FlyBase Allele Report: Dmel\dysf[UAS.cJa]

General Information

Symbol

Dmel\dysf^UAS.cJa

Species

D. melanogaster

Name

FlyBase ID

FBal0193498

Feature type

allele

Associated gene

Dmel\dysf

Associated Insertion(s)

Carried in Construct

P{UAS-dysf.J}

Also Known As

UAS-dys

Key Links

Transgenic product class

wild_type

(Jiang and Crews, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jiang and Crews, 2006)

Mutagen

in vitro construct

(Jiang and Crews, 2006)

Progenitor genotype

Carried in construct

P{UAS-dysf.J}

Cytology

Description

UASt regulatory sequences drive expression of dysf coding sequences.

(Jiang and Crews, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dysf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | oogenesis, with Scer\GAL4^slbo.2.6

(Wu et al., 2022)

decreased cell number | oogenesis, with Scer\GAL4^c306

(Wu et al., 2022)

decreased cell number | oogenesis, with Scer\GAL4^slbo.2.6

(Wu et al., 2022)

increased cell death, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

Phenotype Manifest In

adult visceral tracheal branch | embryonic stage 16, with Scer\GAL4^btl.PS

(Jiang and Crews, 2006)

border follicle cell | decreased number, with Scer\GAL4^c306

(Wu et al., 2022)

border follicle cell | decreased number, with Scer\GAL4^slbo.2.6

(Wu et al., 2022)

dorsal tracheal branch primordium | embryonic stage 16, with Scer\GAL4^btl.PS

(Jiang and Crews, 2006)

femur, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

ganglionic tracheal branch primordium | embryonic stage 16, with Scer\GAL4^btl.PS

(Jiang and Crews, 2006)

leg disc | larval stage, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

leg disc | prepupal stage, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

leg joint | ectopic, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

tarsal segment, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

tibia, with Scer\GAL4^ptc.PU

(Córdoba and Estella, 2014)

tracheal dorsal trunk primordium | embryonic stage 16, with Scer\GAL4^btl.PS

(Jiang and Crews, 2006)

tracheal lateral trunk primordium | embryonic stage 16, with Scer\GAL4^btl.PS

(Jiang and Crews, 2006)

Detailed Description

Statement

Reference

Expression of dysf^Scer\UAS.cJa under the control of Scer\GAL4^ptc.PU (limited to the third instar larval stage using Scer\GAL80^ts.αTub84B) induces the formation of cuticle folds along the proximo-distal axis of the leg that resembles ectopic joint formation. These joint-like structures are more evident in the tarsal region, although they are also seen in more proximal regions such as the tibia or femur. In the corresponding leg discs a fold is seen along the proximo-distal axis. 24 hours of expression results in increased apoptosis in the larval and prepupal leg discs.

(Córdoba and Estella, 2014)

Expression of dys^Scer\UAS.cJa, under the control of Scer\GAL4^btl.PS in stage 16 embryos results in a striking reduction in dorsal branches, dorsal trunk, ganglionic branch, and visceral branch branching. While both dorsal trunk and lateral trunk branches are sometimes fused, the connections are abnormal. The trachea exhibits a severe reduction in secondary and tertiary branching, as well as breaks in lumen formation at the normal site of fusion. Both dorsal branches and ganglionic branches are severely stunted. Misexpression also results in the formation of ectopic tracheal fusion events, connecting tracheal branches that normally never join.

(Jiang and Crews, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^c306, dysf^UAS.cJa has decreased cell number | oogenesis phenotype, suppressible by Scer\GAL4^c306/hop^UAS.cHa

(Wu et al., 2022)

Scer\GAL4^c306, dysf^UAS.cJa has decreased cell number | oogenesis phenotype, suppressible by Scer\GAL4^c306/upd1^UAS.cZa

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has abnormal cell migration | oogenesis phenotype, suppressible | partially by Pen^{UASp.NRE-WT.Tag:MYC}, Scer\GAL4^slbo.2.6

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has abnormal cell migration | oogenesis phenotype, suppressible | partially by Nup153^NP2104

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has abnormal cell migration | oogenesis phenotype, suppressible | partially by Nup153^GD10636, Scer\GAL4^slbo.2.6

(Wu et al., 2022)

Other

Statement

Reference

Scer\GAL4^c306, dysf^UAS.cJa, hop^UAS.cHa has abnormal cell migration | oogenesis phenotype

(Wu et al., 2022)

Scer\GAL4^c306, dysf^UAS.cJa, upd1^UAS.cZa has abnormal cell migration | oogenesis phenotype

(Wu et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^c306, dysf^UAS.cJa has border follicle cell | decreased number phenotype, suppressible by Scer\GAL4^c306/hop^UAS.cHa

(Wu et al., 2022)

Scer\GAL4^c306, dysf^UAS.cJa has border follicle cell | decreased number phenotype, suppressible by Scer\GAL4^c306/upd1^UAS.cZa

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has border follicle cell | decreased number phenotype, suppressible | partially by Pen^{UASp.NRE-WT.Tag:MYC}, Scer\GAL4^slbo.2.6

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has border follicle cell | decreased number phenotype, suppressible | partially by Nup153^NP2104

(Wu et al., 2022)

Scer\GAL4^slbo.2.6, dysf^UAS.cJa has border follicle cell | decreased number phenotype, suppressible | partially by Nup153^GD10636, Scer\GAL4^slbo.2.6

(Wu et al., 2022)

Other

Statement

Reference

Scer\GAL4^c306, dysf^UAS.cJa, hop^UAS.cHa has border follicle cell phenotype

(Wu et al., 2022)

Scer\GAL4^c306, dysf^UAS.cJa, upd1^UAS.cZa has border follicle cell phenotype

(Wu et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dysf^Scer\UAS.cJa under the control of Scer\GAL4^ptc.PU in a N^l1N-ts1 mutant background (shifted to the permissive temperature at the third instar larval stage) results in a uniform and continuous tarsal cuticle with no joints and a fold running along the proximo-distal axis. In the corresponding leg discs a fold is seen along the proximo-distal axis.

(Córdoba and Estella, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9592

w^*; P{UAS-dysf.J}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dys^Scer\UAS.cJa

dysf^Scer\UAS.cJa

dysf^UAS.cJa

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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