FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\LamA25
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General Information
Symbol
Dmel\LamA25
Species
D. melanogaster
Name
FlyBase ID
FBal0182967
Feature type
allele
Associated gene
Dmel\Lam
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Patterson et al., 2004)
Mutagen
X ray
(Patterson et al., 2004)
Progenitor genotype
Cytology
Description

Deletion of the nucleotides GATCC and the insertion of TCTACCA results in a frameshift after amino acid 611. The deletion removes the C-terminal CaaX box.

(Patterson et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:5,545,470..5,545,474 [+]
Inserted_sequence:

TCTACCA

Comment:

The substitution of TCTACCA for GATCC starting at codon 611 of Lam leads to a frameshift. This leads to early translation termination after the addition of 26 novel amino acids.

(Bondarenko and Sharakhov, 2020, Patterson et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  myopathy
      CEA
      (Frost et al., 2016, Patterson et al., 2004)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      LamA25 adults have some defective ommatidia in the eye. However, the eye discs of LamA25 third instar larvae are indistinguishable from wild-type. LamA25/Df(2L)cl-h4 adults have a more severe eye morphology defect than LamA25 mutants, with misshapen rhabdomeres. Additionally, in the eye discs of LamA25/Df(2L)cl-h4 third instar larvae, there is a defective nuclear migration phenotype in which the nuclei of R-cells are spread throughout the apical/basal axis of the disc, instead of being exclusively apical, as in wild type.

      (Patterson et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      LamA25 is an enhancer of eye phenotype of klarglrs.Tag:MYC

      (Patterson et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      LamA25 enhances the rough eye phenotype of klarglrs.T:Hsap\MYC mutants.

      (Patterson et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Lam04643

      (Patterson et al., 2004)

      LamP

      (Patterson et al., 2004)
      Rescued by

      LamA25 is rescued by Lam+tLa

      (Patterson et al., 2004)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      LamA25
      (Chavan et al., 2024, Ryan et al., 2021, Bondarenko and Sharakhov, 2020, Cornelison et al., 2019, Frost et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)