FlyBase Allele Report: Dmel\ctp[ins1]

The number of nuclear bundles and individualization complexes is significantly decreased in the testis of ctp^ins1 mutants compared to controls. The F-actin is decreased in the investment cones during ctp^ins1 sperm individualization compared to wild-type controls. This phenotype is not temperature sensitive.

(Ghosh-Roy et al., 2005)

ctp^ins1 flies have thin bristles, abnormal wing veins and erect wings. The following transheterozygotes also exhibit thin bristles and erect wings: ctp^ins1/ctp^DIIB14, ctp^ins1/ctp^exc41, ctp^ins1/ctp^DIA5, and ctp^DIIA82/ctp^ins1. ctp^ins1 males are sterile, and microscopic analysis of their testes shows that spermatids are abnormally coiled in the cysts. The seminal vesicles of ctp^ins1 hemizygous males contain drastically lower numbers of motile sperm than wild type. Any motile sperm are also more sluggish than in wild type. The total number of elongation cones (ECs) per testis is reduced in ctp^ins1 mutants and a large proportion of these ECs are deformed. The average length of cysts are shortened in ctp^ins1 testes, as the peak of the elongation cone (EC) distribution profile is shifted to the basal end.

(Ghosh-Roy et al., 2004)

Homozygous female wings have a small delta at the end of the wing veins and the bristles are thinner than wild type. Ovarioles and egg chambers are disorganised (defects are variable), disorganisation is more severe when transheterozygous with ctp^exc3 or ctp^exc6. Transheterozygotes with Df(1)ovo6 exhibit ovarian disorganisation phenotype.

(Dick et al., 1996)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ctp^ins1 has male semi-sterile phenotype, suppressible by Dhc64C[+]/Dhc64C^4-19

(Ghosh-Roy et al., 2004)

Other

Statement

Reference

ctp^ins1, sw¹ has lethal phenotype

(Ghosh-Roy et al., 2005)

DCTN1-p150^Gl-1, ctp^ins1 has male sterile phenotype

(Ghosh-Roy et al., 2004)

Df(3L)fz-GF3b/+, ctp^ins1 has male sterile phenotype

(Ghosh-Roy et al., 2004)

Gl[+]/DCTN1-p150^Gl-1, ctp^ins1 has male sterile phenotype

(Ghosh-Roy et al., 2004)

Df(3L)GN24/+, ctp^ins1 has male sterile phenotype

(Ghosh-Roy et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

ctp^ins1 has cystic bulge phenotype, enhanceable by Gl[+]/DCTN1-p150^Gl-1

(Ghosh-Roy et al., 2005)

ctp^ins1 has nucleus & spermatid phenotype, enhanceable by Gl[+]/DCTN1-p150^Gl-1

(Ghosh-Roy et al., 2005)

ctp^ins1 has spermatid cyst phenotype, enhanceable by Df(3L)fz-GF3b/+

(Ghosh-Roy et al., 2004)

ctp^ins1 has spermatocyte fusome phenotype, enhanceable by Df(3L)fz-GF3b/+

(Ghosh-Roy et al., 2004)

ctp^ins1 has spermatid cyst phenotype, enhanceable by Gl[+]/DCTN1-p150^Gl-1

(Ghosh-Roy et al., 2004)

ctp^ins1 has spermatocyte fusome phenotype, enhanceable by Gl[+]/DCTN1-p150^Gl-1

(Ghosh-Roy et al., 2004)

NOT Enhanced by

Statement

Reference

ctp^ins1 has cystic bulge phenotype, non-enhanceable by Dhc64C^4-19

(Ghosh-Roy et al., 2005)

ctp^ins1 has nucleus & spermatid phenotype, non-enhanceable by Dhc64C^4-19

(Ghosh-Roy et al., 2005)

Suppressed by

Statement

Reference

ctp^ins1 has spermatocyte fusome phenotype, suppressible by Dhc64C[+]/Dhc64C^4-19

(Ghosh-Roy et al., 2004)

Enhancer of

Statement

Reference

ctp^ins1 is an enhancer of actin filament & spermatid | conditional ts phenotype of shi¹

(Ghosh-Roy et al., 2005)

ctp^ins1 is an enhancer of actin filament & spermatid | conditional ts phenotype of shi²

(Ghosh-Roy et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Almost all ctp^ins1; sw¹/Y males fail to emerge, at either 25^oC or 18^oC.

The ctp^ins1/Y nuclear bundles and individualization complex phenotype is dominantly enhanced by Gl¹. In contrast, the Dhc64C^4-19 allele does not affect this phenotype.

The F-actin density in the investment cones of spermatid individualization complexes is further decreased following a heat pulse of 11 hours in ctp^ins1, shi² double mutants compared to shi² single mutants. The same phenotype is enhanced in ctp^ins1, shi¹ double mutants compared to shi¹ single mutants.

(Ghosh-Roy et al., 2005)

The sterility of ctp^ins1 males is enhanced to 100% when they are heterozygous for either Gl¹ or Df(3L)fz-GF3b. In contrast, the sterility of ctp^ins1 mutants is suppressed when they carry one copy of Dhc64C^4-19, although sterility is enhanced to 100% in ctp^ins1; Df(3L)GN24/+ males. In ctp^ins1; Gl¹ males, and ctp^ins1; Df(3L)fz-GF3b males there is a higher proportion of deformed ECs and cyst length is further shortened compared to ctp^ins1 single mutants. The Dhc64C^4-19 mutation suppresses the testicular phenotype of ctp^ins1 males; these double mutants have greater numbers of ECs within each cyst and a higher proportion of these are intact. However, the EC distribution profile is not altered.

(Ghosh-Roy et al., 2004)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\DNCL1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the disruption of nuclear bundles and individualization complexes in ctp^ins1 mutant testis.

(Ghosh-Roy et al., 2005)

Expression of Hsap\DNCL1^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, rescues the reduced fertility phenotype of ctp^ins1/Y males. This rescue includes increasing the amount of motile sperm within the seminal vesicles and increasing the amount and length of ECs.

(Ghosh-Roy et al., 2004)

Complementation and Rescue Data

Rescued by

ctp^ins1 is rescued by Scer\GAL4^{VP16.nanos.UTR}/ctp^UASp.cGa

(Ghosh-Roy et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{lacW} element reverts the female adult defects confirming these abnormalities are caused by the P{lacW} element.

(Dick et al., 1996)

Allelic series in order of increasing strength based on testis phenotype: ctp^exc39 < ctp^ins1 < ctp^DIIA82.

(Ghosh-Roy et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cdlc1^ins1

LC8^ins1

(Parassol et al., 2013)

ctp^ins1

ddlc1^ins1

(Ghosh-Roy et al., 2004, Dick et al., 1997, Dick et al., 1996)

ins1

(Ghosh-Roy et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (5)

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