FlyBase Allele Report: Dmel\Delta::Ser[Delta-E.Ser-I.UAS]

General Information

Symbol

Dmel\Delta::Ser^{Delta-E.Ser-I.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0194256

Feature type

allele

Associated gene

Delta::Ser

Associated Insertion(s)

Carried in Construct

P{UAS-Delta::Ser.L}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Li and Baker, 2004)

Progenitor genotype

Carried in construct

P{UAS-Delta::Ser.L}

Cytology

Description

UASt regulatory sequences drive expression of a Dl-Ser fusion protein which comprises the extracellular and transmembrane domains of Dl and the intracellular domain of Ser. Nucleotides encoding Met619 and Lys620 (at the cytoplasmic face of the transmembrane domain) of the Dl protein are replaced with an XbaI restriction site (which encodes Ser and Arg). The initiating ATG codon of Dl is replaced with a BspH1 restriction site which results in replacing His2 with Asn. The endogenous Dl 5'UTR is replaced with a HindIII-NcoI fragment of the Xenopus β-globin gene leader. The 3'UTR of the Ser cDNA is truncated to 17bp 3' of the stop codon and SV40 polyadenylation sequences are present.

(Li and Baker, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Delta::Ser

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^h-H10

(Li and Baker, 2004)

Phenotype Manifest In

eye, with Scer\GAL4^sca-109-68

(Li and Baker, 2004)

macrochaeta & thorax | supernumerary, with Scer\GAL4^sca-537.4

(Li and Baker, 2004)

macrochaeta & thorax | supernumerary | somatic clone | cell autonomous, with Scer\GAL4^Ubx.PdC

(Li and Baker, 2004)

ommatidium & eye disc, with Scer\GAL4^h-H10

(Li and Baker, 2004)

photoreceptor cell R8 & eye disc, with Scer\GAL4^h-H10

(Li and Baker, 2004)

Detailed Description

Statement

Reference

Expression of Dl::Ser^{Dl-E.Ser-I.Scer\UAS} (line A10, P{UAS-Dl::Ser.L}A10) under the control of Scer\GAL4^sca-537.4 results in a mild neurogenic phenotype: a few extra macrochaetae form on the thorax.

Expression of Dl::Ser^{Dl-E.Ser-I.Scer\UAS} (line A10, P{UAS-Dl::Ser.L}A10) under the control of Scer\GAL4^h-H10 is weakly neurogenic in the eye imaginal disc - multiple R8 cells are seen in 4% of ommatidia - and a disorganised pattern of ommatidia associated with the acceleration of the morphogenetic furrow.

Clonal expression of Dl::Ser^{Dl-E.Ser-I.Scer\UAS} (line A10, P{UAS-Dl::Ser.L}A10) in thoracic cells (using Scer\GAL4^Ubx.PdC) leads to extra thoracic bristle differentiation in a cell autonomous manner.

Expression of Dl::Ser^{Dl-E.Ser-I.Scer\UAS} (line A10, P{UAS-Dl::Ser.L}A10) in differentiating R8 cells (using Scer\GAL4^sca-109-68) leads to a rough eye of similar size to wild type.

(Li and Baker, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^sca-109-68/Delta::Ser^{Delta-E.Ser-I.UAS} is a non-suppressor of eye | male phenotype of N^spl-1

(Li and Baker, 2004)

Scer\GAL4^sca-109-68/Delta::Ser^{Delta-E.Ser-I.UAS} is a non-suppressor of photoreceptor cell R8 & eye disc | male phenotype of N^spl-1

(Li and Baker, 2004)

Scer\GAL4^sca-109-68/Delta::Ser^{Delta-E.Ser-I.UAS} is a non-suppressor of ommatidium & eye disc | male phenotype of N^spl-1

(Li and Baker, 2004)

Scer\GAL4^sca-109-68/Delta::Ser^{Delta-E.Ser-I.UAS} is a non-suppressor of photoreceptor cell & eye disc | male phenotype of N^spl-1

(Li and Baker, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dl::Ser^{Dl-E.Ser-I.Scer\UAS} (line A10, P{UAS-Dl::Ser.L}A10) in R8 cells (using Scer\GAL4^sca-109-68) does not rescue the adult eye or eye imaginal disc defects of hemizygous N^spl-1 males.

(Li and Baker, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Delta::Ser^{Delta-E.Ser-I.UAS}

Delta::Ser^{Dl-E.Ser-I.UAS}

Dl::Ser^{Dl-E.Ser-I.Scer\UAS}

Dl::Ser^{Dl-E.Ser-I.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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