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General Information
Symbol
Dmel\dysfUAS.cJa
Species
D. melanogaster
Name
FlyBase ID
FBal0193498
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dys
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of dysf coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of dysfScer\UAS.cJa under the control of Scer\GAL4ptc.PU (limited to the third instar larval stage using Scer\GAL80ts.αTub84B) induces the formation of cuticle folds along the proximo-distal axis of the leg that resembles ectopic joint formation. These joint-like structures are more evident in the tarsal region, although they are also seen in more proximal regions such as the tibia or femur. In the corresponding leg discs a fold is seen along the proximo-distal axis. 24 hours of expression results in increased apoptosis in the larval and prepupal leg discs.

Expression of dysScer\UAS.cJa, under the control of Scer\GAL4btl.PS in stage 16 embryos results in a striking reduction in dorsal branches, dorsal trunk, ganglionic branch, and visceral branch branching. While both dorsal trunk and lateral trunk branches are sometimes fused, the connections are abnormal. The trachea exhibits a severe reduction in secondary and tertiary branching, as well as breaks in lumen formation at the normal site of fusion. Both dorsal branches and ganglionic branches are severely stunted. Misexpression also results in the formation of ectopic tracheal fusion events, connecting tracheal branches that normally never join.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of dysfScer\UAS.cJa under the control of Scer\GAL4ptc.PU in a Nl1N-ts1 mutant background (shifted to the permissive temperature at the third instar larval stage) results in a uniform and continuous tarsal cuticle with no joints and a fold running along the proximo-distal axis. In the corresponding leg discs a fold is seen along the proximo-distal axis.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dysScer\UAS.cJa
dysfScer\UAS.cJa
dysfUAS.cJa
Name Synonyms
Secondary FlyBase IDs
    References (4)