FlyBase Allele Report: Dmel\Gαq[UAS.cRa]

General Information

Symbol

Dmel\Gαq^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Ratnaparkhi

FlyBase ID

FBal0183499

Feature type

allele

Associated gene

Dmel\Gαq

Associated Insertion(s)

Carried in Construct

P{UAS-Gαq.R}

Also Known As

UAS-Gαq

Key Links

Transgenic product class

cDNA

(Ratnaparkhi et al., 2002)

wild_type

(Ratnaparkhi et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ratnaparkhi et al., 2002)

wild_type

(Ratnaparkhi et al., 2002)

Mutagen

in vitro construct

(Ratnaparkhi et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Gαq.R}

Cytology

Description

UASt regulatory sequences drive expression of the wild-type Gαq 3 splice variant cDNA.

(Ratnaparkhi et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gαq

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Detailed Description

Statement

Reference

Gα49B^Scer\UAS.cRa; Scer\GAL4^Ddc.PL flies a have slightly defective wing posture.

(Banerjee et al., 2006)

In embryos expressing Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155, the pattern of the central nervous system fascicles and commissures appears normal, when visualised with BP102. Overexpression of Gα49B^Scer\UAS.cRa under the regulation of Scer\GAL4^elav-C155 has no discernible effect on the embryonic stage central nervous system neuronal projection pattern, when visualised with Fas2.

(Ratnaparkhi et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Gαq^UAS.cRa/Scer\GAL4^Ddc.PL is a suppressor | partially of flightless | partially phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Gαq^UAS.cRa/Scer\GAL4^Ddc.PL is a suppressor | partially of abnormal neurophysiology phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Other

Statement

Reference

Df(2L)dp-79b, Dp(2;2)dpp^d21, Gαq^UAS.cRa, Gr21a^UAS.cKa, Gr63a^UAS.cKa, Scer\GAL4^Or22a.8010 has abnormal sensory perception phenotype

(Yao and Carlson, 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Gαq^UAS.cRa/Scer\GAL4^Ddc.PL is a suppressor | partially of wing phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Other

Statement

Reference

Df(2L)dp-79b, Dp(2;2)dpp^d21, Gαq^UAS.cRa, Gr21a^UAS.cKa, Gr63a^UAS.cKa, Scer\GAL4^Or22a.8010 has adult olfactory receptor neuron Or22 phenotype

(Yao and Carlson, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gr21a^Scer\UAS.cKa and Gr63a^Scer\UAS.cKa under the control of Scer\GAL4^Or22a.8010 in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dpp^d21 + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[[2]] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of Gα49B^Scer\UAS.cRa dramatically increases the response of the ab3A ORN to CO[[2]], to a level comparable to that of the endogenous CO[[2]] responsive neuron (ab1C).

(Yao and Carlson, 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Gr21a.9.323/Gαq^UAS.cRa rescues Gαq^RNAi.UAS

(Yao and Carlson, 2010)

Scer\GAL4^Gr21a.9.323/Gαq^UAS.cRa rescues Gαq^RNAi.UAS.1f1

(Yao and Carlson, 2010)

Scer\GAL4^{Orco.2.642.Hsim\VP22}/Gαq^UAS.cRa rescues Gαq^221c

(Kain et al., 2008)

Scer\GAL4^{Orco.2.642.Hsim\VP22}/Gαq^UAS.cRa rescues Gαq¹³⁷⁰

(Kain et al., 2008)

Comments

Co-expression of Gα49B^Scer\UAS.cRa restores a normal response of the ab1C olfactory receptor neuron to CO[[2]] in animals expressing either Gα49B^{dsRNA.Scer\UAS} or Gα49B^{dsRNA.Scer\UAS.1f1} under the control of Scer\GAL4^Gr21a.9.323.

(Yao and Carlson, 2010)

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B^221c or Gα49B¹³⁷⁰ homozygous clones in the antenna.

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} only during adulthood (expression is inhibited before this stage by expression of Scer\GAL80^ts.αTub84B at 18[o]C, and the animals are shifted to 29[o]C to inactivate Scer\GAL80^ts.αTub84B a few hours before eclosion) also rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B^221c or Gα49B¹³⁷⁰ homozygous clones in the antenna.

(Kain et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30734

w^*; P{UAS-Gαq.R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gα49B^Scer\UAS.cRa

Gαq^Scer\UAS.cRa

Gαq^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Ratnaparkhi

(Ratnaparkhi et al., 2002)

Secondary FlyBase IDs

References (10)

Report Sections

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