FlyBase Allele Report: Dmel\LIMK1[kd.UAS.Tag:MYC]

General Information

Symbol

Dmel\LIMK1^{kd.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0194766

Feature type

allele

Associated gene

Dmel\LIMK1

Associated Insertion(s)

Carried in Construct

P{UAS-LIMK1.kd}

Key Links

Transgenic product class

inframe_deletion

(Ang et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ang et al., 2006)

Mutagen

in vitro construct

(Ang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-LIMK1.kd}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of LIMK1, which is lacking the amino-terminal regulatory domain (amino acid residues 1--305), which has 2 copies of the Tag:MYC tag at the carboxy terminus.

(Ang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

LIMK1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Abe et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^SG18.1

(Ang et al., 2006)

Phenotype Manifest In

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Abe et al., 2014)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Abe et al., 2014)

antennal lobe, with Scer\GAL4^SG18.1

(Ang et al., 2006)

antennal lobe glomerulus | ectopic, with Scer\GAL4^SG18.1

(Ang et al., 2006)

antennal lobe glomerulus DM6, with Scer\GAL4^SG18.1

(Ang et al., 2006)

antennal lobe glomerulus VA6, with Scer\GAL4^SG18.1

(Ang et al., 2006)

Detailed Description

Statement

Reference

The mushroom bodies of flies expressing LIMK1^{kd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107 show axonal growth defects at the lobe region but not the peduncle. Scer\GAL4^ey-OK107>Pak^{Scer\UAS.T:Myr-Src64B} expressing clones display strong ectopic F-actin at the α/β branching point, as well as toward the distal region of the medial axons.

(Abe et al., 2014)

Animals overexpressing LIMK1^{kd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^SG18.1 have antennal lobes that are elongated dorsally compared to wild-type animals. The structures of many glomeruli within the LIMK1^{kd.Scer\UAS.T:Hsap\MYC}-overexpressing lobes are disrupted; although the lateral glomeruli (VA1d and DA4) maintain a wild-type position, medially-located glomeruli (VA6 and DM6) can only sometimes be identified.

Scer\GAL4^SG18.1>LIMK1^{kd.Scer\UAS.T:Hsap\MYC} animals show globose structures at the midline of the antennal commissure, a region that only contains axons in wild-type animals. These structures are ectopic glomeruli as evidenced by innervation of the midline by dorsomedial dendrites and extension of dorsomedial arbors to the midline.

(Ang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has abnormal neuroanatomy phenotype, suppressible by tsr^S3A.cA.UAS, Scer\GAL4^SG18.1

(Ang et al., 2006)

NOT suppressed by

Statement

Reference

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has abnormal neuroanatomy phenotype, non-suppressible by tsr^UAS.cAa, Scer\GAL4^SG18.1

(Ang et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe phenotype, suppressible by tsr^S3A.cA.UAS, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus DM6 phenotype, suppressible by tsr^S3A.cA.UAS, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus VA6 phenotype, suppressible by tsr^S3A.cA.UAS, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus | ectopic phenotype, suppressible by tsr^S3A.cA.UAS, Scer\GAL4^SG18.1

(Ang et al., 2006)

NOT suppressed by

Statement

Reference

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe phenotype, non-suppressible by tsr^UAS.cAa, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus DM6 phenotype, non-suppressible by tsr^UAS.cAa, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus VA6 phenotype, non-suppressible by tsr^UAS.cAa, Scer\GAL4^SG18.1

(Ang et al., 2006)

LIMK1^{kd.UAS.Tag:MYC}, Scer\GAL4^SG18.1 has antennal lobe glomerulus | ectopic phenotype, non-suppressible by tsr^UAS.cAa, Scer\GAL4^SG18.1

(Ang et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of tsr^{S3A.cA.Scer\UAS} and LIMK1^{kd.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^SG18.1, suppresses the disruption of glomerular structures and presence of ectopic glomeruli at the midline, seen when LIMK1^{kd.Scer\UAS.T:Hsap\MYC} is expressed alone, in 83% of antennal lobes. However, coexpression of tsr^Scer\UAS.cAa does not suppress the Scer\GAL4^SG18.1>LIMK1^{kd.Scer\UAS.T:Hsap\MYC} phenotype.

(Ang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

LIMK1^{kd.Scer\UAS.T:Hsap\MYC}

LIMK1^{kd.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

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