y promoter sequences at the endogenous locus have been replaced with heterologous eve promoter sequences by targeted gene replacement at yh12w+. In addition, a loxP cassette containing a 593bp fragment that contains the entire gypsy\su(Hw)BR insulator has been inserted 700bp upstream of the transcription start site, in between the endogenous y wing and body enhancers and the heterologous promoter.
adult cuticle (with y1#8)
adult cuticle (with y1)
adult cuticle (with y3c3)
adult cuticle (with y59b)
Body colour: pigmentation score (where 1 = null/nearly null, 5 = wild type/nearly wild type): hemizygote: 3 (wing), 2+-3 (body), yP1\loxP.eve-700gin/y1 : 2+-3 (wing), 2+ (body), yP1\loxP.eve-700gin/y1#8 : 4-4+ (wing), 4-4+ (body), yP1\loxP.eve-700gin/y59b : 4-4+ (wing), 4-4+ (body), yP1\loxP.eve-700gin/y3c3 : 4-4+ (wing), 4-4+ (body).
yeve-700gin has abnormal body color phenotype, suppressible by su(Hw)7/su(Hw)V