FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vha100-13
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General Information
Symbol
Dmel\Vha100-13
Species
D. melanogaster
Name
FlyBase ID
FBal0194362
Feature type
allele
Associated gene
Dmel\Vha100-1
Associated Insertion(s)
Carried in Construct
Also Known As
v1003
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Hiesinger et al., 2005)
Progenitor genotype
Cytology
Description

Amino acid replacement: G?R.

(Hiesinger et al., 2005)

Point mutation somewhere in exon 7, 3' to the point mutation in Vha100-12.

(Hiesinger et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:29,135,859..29,135,859 [+]
Nucleotide change:

G29135859C

Amino acid change:

G414R | Vha100-1-PA; G417R | Vha100-1-PB; G436R | Vha100-1-PC; G417R | Vha100-1-PD; G436R | Vha100-1-PE; G414R | Vha100-1-PF; G431R | Vha100-1-PG; G431R | Vha100-1-PI; G431R | Vha100-1-PJ; G417R | Vha100-1-PK

Reported amino acid change:

G?R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Site of amino acid change inferred from surrounding amino acid sequence.

(Hiesinger et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vha100-13 heterozygotes are viable.

      (Şentürk et al., 2019)

      Flies in which the retina is homozygous for Vha100-13 (made using the 'ey-flp' system described in FBrf0125463) consistently fail to walk toward light in a phototaxis assay. Electro-retinograms from these animals display normal depolarization in response to light stimuli, but exhibit a complete loss of 'on' and 'off' transients, indicative of a failure to evoke a postsynaptic response.

      The frequency and amplitude of mini excitatory postsynaptic currents at the neuromuscular junctions of Vha100-12/Vha100-13 embryos is normal. However, excitatory postsynaptic currents induced by stimulation of the motor nerve supplying muscle 6 in these embryos have significantly lower amplitudes than in wild-type. As in wild-type, postsynaptic currents are induced in response to application of a hypertonic solution at the neuromuscular junction.

      (Hiesinger et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Vha100-13/Vha100-1[+] is a suppressor | partially of increased mortality during development phenotype of Ubqn1

      (Şentürk et al., 2019)
      Other
      Statement
      Reference

      Ubqn1, Vha100-13/Vha100-1[+] has lethal - all die before end of P-stage phenotype

      (Şentürk et al., 2019)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Vha100-13/Vha100-1[+] is a suppressor | partially of embryonic/larval fat body | somatic clone | third instar larval stage phenotype of Ubqn1

      (Şentürk et al., 2019)

      Vha100-13/Vha100-1[+] is a suppressor | partially of lysosome | somatic clone | third instar larval stage phenotype of Ubqn1

      (Şentürk et al., 2019)
      NOT Suppressor of
      Statement
      Reference

      Vha100-13/Vha100-1[+] is a non-suppressor of embryonic/larval salivary gland | somatic clone | third instar larval stage phenotype of Ubqn1

      (Şentürk et al., 2019)

      Vha100-13/Vha100-1[+] is a non-suppressor of endoplasmic reticulum | somatic clone | third instar larval stage phenotype of Ubqn1

      (Şentürk et al., 2019)

      Vha100-13/Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Syx1AUAS.cBa

      (Hiesinger et al., 2005)

      Vha100-13/Scer\GAL4GMR.PF is a non-suppressor of ommatidium phenotype of Syx1AUAS.cBa

      (Hiesinger et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Ubqn1 clones in the third instar larval salivary gland show aberrant morphology and accumulation of endoplasmic reticulum (sqh-EYFP-ER marker), which are not suppressed by Vha100-13 heterozygosity.

      Ubqn1 clones in the third instar larval fat body show a severely decreased Lysotracker staining (marker of acidic lysosomes), which is significantly suppressed by Vha100-13 heterozygosity.

      (Şentürk et al., 2019)

      The rough eye phenotype of Syx1AScer\UAS.cBa; Scer\GAL4GMR.PF flies raised at 18oC is not suppressed when the eye is made homozygous for Vha100-13.

      (Hiesinger et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vha100-13
      v1003
      (Şentürk et al., 2019, Hiesinger et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)