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General Information
Symbol
Dmel\aPKCDN.UAS.Tag:CAAX(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0189627
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a membrane-targeted (by the CAAX motif) kinase-dead aPKC sequence. Carries the amino acid replacement K293W.

UASt regulatory sequences drive expression of a dominant-negative, kinase-dead form of aPKC (carries a K293W amino acid substitution in the ATP binding site) that is targeted to the membrane due to the presence of a Tag:CAAX(Unk) tag.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 has no effect on growth or survival of the wing discs.

Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4wor.PA has no affect on the number of neuroblasts in the larval brain.

Overexpression of aPKCCAAXDN.Scer\UAS, under the control of Scer\GAL4en-e16E results in the loss of membrane associated crb and Patj concomitant with an apical expansion of basolateral markers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 suppresses the tumorous growth seen in slmbUU11 mutant wing disc clones. The resulting clones are smaller than in wild type.

Coexpression of aPKCCAAXDN.Scer\UAS partially suppresses both the polarity and proliferation phenotypes of wing discs expressing Syx7dsRNA.Scer\UAS.cLa under the control of Scer\GAL4en-e16E.

Coexpression of aPKCCAAXDN.Scer\UAS partially suppresses the early pupal lethality associated with expression of Syx7dsRNA.Scer\UAS.cLa under the control of Scer\GAL4en-e16E, increasing survival of pharate adults from 30% to 91%.

Expression of kinase-dead aPKCCAAXDN.Scer\UAS, under the regulation of Scer\GAL4en-e16E largely normalises the effect caused by overexpression of wild-type crbintra.Scer\UAS (under the control of Scer\GAL4en-e16E). Expression of kinase-dead aPKCCAAXDN.Scer\UAS, under the regulation of Scer\GAL4en-e16E normalises the effect caused by overexpression of wild-type crbT6DT9D.intra.Scer\UAS (under the control of Scer\GAL4en-e16E) in approximately 40% of the flies, even though these flies still display strong cuticular defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
aPKCCAAXDN.Scer\UAS
aPKCCAAXDN.UAS
aPKCDN.UAS.Tag:CAAX(Unk)
Name Synonyms
Secondary FlyBase IDs
    References (7)