FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spirRD.UASp.GFP
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General Information
Symbol
Dmel\spirRD.UASp.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0191413
Feature type
allele
Associated gene
Dmel\spir
Associated Insertion(s)
Carried in Construct
P{UASp-spir.GFP.RD}
Key Links
Transgenic product class
wild_type
(Rosales-Nieves et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rosales-Nieves et al., 2006)
Mutagen
in vitro construct
(Rosales-Nieves et al., 2006)
Progenitor genotype
Carried in construct
P{UASp-spir.GFP.RD}
Cytology
Description

UASp regulatory sequences drive expression of the spir-RD transcript. The open reading frame is tagged at with GFP.

(Rosales-Nieves et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
spir
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Scer\GAL4nos.UTR.T:Hsim\VP16>spirRD.Scer\UAS.P\T.T:Avic\GFP in spir1/Df(2L)Exel6046 females results in oocytes with an actin mesh and fast streaming is prevented. When fertility is measured, only 12% of the eggs laid by the mutant flies hatch.

      (Quinlan, 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of spirRD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the premature cytoplasmic streaming and abnormal microtubule distribution seen in stage 9 capu2/Df(2L)ed1 oocytes.

      The loss of the actin mesh in the cytoplasm of stage 9 capu2/Df(2L)ed1 oocytes is not rescued by expression of spirRD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.

      (Dahlgaard et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4VP16.nanos.UTR, spirRD.UASp.GFP, Df(2L)Exel6046 partially rescues spir1

      (Quinlan, 2013)

      Scer\GAL4VP16.nanos.UTR/spirRD.UASp.GFP partially rescues spir1/Df(2L)Exel6046

      (Dahlgaard et al., 2007)
      Comments

      Expression of Scer\GAL4nos.UTR.T:Hsim\VP16>spirRD.Scer\UAS.P\T.T:Avic\GFP in spir1/Df(2L)Exel6046 females results in oocytes with an actin mesh and fast streaming is prevented. When fertility is measured, only 12% of the eggs laid by the mutant flies hatch.

      (Quinlan, 2013)

      Expression of spirRD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the premature cytoplasmic streaming seen in spir1/Df(2L)Exel6046 oocytes. The fast cytoplasmic streaming that is normally seen during stages 11-13 is prevented in these oocytes.

      The loss of the actin mesh in the cytoplasm of stage 9 spir1/spir2F oocytes is rescued by expression of spirRD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16. Ectopic actin mesh-like structures are also induced in the nurse cells in these animals.

      (Dahlgaard et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      spirRD.Scer\UAS.P\T.T:Avic\GFP
      spirRD.UASp.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (5)