EcollacZegh.PS expression is observed in the embryonic, larval, and adult central nervous system. In the larval ventral nerve cord, EcollacZegh.PS is expressed in one neuron per abdominal hemisegment and two per thoracic hemisegment; these neurons project to the central brain. These neurons are also positive for expression of the ap GAL4 driver ScerGAL4ap-md544.