FlyBase Allele Report: Dmel\Lam[K2]

General Information

Symbol

Dmel\Lam^K2

Species

D. melanogaster

Name

FlyBase ID

FBal0182964

Feature type

allele

Associated gene

Dmel\Lam

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Patterson et al., 2004)

Mutagen

X ray

(Patterson et al., 2004)

Progenitor genotype

Cytology

Description

Addition of the nucleotides CTGC between G460 and A461 results in a frameshift after amino acid 153.

(Patterson et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion

2L:5,543,791..5,543,792 [+]

Comment:

Addition of the nucleotides CTGC between G460 and A461 results in a frameshift after amino acid 153.

Inserted_sequence:

CTGC

(Patterson et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia (with Lam^D395)

(Chen et al., 2014)

partially lethal - majority live (with Df(2L)cl-h4)

(Patterson et al., 2004)

partially lethal - majority live | recessive

(Patterson et al., 2004)

Phenotype Manifest In

adult brain

(Napoletano et al., 2021)

adult midgut (with Lam^D395)

(Chen et al., 2014)

embryonic/larval hypodermal muscle cell | larval stage

(Yamamoto-Hino et al., 2024)

eye

(Patterson et al., 2004)

nuclear membrane | adult stage

(Napoletano et al., 2021)

nucleus | larval stage

(Yamamoto-Hino et al., 2024)

Detailed Description

Statement

Reference

In Lam^K2 heterozygotes, the adult brain exhibits nuclear envelope invaginations.

(Napoletano et al., 2021)

Five day-old Lam^D395/Lam^K2 mutant flies exhibit severe midgut hyperplasia, equivalent to that seen in 50 day-old wild type flies.

Suppressing secretion in fat body cells expressing Scer\GAL4^Cg.PA results in a reduction in gut hyperplasia in Lam^D395/Lam^K2 mutant flies.

(Chen et al., 2014)

Lam^K2 adults have external eye defects.

(Patterson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lam^K2/Lam^D395 has hyperplasia phenotype, suppressible | partially by key^GD1249/Scer\GAL4^Cg.PA

(Chen et al., 2014)

Lam^K2/Lam^D395 has hyperplasia phenotype, suppressible | partially by PGRP-SC2^UAS.cCa/Scer\GAL4^Cg.PA/PGRP-LB^UAS.YFP

(Chen et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lam^K2/Lam^D395 has adult midgut phenotype, suppressible | partially by key^GD1249/Scer\GAL4^Cg.PA

(Chen et al., 2014)

Lam^K2/Lam^D395 has adult midgut phenotype, suppressible | partially by PGRP-SC2^UAS.cCa/Scer\GAL4^Cg.PA/PGRP-LB^UAS.YFP

(Chen et al., 2014)

Enhancer of

Statement

Reference

Lam^K2 is an enhancer of eye phenotype of klar^glrs.Tag:MYC

(Patterson et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of key^GD1249 under the control of Scer\GAL4^Cg.PA suppresses the increased intestinal hyperplasia seen in Lam^D395/Lam^K2 mutant flies.

Co-expression of PGRP-LB^{Scer\UAS.T:Avic\GFP-YFP} and PGRP-SC2^Scer\UAS.cCa under the control of Scer\GAL4^Cg.PA suppresses the increased intestinal hyperplasia seen in Lam^D395/Lam^K2 mutant flies.

(Chen et al., 2014)

Lam^K2 enhances the rough eye phenotype of klar^{glrs.T:Hsap\MYC} mutants.

(Patterson et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lam⁰⁴⁶⁴³

(Patterson et al., 2004)

Lam^P

(Patterson et al., 2004)

Rescued by

Lam^K2 is rescued by Lam^+tLa

(Patterson et al., 2004)

Partially rescued by

Lam^K2/Lam^D395 is partially rescued by Lam^UAS.cMAa

(Chen et al., 2014)

Comments

Expression of Lam^{Scer\UAS.cMAa} in the fat body under the control of Scer\GAL4^Cg.PA significantly rescues the intestinal hyperplasia seen in Lam^D395/Lam^K2 mutant flies. The eclosion rate is increased from ~8% to ~40%.

(Chen et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25093

Lam^K2 pr¹/CyO; st¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lam^K2

(Yamamoto-Hino et al., 2024, Cavaliere et al., 2020)

lamB^K2

(Napoletano et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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