FlyBase Allele Report: Dmel\Gαq[1370]

General Information

Symbol

Dmel\Gαq¹³⁷⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0243256

Feature type

allele

Associated gene

Dmel\Gαq

Associated Insertion(s)

Carried in Construct

Also Known As

dgq¹³⁷⁰

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kain et al., 2008)

Progenitor genotype

Cytology

Description

Amino acid replacement: R207K.

(Kain et al., 2008)

Nucleotide substitution: G1933A.

(Kain et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:12,617,827..12,617,827 [+]

Nucleotide change:

G12617827A

Reported nucleotide change:

G1933A

Amino acid change:

Reported amino acid change:

R207K

(Kain et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | dominant

(Kain et al., 2008)

abnormal neurophysiology | somatic clone

(Kain et al., 2008)

lethal (with Df(2R)vg-C)

(Kain et al., 2008)

lethal (with Gαq^221c)

(Kain et al., 2008)

lethal | recessive

(Brusich et al., 2015)

lethal - all die before end of larval stage | recessive

(Kain et al., 2008)

some die during larval stage | recessive

(Kain et al., 2008)

viable (with Gαq¹)

(Kain et al., 2008)

Phenotype Manifest In

adult olfactory receptor neuron Or59b | somatic clone

(Kain et al., 2008)

Detailed Description

Statement

Reference

Gαq¹³⁷⁰/+ mutant larvae show a small decrease in quantal size. A compensatory increase in quantal content means that evoked neurotransmission is unaffected.

(Brusich et al., 2015)

Homozygotes die as either first or early second instar larvae.

Animals containing large homozygous clones in the antenna have normal numbers of trichoidea, coeloconica, and basiconica sensilla. The morphology and axonal projections of olfactory sensory neurons appear normal in these animals.

The electroantennograms of animals containing large homozygous clones in the antenna have a reduced amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

The electroantennograms of Gα49B¹³⁷⁰/+ animals show a small but significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

The response to ethyl acetate of ab2 large basiconic hairs that are located within homozygous clones in the antenna is significantly different from that of control cells; the firing frequency of the mutant ab2a neuron in response to ethyl acetate is strongly reduced compared to controls.

Gα49B¹/Gα49B¹³⁷⁰ flies do not show any defects in electroantennogram recordings.

(Kain et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gαq¹³⁷⁰ has abnormal neurophysiology | dominant phenotype, enhanceable by Plc21C[+]/Plc21C^A

(Kain et al., 2008)

Gαq¹³⁷⁰ has abnormal neurophysiology | dominant phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Kain et al., 2008)

Enhancer of

Statement

Reference

Gα49B[+]/Gαq¹³⁷⁰ is an enhancer of abnormal neurophysiology | dominant phenotype of Plc21C^k31911

(Kain et al., 2008)

Gα49B[+]/Gαq¹³⁷⁰ is an enhancer of abnormal neurophysiology | dominant phenotype of Plc21C^A

(Kain et al., 2008)

Suppressor of

Statement

Reference

Gα49B[+]/Gαq¹³⁷⁰ is a suppressor of abnormal neurophysiology phenotype of rdgA¹

(Kain et al., 2008)

Gα49B[+]/Gαq¹³⁷⁰ is a suppressor of abnormal neurophysiology phenotype of rdgA³

(Kain et al., 2008)

Other

Statement

Reference

Gαq¹³⁷⁰/Galphaq[+], GluRIIA^SP16 has abnormal neurophysiology phenotype

(Brusich et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Gαq¹³⁷⁰ partially prevents the compensatory increase in quantal content seen in the NMJs of GluRIIA^SP16 mutant larvae with reduced quantal size, resulting in impaired evoked neurotransmission. These is some increase in quantal content compared with Gαq¹³⁷⁰/+ controls, but not enough to compensate for the reduced quantal size.

(Brusich et al., 2015)

The electroantennograms of Gα49B¹³⁷⁰/Plc21C^A and Gα49B¹³⁷⁰/Plc21C^k31911 double heterozygous animals show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate) and the reduction in the double heterozygotes is significantly greater than that expected to arise from a mere additive effect of the single heterozygous phenotypes.

Introduction of a single copy of Gα49B¹³⁷⁰ to rdgA¹ or rdgA³ homozygotes significantly rescues the electroantennogram responses of the homozygotes.

(Kain et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Gαq^221c

(Kain et al., 2008)

Rescued by

Gαq¹³⁷⁰ is rescued by Scer\GAL4^{Orco.2.642.Hsim\VP22}/Gαq^UAS.cRa

(Kain et al., 2008)

Comments

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B¹³⁷⁰ homozygous clones in the antenna.

Expression of Gα49B^Scer\UAS.cRa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} only during adulthood (expression is inhibited before this stage by expression of Scer\GAL80^ts.αTub84B at 18[o]C, and the animals are shifted to 29[o]C to inactivate Scer\GAL80^ts.αTub84B a few hours before eclosion) also rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B¹³⁷⁰ homozygous clones in the antenna.

(Kain et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30736

Gαq¹³⁷⁰/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gα49B¹³⁷⁰

Gαq¹³⁷⁰

(Brusich et al., 2015)

dgq¹³⁷⁰

(Kain et al., 2009, Kain et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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