FlyBase Allele Report: Dmel\crb[intra.UAS]

Expression of crb^{intra.Scer\UAS} under the control of Scer\GAL4^GMR.PF of Scer\GAL4^sev.EP.M results in a rough eye phenotype, characterised by perturbations of the regular pattern of ommatidia and occasional bristle duplications. In pupal eyes that are expressing crb^{intra.Scer\UAS} under the control of Scer\GAL4^GMR.PF, the number of interommatidial (lattice) cells (IOCs) is increased compared to wild type, and sorting of these cells fails to occur in many instances. Many of the surplus IOCs are aligned side-to-side instead of end-to-end, so that two cell rows are found between many ommatidial cell clusters. The secondary and tertiary pigment cells are often arranged "astrally", with four cells (instead of the usual three) surrounding a bristle cell. This results in the ommatidia adopting a square rather than a hexagonal shape. Larval eye imaginal discs expressing crb^{intra.Scer\UAS} under the control of Scer\GAL4^GMR.PF show a strong reduction in the number of apoptotic cells posterior to the morphogenetic furrow. In pupal eye discs, however, suppression of apoptosis is less pronounced and is only visible in small patches in the disc.

(Grzeschik and Knust, 2005)

The degree of wing disc overgrowth caused by expression of crb^{intra.Scer\UAS} under the control of Scer\GAL4^en-e16E increases as transgene expression levels are elevated.

(Lu and Bilder, 2005)

Overexpression of crb^{intra.Scer\UAS}, under the regulation of Scer\GAL4^en-e16E results in severe tissue disorganisation in the wing, which is attributed to 'apicalisation' of the wing disc cells and causes strong cuticular defects.

(Sotillos et al., 2004)

Flies expressing crb^{intra.Scer\UAS} under the control of Scer\GAL4^GMR.PF have reduced eyes which have an irregular external morphology and bristle pattern.

(Tanentzapf and Tepass, 2003)

Overexpression of crb results in an expansion of the apical membrane domain accompanied by a reduction of the basolateral membrane domain. The amnioserosa cells lack apical zonula adherens junctions (ZA), spot adherens junctions (SAJ) are also abnormal.

(Grawe et al., 1996)

Phenotype is exactly as for crb^Scer\UAS.cWa, when driven by the same Scer\GAL4 lines.

(Wodarz et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, crb^intra.UAS has visible phenotype, enhanceable by slmb[+]/slmb^UU11

(Skwarek et al., 2014)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by Df(2L)DV271

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by scrib[+]/scrib⁶⁷³

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by dlg1[+]/dlg1¹⁴

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by l(2)gl[+]/l(2)gl⁰¹⁴³³

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by l(2)gl²⁷⁵

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has visible phenotype, enhanceable by l(2)gl[+]/l(2)gl⁴

(Tanentzapf and Tepass, 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, crb^intra.UAS has visible | adult stage phenotype, suppressible by Ciao1^GD7831, Scer\GAL4^GMR.PU

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has decreased size | adult stage phenotype, suppressible by Ciao1^GD7831, Scer\GAL4^GMR.PU

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has visible | adult stage phenotype, suppressible by Ciao1^Δ60/Ciao1[+]

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has decreased size | adult stage phenotype, suppressible by Ciao1^Δ60/Ciao1[+]

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has visible phenotype, suppressible by ex^UAS.cUa/Scer\GAL4^GMR.PU

(Chung et al., 2016)

Scer\GAL4^GMR.PU, crb^intra.UAS has visible phenotype, suppressible by Schip1^UAS.cCa/Scer\GAL4^GMR.PU

(Chung et al., 2016)

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, suppressible by veli^RNAi.UAS.cBa/Scer\GAL4^rn-GAL4-5

(Andersen et al., 2015)

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, suppressible by veli⁶⁶

(Andersen et al., 2015)

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, suppressible by grnd^KK109939/Scer\GAL4^rn-GAL4-5

(Andersen et al., 2015)

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, suppressible by grnd^MI05292

(Andersen et al., 2015)

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^rn-GAL4-5

(Andersen et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^rn-GAL4-5, crb^intra.UAS has neoplasia phenotype, non-suppressible by wgn^KO

(Andersen et al., 2015)

Suppressor of

Statement

Reference

crb^intra.UAS/Scer\GAL4^en.PU is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^en.PU, ex^UAS.cFa

(Fulford et al., 2019)

crb^intra.UAS/Scer\GAL4^en.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^en.PU, ex^UAS.cFa

(Fulford et al., 2019)

NOT Suppressor of

Statement

Reference

crb^intra.UAS/Scer\GAL4^en.PU is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^en.PU, ex^S453A.UAS

(Fulford et al., 2019)

crb^intra.UAS/Scer\GAL4^en.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^en.PU, ex^S453A.UAS

(Fulford et al., 2019)

Other

Statement

Reference

Df(2L)DV271, Scer\GAL4^en-e16E, crb^intra.UAS has lethal phenotype

(Tanentzapf and Tepass, 2003)

Scer\GAL4^en-e16E, crb^intra.UAS, l(2)gl²⁷⁵ has lethal phenotype

(Tanentzapf and Tepass, 2003)

