FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Gcn5E333st
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General Information
Symbol
Dmel\Gcn5E333st
Species
D. melanogaster
Name
FlyBase ID
FBal0190225
Feature type
allele
Associated gene
Dmel\Gcn5
Associated Insertion(s)
Carried in Construct
Also Known As
PcafE333st, dGcn5E333st
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Carre et al., 2005)
Progenitor genotype
Cytology
Description

Amino acid replacement: E333term.

(Carre et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:12,522,907..12,522,907 [-]
Nucleotide change:

G12522907T

Amino acid change:

E333term | Gcn5-PA

Reported amino acid change:

E333term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Carre et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  familial nephrotic syndrome
      CEA
      (Gonçalves et al., 2018)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Huntington's disease
      modeled by Hsap\HTTQ93.ex1.UAS
      (Bodai et al., 2012)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult cuticle & adult abdomen (with Gcn5C137Y)

      (Carre et al., 2005)

      adult cuticle & adult abdomen (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      adult heart (with Df(3L)sex204), with Gcn5F304S.UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      adult heart (with Df(3L)sex204), with Gcn5UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      adult pericardial cell (with Df(3L)sex204)

      (Gonçalves et al., 2018)

      adult pericardial cell (with Df(3L)sex204), with Gcn5F304S.UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      adult pericardial cell (with Df(3L)sex204), with Gcn5UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      cuticle & abdomen & pharate adult (with Gcn5C137Y)

      (Carre et al., 2005)

      cuticle & abdomen & pharate adult (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      embryonic/larval neuromuscular junction | larval stage (with Gcn5Q186st)

      (Wang et al., 2020)

      eye (with Df(3L)sex204), with Gcn5F304S.UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      eye (with Df(3L)sex204), with Gcn5ΔGcn5.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      eye (with Gcn5C137Y)

      (Carre et al., 2005)

      eye (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      eye photoreceptor cell

      (Weake et al., 2008)

      femur (with Df(3L)sex204), with Gcn5ΔGcn5.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      imaginal disc

      (Carre et al., 2005)

      leg (with Df(3L)sex204), with Gcn5F304S.UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      metathoracic leg (with Gcn5C137Y)

      (Carre et al., 2005)

      metathoracic leg (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      metathoracic leg | pharate adult stage (with Gcn5C137Y)

      (Carre et al., 2005)

      metathoracic leg | pharate adult stage (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      ommatidium (with Gcn5C137Y)

      (Carre et al., 2005)

      ommatidium (with Gcn5ΔT280-F285)

      (Carre et al., 2005)

      oocyte | oogenesis stage S5 (with Df(3L)sex204), with Gcn5UAS.cCa, Scer\GAL4da.G32

      (Carre et al., 2005)

      oocyte | oogenesis stage S5 (with Df(3L)sex204), with Gcn5ΔBromo.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      oocyte | oogenesis stage S6 (with Df(3L)sex204), with Gcn5UAS.cCa, Scer\GAL4da.G32

      (Carre et al., 2005)

      oocyte | oogenesis stage S6 (with Df(3L)sex204), with Gcn5ΔBromo.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      ovary (with Df(3L)sex204), with Gcn5UAS.cCa, Scer\GAL4da.G32

      (Carre et al., 2005)

      ovary (with Df(3L)sex204), with Gcn5ΔBromo.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      polytene chromosome puff | wandering third instar larval stage

      (Carre et al., 2005)

      prepupa

      (Carre et al., 2005)

      prepupa (with Gcn5Q186st)

      (Carre et al., 2005)

      wing (with Df(3L)sex204), with Gcn5F304S.UAS.Tag:FLAG, Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      wing (with Df(3L)sex204), with Gcn5ΔGcn5.UAS, Scer\GAL4da.G32

      (Carre et al., 2005)

      wing (with Gcn5C137Y)

      (Carre et al., 2005)

      wing (with Gcn5ΔT280-F285)

      (Carre et al., 2005)
      Detailed Description
      Statement
      Reference

      Gcn5E333st/Df(3L)sex204 transheterozygous larvae present morphologically normal nephrocytes. In adult escapers, pericardial nephrocytes are frequently abnormally-shaped.

      Gcn5E333st/Df(3L)sex204 transheterozygotes rescued to adulthood by the expression of Gcn5F304S.UAS.Tag:FLAG under the control of Scer\GAL4da.G32 are flightless and exhibit fully penetrant blistered wing and small, mildly rough eye phenotypes, and exhibit frequent leg morphology defects. They show cardiac defects: significant decreases in cardiac output, diastolic diameter and fractional shortening, and significant increases in heart period and arrhythmia index. They also show abnormally-shaped pericardial nephrocytes.

      Gcn5E333st/Df(3L)sex204 transheterozygotes rescued to adulthood by the expression of Gcn5UAS.Tag:FLAG under the control of Scer\GAL4da.G32 also show some heart defects: significant decreases in cardiac output and diastolic diameter, but no significant changes in heart period, fractional shortening and arrhythmia index. They also show abnormally-shaped pericardial nephrocytes.

      (Gonçalves et al., 2018)

      Photoreceptor organization is disrupted in PcafE333st eye discs and there is a defect in the R-cell projection pattern.

