Feature type

allele

Associated gene

Dmel\ctp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Ghosh-Roy et al., 2005, Ghosh-Roy et al., 2004)

Mutagen

Delta2-3 transposase

(Ghosh-Roy et al., 2004)

Progenitor genotype

ctp^ins1

(Ghosh-Roy et al., 2004)

Cytology

Description

P{lacW} reinsertion line.

(Ghosh-Roy et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Ghosh-Roy et al., 2004)

lethal | heat sensitive (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

male semi-sterile

(Ghosh-Roy et al., 2004)

partially lethal - majority live

(Ghosh-Roy et al., 2004)

visible (with ctp^DIA5)

(Ghosh-Roy et al., 2004)

visible (with ctp^DIIB14)

(Ghosh-Roy et al., 2004)

visible (with ctp^exc6)

(Ghosh-Roy et al., 2004)

visible (with ctp^exc41)

(Ghosh-Roy et al., 2004)

visible (with ctp^ins1)

(Ghosh-Roy et al., 2004)

visible (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

visible | recessive

(Ghosh-Roy et al., 2004)

Phenotype Manifest In

actin filament & spermatid

(Ghosh-Roy et al., 2005)

actin filament bundle & nucleus

(Ghosh-Roy et al., 2005)

cystic bulge

(Ghosh-Roy et al., 2005)

macrochaeta

(Ghosh-Roy et al., 2004)

macrochaeta (with ctp^DIA5)

(Ghosh-Roy et al., 2004)

macrochaeta (with ctp^DIIB14)

(Ghosh-Roy et al., 2004)

macrochaeta (with ctp^exc6)

(Ghosh-Roy et al., 2004)

macrochaeta (with ctp^exc41)

(Ghosh-Roy et al., 2004)

macrochaeta (with ctp^ins1)

(Ghosh-Roy et al., 2004)

macrochaeta (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

seminal vesicle

(Ghosh-Roy et al., 2004)

spermatid

(Ghosh-Roy et al., 2004)

spermatid cyst

(Ghosh-Roy et al., 2004)

spermatocyte fusome

(Ghosh-Roy et al., 2004)

spermatozoon

(Ghosh-Roy et al., 2004)

wing

(Ghosh-Roy et al., 2004)

wing (with ctp^DIA5)

(Ghosh-Roy et al., 2004)

wing (with ctp^DIIB14)

(Ghosh-Roy et al., 2004)

wing (with ctp^exc6)

(Ghosh-Roy et al., 2004)

wing (with ctp^exc41)

(Ghosh-Roy et al., 2004)

wing (with ctp^ins1)

(Ghosh-Roy et al., 2004)

wing (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

Detailed Description

Statement

Reference

The number of nuclear bundles and individualization complexes is significantly decreased in the testis of ctp^DIIA82 mutants compared to controls. The F-actin is decreased in the investment cones during ctp^DIIA82 sperm individualization compared to wild-type controls. This phenotype is not temperature sensitive.

(Ghosh-Roy et al., 2005)

ctp^DIIA82 homozygotes and the following transheterozygotes exhibit thin bristles and erect wings: ctp^DIIA82/ctp^DIIB14, ctp^DIIA82/ctp^exc41, ctp^DIIA82/ctp^DIA5, ctp^DIIA82/ctp^ins1, ctp^exc6/ctp^DIIA82 and ctp^DIIA82/Df(1)bi-DL1. The seminal vesicles of ctp^DIIA82 hemizygous males contain drastically lower numbers of motile sperm than wild type. Any motile sperm are also more sluggish than in wild type. The testes of 2 day-old ctp^DIIA82 mutants contain fewer cysts than wild type; the mutant cysts appear to be shorter and contain contain spermatids that are abnormally bundled in multiple fascicles within a single cyst. There are very few elongation cones (ECs) in the testis of ctp^DIIA82 males and those present are deformed. The average length of cysts is shortened in ctp^ins1 testes, as the peak of the elongation cone (EC) distribution profile is shifted to the basal end. The ctp^DIIA82 mutation affects the cellularization of spermatids. Mature cysts show incompletely individualized spermatids with multiple axoneme-mitochondria sets within one cellular envelope that are interconnected by thin membranous bridges. The organization of axonemal microtubules appears to be normal.

(Ghosh-Roy et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ctp^DIIA82 has male semi-sterile phenotype, suppressible by Dhc64C[+]/Dhc64C^4-19

(Ghosh-Roy et al., 2004)

Other

Statement

Reference

ctp^DIIA82, sw¹ has lethal phenotype

(Ghosh-Roy et al., 2005)

DCTN1-p150^Gl-1, ctp^DIIA82 has lethal phenotype

(Ghosh-Roy et al., 2004)

Gl[+]/DCTN1-p150^Gl-1, ctp^DIIA82 has lethal phenotype

(Ghosh-Roy et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

ctp^DIIA82 is an enhancer of actin filament & spermatid | conditional ts phenotype of shi¹

(Ghosh-Roy et al., 2005)

ctp^DIIA82 is an enhancer of actin filament & spermatid | conditional ts phenotype of shi²

(Ghosh-Roy et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Almost all ctp^ins1; sw¹/Y males fail to emerge, at either 25^oC or 18^oC.

The F-actin density in the investment cones of spermatid individualization complexes is further decreased following a heat pulse of 11 hours in ctp^DIIA82, shi² double mutants compared to shi² single mutants. The same phenotype is enhanced in ctp^DIIA82, shi¹ double mutants compared to shi¹ single mutants.

(Ghosh-Roy et al., 2005)

The ctp^DIIA82 mutation is lethal in combination with one copy of Gl¹. The sterility of ctp^DIIA82 males is partially suppressed when they carry one copy of Dhc64C^4-19.

(Ghosh-Roy et al., 2004)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\DNCL1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the disruption of nuclear bundles and individualization complexes in ctp^DIIA82 mutant testis.

(Ghosh-Roy et al., 2005)

Expression of Hsap\DNCL1^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, rescues the reduced fertility phenotype of ctp^DIIA82/Y males. This rescue includes increasing the amount of motile sperm within the seminal vesicles and increasing the amount and length of ECs.

(Ghosh-Roy et al., 2004)

Complementation and Rescue Data

Rescued by

ctp^DIIA82 is rescued by Scer\GAL4^{VP16.nanos.UTR}/ctp^UASp.cGa

(Ghosh-Roy et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series in order of increasing strength based on testis phenotype: ctp^exc39 < ctp^ins1 < ctp^DIIA82.

(Ghosh-Roy et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DIIA82

(Ghosh-Roy et al., 2005)

LC8^DIIA82

(Parassol et al., 2013)

ctp^DIIA82

ddlc1^DIIA82

(Ghosh-Roy et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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