FlyBase Allele Report: Dmel\pip[2]

General Information

Symbol

Dmel\pip²

Species

D. melanogaster

Name

pipe²

FlyBase ID

FBal0013837

Feature type

allele

Associated gene

Dmel\pip

Associated Insertion(s)

Carried in Construct

Also Known As

pip⁶⁶⁴, pipe⁶⁶⁴, pipe²

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

G to A mutation within the 5' intron splicing site for intron 4 of the pip-RA transcript which encodes the pip-PA (Pipe-ST2) isoform.

(Zhu et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle field | maternal effect | somatic clone

(Zhu et al., 2005)

embryo | maternal effect | somatic clone

(Lachance et al., 2009)

embryonic/first instar larval cuticle

(Sen et al., 1998)

embryonic/first instar larval cuticle | maternal effect (with pip⁷)

(Zhang et al., 2009)

embryonic epidermis | maternal effect | somatic clone

(Zhu et al., 2005)

Detailed Description

Statement

Reference

Embryos derived from egg chambers containing complete homozygous follicle cell clones are completely dorsalised.

(Lachance et al., 2009)

Embryos derived from pip²/pip⁷ females show a range of phenotypes ranging from weakly dorsalised (12.1%) to completely dorsalised (15.7%).

(Zhang et al., 2009)

pip² homozygous females produce dorsalised embryos.

(Shapiro and Anderson, 2006)

Females carrying pip² mutant ventral follicle cells produce partially dorsalized embryos. This is indicated by a tail-up phenotype and narrow ventral denticle bands.

(Zhu et al., 2005)

Eggs from pip¹/pip² mothers do not have apparent vitelline membrane defects.

(LeMosy and Hashimoto, 2000)

Embryos derived from pip¹/pip² females are dorsalised, showing symmetric folds during gastrulation and forming a tube of dorsal cuticle. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4²⁵ show strong ventralisation, producing cuticles with rings of ventral denticle material. The orientation of gastrulation in these embryos is variable and they show muscle movement. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4²⁴¹ show gastrulation movements that are inverted with respect to the intrinsic orientation of the eggshell. The most ventral structures differentiated by most of these embryos are Filzkorper, although some embryos develop ventral denticle material. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4^DE1 form a ventral furrow invagination only at the posterior end. These embryos form rings of denticles at the posterior end.

(Sen et al., 1998)

Mutant embryos respond to injected purified polarizing activity (spz), and produce polarizing activity themselves.

(Schneider et al., 1994)

Injection of snk^Δne.T:ea into the central region of homozygous embryos causes a lateralised/ventralised phenotype.

(Smith and DeLotto, 1994)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Embryos transplanted with perivitelline fluid from Tl² embryos exhibit a polar gastrulation. At the end of embryogenesis an almost complete restoration of pattern along the dorsoventral axis is seen. The point of injection determines the ventralmost point of the restored pattern.

(Stein et al., 1991)

Embryos derived from homozygous females are dorsalised.

(Carroll et al., 1987)

maternal-effect lethal homozygous females are sterile. As in the mutant dl, lethal embryos produced by pip females lack ventral and lateral elements; all cells differentiate like the dorsal-most cells of normal embryos (Anderson and Nusslein-Volhard, 1984). Dorsalization is also observed in the pattern of ftz stripes in pip embryos (Carroll et al., 1987). Embryos are not rescued by injection of wild-type cytoplasm; injection of cytoplasm from ventralized embryos of Tl⁵/+ females, however, partially restores the pip⁺ embryonic pattern, resulting in differentiation of filzkorper in 25-40% of the treated pip embryos (Anderson et al., 1985).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

dec^VA28, pip² has abnormal planar polarity phenotype

(Stein et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Vml^EY21650/Vml^EY21650

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Vml^EY21650/Vml[+]

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Df(2L)Exel7024/+

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by Df(2L)Exel7024/Vml^EY21650/Vml[+]/+

(Zhang et al., 2009)

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, enhanceable | maternal effect by gd^VM90/gd[+]

(Zhang et al., 2009)

NOT Enhanced by

Statement

Reference

pip⁷/pip² has embryonic/first instar larval cuticle | maternal effect phenotype, non-enhanceable | maternal effect by dec-1[+]/dec^VA28

(Zhang et al., 2009)

Other

Statement

Reference

dec^VA28, pip² has embryo phenotype

(Stein et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Vml^EY21650/Vml^EY21650, such that 89.5% of the embryos derived from the double mutant females are completely dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Vml^EY21650/+; 48.0% of embryos derived from the double mutant females are completely dorsalised, and only 1.2% are weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Df(2L)Exel7024/+; 52.1% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying Df(2L)Exel7024/+ and Vml^EY21650/+; 69.5% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is not increased if the females are also carrying dec-1^VA28/+.

The severity of the dorsalisation phenotype seen in embryos derived from pip²/pip⁷ females is increased if the females are also carrying gd^VM90/+; 78.8% of embryos derived from the double mutant females are completely dorsalised, and none are only weakly dorsalised.

(Zhang et al., 2009)

pip², dec-1^VA28 double mutant clones exhibit perturbations in the dorso-ventral axis of most of the embryos derived from egg chambers carrying a ventral clone of mutant follicle cells. Dorsalized phenotypes include a narrowing of the ventral denticle bands and the display of a 'tail-up' phenotype.

(Stein et al., 2008)

pip¹/pip² mutants carrying germ-line clones of ik2¹ mothers produce dorsalised embryos, in the same manner as pip¹/pip² mutants.

(Shapiro and Anderson, 2006)

Embryos derived from Spn27A^ex32/Df(2L)BSC7 ; pip¹/pip² females are completely dorsalised.

(Ligoxygakis et al., 2003)

Perivitelline fluid from embryos laid by spz⁴/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by spz^D1-RPQ/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by Tl^rv13 spz⁴/Tl^rv4 spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D3 spz⁴/ea¹ spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D1 spz²/ea⁴ spz⁴ females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by Tl^rv13/Tl^rv19 females shows polarising activity when injected into the perivitelline space of embryos derived from pip¹/pip² females.