FlyBase Allele Report: Dmel\pip[7]

General Information

Symbol

Dmel\pip⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0191371

Feature type

allele

Associated gene

Dmel\pip

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Zhu et al., 2005)

Progenitor genotype

Cytology

Description

Mutation is within the fourth pip-PA (Pipe-ST2) specific exon.

(Zhu et al., 2005)

Amino acid replacement: V123I.

(Zhu et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,300,116..19,300,116 [-]

Nucleotide change:

G19300116A

Amino acid change:

V133I | pip-PA

Reported amino acid change:

V123I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Difference between reported and FlyBase amino acid change due to authors use of GenBank record gi:7293803 which has since been replaced by a new version gi:28380469 .

(Zhu et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | maternal effect (with Df(3L)pipe^A13)

(Zhu et al., 2005)

embryonic/first instar larval cuticle | maternal effect (with pip²)

(Zhang et al., 2009)

Detailed Description

Statement

Reference

Embryos derived from pip²/pip⁷ females show a range of phenotypes ranging from weakly dorsalised (12.1%) to completely dorsalised (15.7%).

(Zhang et al., 2009)

pip⁷/Df(3L)pipe^A13 females produce weakly dorsalized embryos. When females are fed with 1M sodium chlorate, which inhibits PAPS synthetase activity, 94% of embryos show a completely dorsalized cuticular phenotype and apolar gastrulation phenotypes.

(Zhu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Vml^EY21650/Vml^EY21650

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Vml^EY21650/Vml[+]

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Df(2L)Exel7024/+

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Df(2L)Exel7024/Vml^EY21650/Vml[+]/+

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by gd^VM90/gd[+]

(Zhang et al., 2009)

NOT Enhanced by

Statement

Reference

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, non-enhanceable | maternal effect by dec-1[+]/dec^VA28

(Zhang et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Vml^EY21650/Vml^EY21650, such that 89.5% of the embryos derived from the double mutant females are completely dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Vml^EY21650/+; 48.0% of embryos derived from the double mutant females are completely dorsalised, and only 1.2% are weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Df(2L)Exel7024/+; 52.1% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Df(2L)Exel7024/+ and Vml^EY21650/+; 69.5% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is not increased if the females are also carrying dec-1^VA28/+.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying gd^VM90/+; 78.8% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

(Zhang et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

K. Anderson

(Zhu et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pip⁷

pipe⁷

(Zhang et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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