Scer\GAL4^en-e16E, crb^intra.UAS, l(2)gl⁴ has lethal phenotype

(Tanentzapf and Tepass, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, crb^intra.UAS has eye phenotype, enhanceable by slmb[+]/slmb^UU11

(Skwarek et al., 2014)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by Df(2L)DV271

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by l(2)gl[+]/l(2)gl⁴

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by scrib[+]/scrib⁶⁷³

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by dlg1[+]/dlg1¹⁴

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by l(2)gl[+]/l(2)gl⁰¹⁴³³

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has wing phenotype, enhanceable by l(2)gl[+]/l(2)gl⁰¹⁴³³

(Tanentzapf and Tepass, 2003)

Scer\GAL4^GMR.PF, crb^intra.UAS has eye phenotype, enhanceable by l(2)gl²⁷⁵

(Tanentzapf and Tepass, 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, crb^intra.UAS has photoreceptor cell R8 phenotype, suppressible by yki^KK109756/Scer\GAL4^GMR.long

(Pojer et al., 2021)

Scer\GAL4^GMR.PU, crb^intra.UAS has eye phenotype, suppressible by Ciao1^GD7831, Scer\GAL4^GMR.PU

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has eye phenotype, suppressible by Ciao1^Δ60/Ciao1[+]

(Jung et al., 2020)

Scer\GAL4^GMR.PU, crb^intra.UAS has eye phenotype, suppressible by ex^UAS.cUa/Scer\GAL4^GMR.PU

(Chung et al., 2016)

Scer\GAL4^GMR.PU, crb^intra.UAS has eye phenotype, suppressible by Schip1^UAS.cCa/Scer\GAL4^GMR.PU

(Chung et al., 2016)

Scer\GAL4^en-e16E, crb^intra.UAS has dorsal mesothoracic disc & epithelial cell phenotype, suppressible by Scer\GAL4^en-e16E/aPKC^{DN.UAS.Tag:CAAX(Unk)}

(Sotillos et al., 2004)

Suppressor of

Statement

Reference

crb^intra.UAS/Scer\GAL4^en.PU is a suppressor | partially of wing posterior compartment phenotype of Scer\GAL4^en.PU, ex^UAS.cFa

(Fulford et al., 2019)

crb^intra.UAS/Scer\GAL4^btl.PS is a suppressor | partially of presumptive embryonic salivary gland phenotype of rib^P7/rib¹

(Kerman et al., 2008)

NOT Suppressor of

Statement

Reference

crb^intra.UAS/Scer\GAL4^en.PU is a non-suppressor of wing posterior compartment phenotype of Scer\GAL4^en.PU, ex^S453A.UAS

(Fulford et al., 2019)

crb^intra.UAS/Scer\GAL4^btl.PS is a non-suppressor of tracheal dorsal trunk primordium phenotype of rib^P7/rib¹

(Kerman et al., 2008)

Other

Statement

Reference

Scer\GAL4^GMR.long, crb^intra.UAS, yki^KK109756 has photoreceptor cell R8 phenotype

(Pojer et al., 2021)

Scer\GAL4^da.G32, baz^{S980A.UAS.GFP}, crb^intra.UAS has embryonic/first instar larval cuticle phenotype

(Krahn et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of yki^KK109756 suppresses the increased ratio of pale vs yellow photoreceptor cells R8 induced by the expression of crb^intra.UAS under the control of Scer\GAL4^GMR.long, even leading to all photoreceptor cells R8 becoming the yellow type.

(Pojer et al., 2021)

Co-expression of ex^Scer\UAS.cUa or Schip1^Scer\UAS.cCa suppresses the small rough eye phenotype in flies with expression of crb^{intra.Scer\UAS} driven by Scer\GAL4^GMR.PU.

(Chung et al., 2016)

One copy of slmb^UU11 enhances the eye phenotypes seen when crb^{intra.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

(Skwarek et al., 2014)

Expression of crb^{intra.Scer\UAS} and baz^{S980A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 results in the same phenotype as overexpression of baz^{S980A.Scer\UAS.T:Avic\GFP} alone. Only little scraps of embryonic cuticule are observed.

(Krahn et al., 2010)

Expression of crb^{intra.Scer\UAS} in the trachea under the control of Scer\GAL4^btl.PS in rib¹/rib^P7 mutant animals partially rescues the tracheal dorsal trunk extension defects.

Expression of crb^{intra.Scer\UAS} in the salivary gland under the control of Scer\GAL4^fkh.PH in rib¹/rib^P7 animals fails to rescue salivary gland migration defects.

(Kerman et al., 2008)

Expression of kinase-dead aPKC^{CAAXDN.Scer\UAS}, under the regulation of Scer\GAL4^en-e16E largely normalises the effect caused by overexpression of wild-type crb^{intra.Scer\UAS} (under the control of Scer\GAL4^en-e16E).

(Sotillos et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600578

w^*; P{UAS-crb.intra}2/CyO, P{Dfd-EYFP.w^BmC}2; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

crb^{intra.Scer\UAS}

crb^intra.UAS

crb^intra

(Lodge et al., 2021, Chung et al., 2016, Wodarz et al., 1995)

Name Synonyms

Secondary FlyBase IDs

References (30)

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