      (Weake et al., 2008)

      PcafE333st/PcafE333st and PcafE333st/PcafQ186st animals develop to wandering third instar, but do not form a puparium at the normal time and continue to wander for an additional 4-5 days. When they eventually stop moving, they fail to evert their spiracles and form abnormally long prepupae. These die with partial separation of the posterior part of the prepupa from the pupal case. PcafE333st/PcafE333st animals fail to form normal (for late third instar) sized early polytene chromosome puffs (2B, 74EF, and 75B) during their extended wandering third instar stage. Imaginal discs from PcafE333st homozygous third instar larvae are misshapen and severely reduced in size . PcafE333st/PcafC137T and PcafE333st/PcafΔT280-F285 animals form their puparium normally, but during pupation they fail to elongate their metathoracic legs correctly and to deposit some or all abdominal cuticle. Rare adult escapers have misshapen wings, rough eyes, and crooked and twisted metathoracic legs and die a few hours after eclosion. In PcafE333st/Df(3L)sex204 females rescued to adulthood by PcafScer\UAS.cCa or PcafΔBromo.Scer\UAS with Scer\GAL4da.G32, oogenesis is arrested at stage 5-6.

      (Carre et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Pcaf[+]/Gcn5E333st is an enhancer of ommatidium phenotype of Hsap\HTTQ93.ex1.UAS, Scer\GAL4elav-C155

      (Bodai et al., 2012)

      Pcaf[+]/Gcn5E333st is an enhancer of rhabdomere phenotype of Hsap\HTTQ93.ex1.UAS, Scer\GAL4elav-C155

      (Bodai et al., 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      One copy of PcafE333st enhances the reduction in eclosion rate seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155. These flies have a relative eclosion rate of 49.4%. PcafE333st/+ also enhances rhabdomere loss, with an average of 4.32 rhabdomeres per ommatidium.

      (Bodai et al., 2012)
      Complementation and Rescue Data
      Rescued by

      Df(3L)sex204/Gcn5E333st is rescued by Scer\GAL4da.G32/Gcn5UAS.Tag:FLAG

      (Gonçalves et al., 2018)

      Df(3L)sex204/Gcn5E333st is rescued by Scer\GAL4da.G32/Gcn5S478F.UAS.Tag:FLAG

      (Gonçalves et al., 2018)

      Gcn5E333st is rescued by Gcn5+t4.7

      (Carre et al., 2005)

      Df(3L)sex204/Gcn5E333st is rescued by Gcn5UAS.cCa/Scer\GAL4da.G32

      (Carre et al., 2005)

      Df(3L)sex204/Gcn5E333st is rescued by Gcn5ΔBromo.UAS/Scer\GAL4da.G32

      (Carre et al., 2005)

      Df(3L)sex204/Gcn5E333st is rescued by Gcn5+t4.7

      (Carre et al., 2005)
      Partially rescued by

      Df(3L)sex204/Gcn5E333st is partially rescued by Gcn5F304S.UAS.Tag:FLAG/Scer\GAL4da.G32

      (Gonçalves et al., 2018)

      Df(3L)sex204/Gcn5E333st is partially rescued by Gcn5UAS.Tag:FLAG/Scer\GAL4αTub84B.PL

      (Gonçalves et al., 2018)

      Df(3L)sex204/Gcn5E333st is partially rescued by Gcn5F304S.UAS.Tag:FLAG/Scer\GAL4αTub84B.PL

      (Gonçalves et al., 2018)

      Df(3L)sex204/Gcn5E333st is partially rescued by Gcn5S478F.UAS.Tag:FLAG/Scer\GAL4αTub84B.PL

      (Gonçalves et al., 2018)

      Df(3L)sex204/Gcn5E333st is partially rescued by Scer\GAL4da.G32/Gcn5ΔGcn5.UAS

      (Carre et al., 2005)

      Df(3L)sex204/Gcn5E333st is partially rescued by Gcn5ΔAda.UAS/Scer\GAL4da.G32

      (Carre et al., 2005)
      Not rescued by

      Df(3L)sex204/Gcn5E333st is not rescued by Gcn5ΔHAT.UAS/Scer\GAL4da.G32

      (Carre et al., 2005)
      Comments

      Expressing Gcn5F304S.UAS.Tag:FLAG under the control of either Scer\GAL4da.G32 or Scer\GAL4αTub84B.PL partially rescues the full lethality of Gcn5E333st/Df(3L)sex204, giving rise to a few or to nearly half of the expected adult progeny, respectively.

      (Gonçalves et al., 2018)

      PcafΔHAT.Scer\UAS; Scer\GAL4da.G32 fails to rescue the lethality or reduction in polytene chromosome acetylation due to PcafE333st/Df(3L)sex204. PcafΔAda.Scer\UAS; Scer\GAL4da.G32 rescues polytene chromosome acetylation, but not lethality do to PcafE333st/Df(3L)sex204.

      (Carre et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Gcn5E333st
      (Faragó et al., 2022, Gonçalves et al., 2018)
      Gcn5E333stop
      (Grau et al., 2008)
      PcafE333St
      (Bodai et al., 2012)
      PcafE333st
      (Kirilly et al., 2011, Weake et al., 2008)
      dGcn5E333st
      (Vamos and Boros, 2012, Kirilly et al., 2011)
      gcn5E333st
      (Wang et al., 2020, Liu et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)