FlyBase Allele Report: Dmel\pad[1]

General Information

Symbol

Dmel\pad¹

Species

D. melanogaster

Name

FlyBase ID

FBal0189942

Feature type

allele

Associated gene

Dmel\pad

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

natural population

Nature of the Allele

Allele class

Mutagen

natural population

(Gibert et al., 2005)

Progenitor genotype

Cytology

Description

Deletion in the third exon of 29bp from position 976 to 1004 downstream of the A in the predicted ATG. Induces a frameshift and introduces 20 new codons followed by a stop codon. The predicted truncated protein would be 308 amino acid residues long and would lack the four zinc fingers located in the C-terminal part.

(Gibert et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile | recessive

(Gibert et al., 2005)

viable | recessive

(Gibert et al., 2005)

Phenotype Manifest In

anterior scutellar bristle | female | increased number

(Gibert et al., 2005)

mesothoracic tergum & macrochaeta | ectopic

(Gibert et al., 2005)

mesothoracic tergum & macrochaeta | ectopic (with Df(3R)sbd26)

(Gibert et al., 2005)

mesothoracic tergum & macrochaeta | ectopic (with Tp(3;1)P115)

(Gibert et al., 2005)

mesothoracic tergum & microchaeta

(Gibert et al., 2005)

posterior postalar bristle | female | increased number

(Gibert et al., 2005)

posterior supraalar bristle | female | increased number

(Gibert et al., 2005)

presutural bristle

(Gibert et al., 2005)

sensillum campaniformium & wing | supernumerary

(Gibert et al., 2005)

Detailed Description

Statement

Reference

In pad¹ mutants, the number of dorso-central macrochaetes is increased; the effect is greater in females (~13 ectopic bristles) than males (~9 ectopic bristles). The anterior scutellar, posterior post-alar and posterior supra-alar bristles are frequently duplicated in females. The presutural bristles are bigger than wild type and are the size of macrochaetes. Additional bristles are sometimes found laterally, and are usually shorter and thicker than wild type. The density of microchaetes is also increased. Transheterozygous pad¹/Df(3R)sbd26 and pad¹/Tp(3;1)P115 flies also display an ectopic bristle phenotype. pad¹ heterozygotes display a wild-type bristle pattern. pad¹ mutants often have four or five sensilla campaniformia on the third vein of the wing, instead of three. There are often three sensilla at the location of the twin sensilla of the anterior wing margin.

(Gibert et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, enhanceable by hry¹

(Gibert et al., 2005)

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, enhanceable by tkv¹

(Gibert et al., 2005)

Suppressed by

Statement

Reference

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, suppressible by sc^bald

(Gibert et al., 2005)

NOT suppressed by

Statement

Reference

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, non-suppressible by Df(1)115

(Gibert et al., 2005)

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, non-suppressible by Df(1)91B

(Gibert et al., 2005)

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, non-suppressible by In(1)ac³

(Gibert et al., 2005)

pad¹ has mesothoracic tergum & macrochaeta | ectopic phenotype, non-suppressible by In(1)sc⁴

(Gibert et al., 2005)

Enhancer of

Statement

Reference

pad¹ is an enhancer of mesothoracic tergum & macrochaeta | ectopic phenotype of hry¹

(Gibert et al., 2005)

pad¹ is an enhancer of mesothoracic tergum & macrochaeta | ectopic phenotype of tkv¹

(Gibert et al., 2005)

Other

Statement

Reference

pad¹, put[+]/put¹⁰⁴⁶⁰ has dorsocentral bristle | ectopic phenotype

(Gibert et al., 2005)

pad¹, put¹⁰⁴⁶⁰ has dorsocentral bristle | ectopic phenotype

(Gibert et al., 2005)

pad[+]/pad¹, put¹⁰⁴⁶⁰ has dorsocentral bristle | ectopic phenotype

(Gibert et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The sc^bald mutation suppresses the ectopic bristle phenotype of pad¹ flies, while the following mutations do not suppress this phenotype: In(1)ac³, Df(1)91B, Df(1)115 and In(1)sc⁴. pad¹ h¹ double mutants raised at 25^oC display ectopic bristles at positions on the notum where no ectopic bristles are found in either single mutant. These new positions include dorso-central bristles closer to the thoracic midline and additional bristles between the anterior and posterior scutellars. Ectopic bristles on the double mutant are mostly located in the posterior half of the notum, while ectopic bristles tend to be found in the anterior half in pad¹ single mutants. Transheterozygous put¹⁰⁴⁶⁰ pad¹ flies have ectopic dorso-central bristles, while neither single heterozygote shows this phenotype. tkv¹ pad¹ double mutants show an enhanced ectopic bristle phenotype compared to either single mutant; there are particularly more ectopic bristles present around the presutal suture.