FB2026_02 , released June 18, 2026
Allele: Dmel\Pcyt1Ala.UAS
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General Information
Symbol
Dmel\Pcyt1Ala.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0184377
Feature type
allele
Associated gene
Dmel\Pcyt1
Associated Insertion(s)
Carried in Construct
P{UAS-Pcyt1.Ala}
Key Links
Transgenic product class
dominant_negative_variant
(Weber et al., 2003)
missense_variant
(Weber et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Weber et al., 2003)
missense_variant
(Weber et al., 2003)
Mutagen
in vitro construct
(Weber et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Pcyt1.Ala}
Cytology
Description

UASt sequences drive expression of a mutated form of Pcyt1 (carries the amino acid replacements H218A and H221A).

(Weber et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pcyt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Cct1Ala.Scer\UAS, driven by Scer\GAL4sd-SG29.1, causes extra crossveins to form at the tip of L2 in the wing in >30% of flies.

      (Weber et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Pcyt1Ala.UAS, Scer\GAL4sd-SG29.1 has crossvein | increased number phenotype, enhanceable by Egfr[+]/Egfrt1

      (Weber et al., 2003)

      Pcyt1Ala.UAS, Scer\GAL4sd-SG29.1 has crossvein | increased number phenotype, enhanceable by Egfr[+]/Egfrtop-18A

      (Weber et al., 2003)
      Suppressor of
      Statement
      Reference

      Scer\GAL4sd-SG29.1/Pcyt1Ala.UAS is a suppressor of crossvein | increased number phenotype of EgfrE3

      (Weber et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The extra crossvein wing phenotype of Cct1Ala.Scer\UAS mutants is enhanced by the removal of one copy of Egfr, showing an increasing effect with stronger alleles. Thus, expression of Cct1Ala.Scer\UAS (driven by Scer\GAL4sd-SG29.1) in Egfrtop-18A/+ flies causes wings with more extra crossveins than expression in Egfrt1/+ flies; Egfrt1 is a weak Egfr allele. EgfrE3 mutant flies in which Cct1Ala.Scer\UAS is expressed under the control of Scer\GAL4sd-SG29.1, have a smaller average number of ectopic crossveins in the wing than EgfrE3 single mutants.

      (Weber et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CCT1Ala
      (Weber et al., 2003)
      Cct1Ala.Scer\UAS
      Pcyt1Ala.Scer\UAS
      Pcyt1Ala.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)