FlyBase Allele Report: Dmel\Gli[UAS.cSa]

General Information

Symbol

Dmel\Gli^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schulte

FlyBase ID

FBal0183424

Feature type

allele

Associated gene

Dmel\Gli

Associated Insertion(s)

Carried in Construct

P{UAS-Gli.S}

Also Known As

UAS-GliWT

Key Links

Transgenic product class

cDNA

(Schulte et al., 2003)

wild_type

(Schulte et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Schulte et al., 2003)

wild_type

(Schulte et al., 2003)

Mutagen

in vitro construct

(Schulte et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Gli.S}

Cytology

Description

UASt sequences regulate expression of a 3.9kb full-length Gli cDNA.

(Schulte et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gli

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Phenotype Manifest In

wing disc, with Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Detailed Description

Statement

Reference

Expression of Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU in the wing disc results in apoptosis and the migration of delaminated cells (from the expressing side of the disc) into the non-expressing side of the disc.

(Sharifkhodaei et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, enhanceable by tkv^DN.UAS.cUa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, enhanceable by mir-184^{sponge.V2.UAS.mCherry}, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, enhanceable by Dad^EP3196, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Suppressed by

Statement

Reference

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, suppressible by tkv^QD.UAS.cUa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, suppressible by mir-184^UAS.cIa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has increased cell death phenotype, suppressible by Dad^j1E4/Dad[+]

(Sharifkhodaei et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, enhanceable by tkv^DN.UAS.cUa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, enhanceable by mir-184^{sponge.V2.UAS.mCherry}, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, enhanceable by Dad^EP3196, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Suppressed by

Statement

Reference

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, suppressible by tkv^QD.UAS.cUa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, suppressible by mir-184^UAS.cIa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Gli^UAS.cSa, Scer\GAL4^ap.PU has wing disc phenotype, suppressible by Dad^j1E4/Dad[+]

(Sharifkhodaei et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of either Dad^EP3196 or tkv^DN.Scer\UAS enhances the migration of wing disc cells expressing Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU into the non-expressing side of the disc.

Co-expression of two copies of mir-184^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} enhances the migration of wing disc cells expressing Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU into the non-expressing side of the disc.

Co-expression of either mir-184^Scer\UAS.cIa or tkv^{QD.Scer\UAS.cUa} suppresses the migration of wing disc cells expressing Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU into the non-expressing side of the disc.

The migration of wing disc cells expressing Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU into the non-expressing side of the disc is completely blocked by one copy of Dad^j1E4.

(Sharifkhodaei et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^da.PU/Gli^UAS.cSa partially rescues Gli^AE2Δ45

(Schulte et al., 2006)

Gli^UAS.cSa/Scer\GAL4^da.G32 partially rescues Gli^AE2Δ45

(Schulte et al., 2003)

Fails to rescue

Gli^UAS.cSa/Scer\GAL4^repo fails to rescue Gli^AE2Δ45

(Schulte et al., 2003)

Comments

Expression of Gli^Scer\UAS.cSa under the control of Scer\GAL4^da.PU fully rescues the embryonic lethality of Gli^AE2Δ45 homozygotes. 58% of the rescued adults have leg defects.

(Schulte et al., 2006)

The Gli^AE2Δ45 mutant phenotype and lethality can be rescued by expression of Gli^Scer\UAS.cSa under the regulation of Scer\GAL4^da.G32. 78% of rescued embryos hatch and survive to adulthood. Rescued adults are fertile and can be maintained as a stable stock, however, 58% have severe leg defects. The metatarsus and tibia are typically bent at 45^o toward the midline, and necrotic tissue is often present on the medial aspect of the limbs and limb joints. The lethality of homozygous Gli^AE2Δ45 mutants cannot be rescued by expression of Gli^Scer\UAS.cSa under the regulation of Scer\GAL4^repo.

(Schulte et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gli^Scer\UAS.cSa

Gli^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schulte

(Schulte et al., 2003)

Secondary FlyBase IDs

References (3)

Report Sections

Open Close