FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vha100-12
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General Information
Symbol
Dmel\Vha100-12
Species
D. melanogaster
Name
FlyBase ID
FBal0194361
Feature type
allele
Associated gene
Dmel\Vha100-1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Hiesinger et al., 2005)
Progenitor genotype
Cytology
Description

Amino acid replacement: A?V.

(Hiesinger et al., 2005)

Point mutation somewhere in exon 7.

(Hiesinger et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:29,135,839..29,135,839 [+]
Nucleotide change:

C29135839T

Amino acid change:

A407V | Vha100-1-PA; A410V | Vha100-1-PB; A429V | Vha100-1-PC; A410V | Vha100-1-PD; A429V | Vha100-1-PE; A407V | Vha100-1-PF; A424V | Vha100-1-PG; A424V | Vha100-1-PI; A424V | Vha100-1-PJ; A410V | Vha100-1-PK

Reported amino acid change:

A?V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Site of amino acid change inferred from surrounding amino acid sequence.

(Hiesinger et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vha100-12 homozygous whole-eye clones in the adult lose on-off retinal transients in response to short light pulses and have a significant reduction in light sensitivity compared to controls.

      (Zhao et al., 2018)

      Vha100-12 mutant photoreceptor terminals show increased accumulation of autophagic vacuoles compared to controls.

      (Tian et al., 2015)

      Vha100-12 in combination with Vha100-11, Vha100-13, Vha100-14, Vha100-15 or Df(3R)3450) develop to late embryonic stages, but fail to hatch. The same phenotype is seen when these embryos are born to born to mothers with a Vha100-11 homozygous germ-line.

      Flies in which the retina is homozygous for Vha100-12 (made using the 'ey-flp' system described in FBrf0125463) consistently fail to walk toward light in a phototaxis assay. Electro-retinograms from these animals display normal depolarization in response to light stimuli, but exhibit a complete loss of 'on' and 'off' transients, indicative of a failure to evoke a postsynaptic response.

      The frequency and amplitude of mini excitatory postsynaptic currents at the neuromuscular junctions of Vha100-12/Vha100-13 embryos is normal. However, excitatory postsynaptic currents induced by stimulation of the motor nerve supplying muscle 6 in these embryos have significantly lower amplitudes than in wild-type. As in wild-type, postsynaptic currents are induced in response to application of a hypertonic solution at the neuromuscular junction.

      Assays of dye-uptake (FM1-43) into boutons at neuromuscular junctions indicate a significant reduction in vesicle cycling at unstimulated junctions of Vha100-12/Vha100-13 embryos compared to wild-type.

      (Hiesinger et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PF/Vha100-12 is a non-suppressor of eye phenotype of Syx1AUAS.cBa

      (Hiesinger et al., 2005)

      Scer\GAL4GMR.PF/Vha100-12 is a non-suppressor of ommatidium phenotype of Syx1AUAS.cBa

      (Hiesinger et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The rough eye phenotype of Syx1AScer\UAS.cBa; Scer\GAL4GMR.PF flies raised at 18oC is not suppressed when the eye is made homozygous for Vha100-12.

      (Hiesinger et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vha100-12
      (Zhao et al., 2018, Tian et al., 2015)
      v1002
      (Hiesinger et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)