FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vps25K2
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General Information
Symbol
Dmel\Vps25K2
Species
D. melanogaster
Name
FlyBase ID
FBal0194621
Feature type
allele
Associated gene
Dmel\Vps25
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Herz et al., 2006)
Mutagen
ethyl methanesulfonate
(Herz et al., 2006)
Progenitor genotype
Cytology
Description

A transversion from T to A in the second base of the only Vps25 intron causes a splicing defect and, subsequently, premature termination of translation by an in-frame stop codon in the intron.

(Herz et al., 2006)

Nucleotide substitution: T29A.

(Herz et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:8,625,577..8,625,577 [+]
Nucleotide change:

T8625577A

Reported nucleotide change:

T29A

Comment:

T to A mutation in the second base of the intron.

(Herz et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  carcinoma
      CEA
      (Herz et al., 2006)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vps25N55/Vps25K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type.

      TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25K2 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25K2 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages.

      (Herz et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Vps25K2 is a suppressor of increased cell death phenotype of hidGMR.PG

      (Herz et al., 2006)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Vps25K2 is a suppressor of eye phenotype of hidGMR.PG

      (Herz et al., 2006)

      Vps25N55/Vps25K2 is a suppressor of eye | somatic clone | cell non-autonomous phenotype of hidGMR.PG

      (Herz et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Vps25K2 moderate-strongly suppresses the WGMR.PG eye phenotype.

      In Vps25N55/Vps25K2 mosaic eyes, in a WGMR.PG background, the rescued tissue is genetically wild-type or heterozygous, suggesting that Vps25N55 and Vps25K2 mutant tissue does not contribute to the suppression of WGMR.PG.

      (Herz et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Vps25K2 is rescued by Vps25UAS.cHa

      (Herz et al., 2006)

      Vps25K2 is rescued by Vps25hs.PH

      (Herz et al., 2006)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      K2
      (Herz et al., 2006)
      Vps25K2
      su(GMR-hid)K2
      (Herz et al., 2006)
      vps25K2
      (Herz et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)