FlyBase Allele Report: Dmel\Nedd4[GS13178]

General Information

Symbol

Dmel\Nedd4^GS13178

Species

D. melanogaster

Name

FlyBase ID

FBal0182548

Feature type

allele

Associated gene

Dmel\Nedd4

Associated Insertion(s)

P{GSV6}GS13178

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV6}GS13178

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GSV6}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:17,529,817..17,529,817

(Dalton et al., 2011, Jaekel and Klein, 2006, Sakata, 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Sakata, 2004)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Sakata, 2004)

partially lethal - majority die, with Scer\GAL4^ptc-559.1

(Dalton et al., 2011)

some die during larval stage, with Scer\GAL4^da.G32

(Sakata, 2004)

viable

(Sakata, 2004)

visible, with Scer\GAL4^Dll-md23

(Sakata, 2004)

visible, with Scer\GAL4^ptc-559.1

(Sakata, 2004, Sakata et al., 2004)

Phenotype Manifest In

wing, with Scer\GAL4^Dll-md23

(Sakata, 2004)

wing, with Scer\GAL4^ptc-559.1

(Sakata et al., 2004)

wing margin, with Scer\GAL4^ptc-559.1

(Dalton et al., 2011, Sakata, 2004)

wing margin bristle, with Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

wing vein, with Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

wing vein, with Scer\GAL4^Dll-md23

(Sakata, 2004)

wing vein, with Scer\GAL4^ptc-559.1

(Dalton et al., 2011)

wing vein L3, with Scer\GAL4^ptc-559.1

(Sakata, 2004)

Detailed Description

Statement

Reference

Overexpression of Nedd4^GS13178 using Scer\GAL4^ptc-559.1 results in a disrupted wing with thick veins and loss of the adult wing margin.

Expression of Nedd4^GS13178 using Scer\GAL4^ptc-559.1 results in significant mortality.

Overexpression of Nedd4^GS13178 using Scer\GAL4^Bx-MS1096 results in a severely disrupted wing with thick veins and a loss of bristles at the wing margin.

(Dalton et al., 2011)

Expression of Nedd4^GS13178 under the control of Scer\GAL4^ptc-559.1 results in a Notch-like phenotype in the wing.

(Sakata et al., 2004)

Expression of Nedd4^GS13178 under the control of Scer\GAL4^ptc-559.1 results in a gap in the wing margin and thickening of the L3 wing vein. The wing phenotype caused by expression of Nedd4^GS13178 under the control of Scer\GAL4^ptc-559.1 is suppressed by Nedd4^C974FS.

(Sakata, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Nedd4^GS13178, Scer\GAL4^ptc-559.1 has wing margin phenotype, enhanceable by Ndfip^UAS.Tag:HA, Scer\GAL4^ptc-559.1

(Dalton et al., 2011)

Nedd4^GS13178, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by Ndfip^UAS.Tag:HA, Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

Other

Statement

Reference

Ndfip^UAS.Tag:HA, Nedd4^GS13178, Scer\GAL4^Bx-MS1096 has wing disc phenotype

(Dalton et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Nedd4^GS13178, Scer\GAL4^ptc-559.1 wing phenotype, with severe loss of the wing margin producing smaller wings.

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Nedd4^GS13178, Scer\GAL4^Bx-MS1096 wing phenotype, with little intervein space evident, thick veins and smaller wings.

Wings discs that co-express Nedd4^GS13178 and Ndfip^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 are quite undulated and wrinkled, unlike wild type.

(Dalton et al., 2011)

The wing phenotype seen in flies co-expressing Nedd4^GS13178 and dx^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1 resembles that seen in flies expressing Nedd4^GS13178 alone under the control of Scer\GAL4^ptc-559.1. The wing phenotype seen in flies co-expressing Nedd4^GS13178 and N^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1 resembles that seen in flies expressing Nedd4^GS13178 alone under the control of Scer\GAL4^ptc-559.1.