FlyBase Allele Report: Dmel\slmb[UU11]

General Information

Symbol

Dmel\slmb^UU11

Species

D. melanogaster

Name

FlyBase ID

FBal0284276

Feature type

allele

Associated gene

Dmel\slmb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W147term.

(Skwarek et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,122,434..21,122,434 [-]

Nucleotide change:

G21122434A

Amino acid change:

W147term | slmb-PA; W147term | slmb-PB; W147term | slmb-PC

Reported amino acid change:

W147term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Skwarek et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

neoplasia | somatic clone

(Skwarek et al., 2014)

Phenotype Manifest In

chromatin | somatic clone

(Buster et al., 2013)

neuroblast | somatic clone

(Skwarek et al., 2014)

wing disc | somatic clone

(Skwarek et al., 2014)

Detailed Description

Statement

Reference

Neoplastic transformation is seen in slmb^UU11 mutant wing discs and slmb^UU11 mutant eye discs fail to differentiate. slmb^UU11 mutant wing disc clones grow larger than wild type controls. slmb^UU11 mutant neuroblast clones display defects in asymmetric cell division.

(Skwarek et al., 2014)

Chromatin is reorganised into compact, spherical structures surrounded by distorted nuclear envelopes in homozygous clones in the wing disc.

(Buster et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

slmb^UU11 has neoplasia | somatic clone phenotype, suppressible by yki^B5

(Skwarek et al., 2014)

slmb^UU11 has neoplasia | somatic clone phenotype, suppressible by aPKC^{DN.UAS.Tag:CAAX(Unk)}/Scer\GAL4^Bx-MS1096

(Skwarek et al., 2014)

Enhancer of

Statement

Reference

slmb[+]/slmb^UU11 is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, crb^intra.UAS

(Skwarek et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

slmb^UU11 has wing disc | somatic clone phenotype, suppressible by yki^B5

(Skwarek et al., 2014)

slmb^UU11 has wing disc | somatic clone phenotype, suppressible by aPKC^{DN.UAS.Tag:CAAX(Unk)}/Scer\GAL4^Bx-MS1096

(Skwarek et al., 2014)

Enhancer of

Statement

Reference

slmb[+]/slmb^UU11 is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, crb^intra.UAS

(Skwarek et al., 2014)

slmb[+]/slmb^UU11 is an enhancer of polytene chromosome phenotype of Cap-H2^Z3-0019, glu[+]/glu^k08819

(Buster et al., 2013)

slmb[+]/slmb^UU11 is an enhancer of nurse cell phenotype of Cap-H2^Z3-0019, glu[+]/glu^k08819

(Buster et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

yki^B5 suppresses the neoplastic transformation seen in slmb^UU11 mutant wing discs.

One copy of slmb^UU11 enhances the eye phenotypes seen when crb^{intra.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of aPKC^{CAAXDN.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 suppresses the tumorous growth seen in slmb^UU11 mutant wing disc clones. The resulting clones are smaller than in wild type.

(Skwarek et al., 2014)

glu^k08819/+ ; Cap-H2^Z3-0019/+ double heterozygotes show some unpairing of polytene chromosomes in nurse cells. Unpairing is significantly increased if the animals are also heterozygous for either slmb^UU11.

(Buster et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

slmb^UU11 is rescued by slmb^{αTub84B.Tag:MYC}

(Skwarek et al., 2014)

Comments

Expression of slmb^{αTub84B.T:Hsap\MYC} rescues the neoplastic phenotype seen in slmb^UU11 mutant wing discs.

(Skwarek et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65424

w^*; P{neoFRT}82B slmb^UU11/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

UU11

(Skwarek et al., 2014)

slimb^UU11

(Buster et al., 2013)

slmb^UU11

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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