FlyBase Allele Report: Dmel\Roc1b[F28M]

General Information

Symbol

Dmel\Roc1b^F28M

Species

D. melanogaster

Name

FlyBase ID

FBal0181844

Feature type

allele

Associated gene

Dmel\Roc1b

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Donaldson et al., 2004)

Mutagen

I-SceI

(Donaldson et al., 2004)

I-CreI

(Donaldson et al., 2004)

recombination

(Donaldson et al., 2004)

FLPase

(Donaldson et al., 2004)

gene targeting by homologous recombination

(Donaldson et al., 2004)

Progenitor genotype

Cytology

Description

A frameshift/premature stop codon has been introduced after the fifth codon of the Roc1b open reading frame.

(Donaldson et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-sterile

(Donaldson et al., 2004)

male sterile (with Df(3L)emc-E12)

(Donaldson et al., 2004)

male sterile (with Roc1b^dc3)

(Donaldson et al., 2004)

viable

(Donaldson et al., 2004)

Phenotype Manifest In

spermatozoon

(Donaldson et al., 2004)

Detailed Description

Statement

Reference

Around 2% of eggs hatch when wild-type females are mated with Roc1b^F28M males. Roc1b^F28M males do produce live, motile sperm but in quantities that are much lower than wild type.

(Donaldson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Roc1b^F28M has male semi-sterile phenotype, suppressible by Roc1a^grf.Roc1b

(Donaldson et al., 2004)

NOT suppressed by

Statement

Reference

Roc1b^F28M has male semi-sterile phenotype, non-suppressible by Roc2^grf.Roc1b

(Donaldson et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The male sterility of Roc1b^F28M flies is partially rescued by expression of the transgene Roc1a^grf.Roc1b.

(Donaldson et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Roc1b^F28M is partially rescued by Roc1b^+t1.17

(Donaldson et al., 2004)

Comments

Expression of Roc1b^+t1.17 rescues the fertility of Roc1b^F28M males to ~70% of wild-type levels.

(Donaldson et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Roc1b^F28M

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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