Expression of dysfScer\UAS.cJa under the control of Scer\GAL4ptc.PU (limited to the third instar larval stage using Scer\GAL80ts.αTub84B) induces the formation of cuticle folds along the proximo-distal axis of the leg that resembles ectopic joint formation. These joint-like structures are more evident in the tarsal region, although they are also seen in more proximal regions such as the tibia or femur. In the corresponding leg discs a fold is seen along the proximo-distal axis. 24 hours of expression results in increased apoptosis in the larval and prepupal leg discs.
Expression of dysScer\UAS.cJa, under the control of Scer\GAL4btl.PS in stage 16 embryos results in a striking reduction in dorsal branches, dorsal trunk, ganglionic branch, and visceral branch branching. While both dorsal trunk and lateral trunk branches are sometimes fused, the connections are abnormal. The trachea exhibits a severe reduction in secondary and tertiary branching, as well as breaks in lumen formation at the normal site of fusion. Both dorsal branches and ganglionic branches are severely stunted. Misexpression also results in the formation of ectopic tracheal fusion events, connecting tracheal branches that normally never join.